MitoPT™ TMRE Assay Kit – 500 tests

Product ID: M1698



Unit SizePriceQuantity 
1kit
$264.55

Availability: In stock


Description

Detects mitochondrial membrane depolarization using the potentiometric fluorescent dye tetramethylrhodamine ethyl ester(TMRE) which emits a red fluorescence (em. 574 nm) in negatively charged, polarized mitochondria.

The MitoPT™ TMRE assay can be used in conjunction with existing apoptosis or metabolic stress protocols. Each kit includes the MitoPT™- TMRE potentiometric reagent, a wash buffer, and the mitochondrial membrane depolarizer Carbonylcyanide m- chlorophenylhydrazone (CCCP). TMRE-stained cells can be analyzed using flow cytometry, fluorescence microscopy, and fluorescence plate reader evaluation methodology.

Technical Data
SKU M1698
Unit Size 1kit
Detection Method Fluorescence

References and Citations

References:

  • O'Reilly CM, Fogarty KE, Drummond RM, Tuft RA, Walsh JV. (2003) "Quantitative analysis of spontaneous mitochondrial depolarizations." Biophys. J. 85(5): 3350–3357.
  • Collins TJ, Berridge MJ, Lipp P, Bootman MD. (2002) "Mitochondria are morphologically and functionally heterogeneous within cells." EMBO J. 21(7): 1616-1627.
  • Duchen MR, Leyssens A, Crompton M. (1998) "Transient mitochondrial depolarizations reflect focal sarcoplasmic reticular calcium release in single rat cardiomyocytes." J. Cell Biol. 142(4): 975-988.
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