The microbiome has emerged in the last decade as critical aspect of human biology, biofilms, the environment more. Microbes out number human cells 10 to 1 in our bodies and play a variety of roles in metabolism, immune function and many yet to be discovered aspects of human physiology. As most are below the resolving power of light microscope their analysis requires dyes to illuminate their location and status. Marker Gene offers a number of kits and reagents to label and differentiate microbes, including kits to assess their viability, vitality, redox potential and number.
This high copy number eukaryotic vector, pDC57, expresses a mutant luciferase gene under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. This vector encodes a mutant enzyme that catalyzes the production of long wavelength (red) light (Em:605) from D-luciferin, M0237. Learn More
Employs the highly sensitive substrate FDG, M0250,
a di-β-galactoside conjugate of the dye fluorescein, found to more effectively detect E. Coli and other coliforms through both basal and induced β-galactosidase activity.
Unlike other commercially available luciferase kits using green light emitting (562 nm) firefly analogs, this luminescent assay kit produces a red light (EM 619 nm) for measurement of cell viability based upon the generation of ATP in metabolically active cells. Learn More