MitoPT™ TMRM Assay Kit – 500 tests

Product ID: M1699



Unit SizePriceQuantity 
1kit
$264.55

Availability: In stock


Description

Detects mitochondrial membrane depolarization using the fluorescent dye TMRM which accumulates within negatively charged mitochondrial organelles, exhibiting a red-orange fluorescence (em. 573 nm) that increases dramatically when excited at 548 nm.

The MitoPT™ TMRM assay can be used in conjunction with existing apoptosis or metabolic stress protocols. Each kit includes the TMRM potentiometric dye reagent, a 10X wash buffer for removing any excess TMRM dye after staining, and the generic mitochondrial membrane depolarizer, Carbonylcyanide m- chlorophenylhydrazone (CCCP). TMRM-stained cells can be analyzed using flow cytometry, fluorescence microscopy, and fluorescence plate reader evaluation methodology.

Technical Data
SKU M1699
Unit Size 1kit
Detection Method Fluorescence

References and Citations

References:

  • Honarnejad K, Daschner A, Gehring AP, Szybinska A, Giese A, Kuznicki J, Bracher F, Herms J. (2014) "Identification of tetrahydrocarbazoles as novel multifactorial drug candidates for treatment of Alzheimer's disease." Translational Psychiatry 4(12): e489.
  • Plasek J, Denksteinova B, Sureau F. (1993) "Assessment of membrane potential using confocal microspectrofluorometry." Journal of Fluorescence 3(3): 157-159.
  • Loew LM. (1996) "Potentiometric dyes: New modalities for optical imaging of membrane potential." Proceedings of SPIE-The International Society for Optical Engineering 2678(Optical Diagnostics of Living Cells and Biofluids) 80-87.
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