Fluorescein methotrexate

Product ID: M4349

Unit SizePriceQuantity 
1 mg

Availability: In stock


Fluorescein methotrexate is useful for studying antimetabolite resistance and spontaneous gene amplification.

Fluorescent probe for visualization of biochemical networks in living cells. It is used for studying antimetabolite resistance and spontaneous gene amplification. The quantitative binding of fluorescein methotrexate to dihydrofolate reductase (DHFR) enables researchers to isolate cells based on DHFR expression.

Technical Data
SKU M4349
CAS Number 71016-04-1
Unit Size 1 mg
Alternative Names MTX, fluorescein, triammonium salt; Methotrexate, fluorescein, triammonium salt
Absorption 497
Emission Wavelength 516
Extinction 63000
Detection Method Fluorescence
Molecular Formula C46H54N14O9S
Molecular Weight 979.08
Soluble In DMSO, DMF, Methanol and Water
Storage Conditions -20C, protect from light, dessicate

References and Citations


  • Li T, Ito K, Horie T. (2003) "Transport of fluorescein methotrexate by multidrug resistance-associated protein 3 in IEC-6 cells." Am J Physiol Gastrointest Liver Physiol 285, G602-10
  • Terlouw SA, Graeff C, Smeets PH, Fricker G, Russel FG, Masereeuw R, Miller DS. (2002) "Short- and long-term influences of heavy metals on anionic drug efflux from renal proximal tubule." J Pharmacol Exp Ther 301, 578-585
  • Subramaniam R, Desveaux D, Spickler C, Michnick SW, Brisson N. (2001)"Direct visualization of protein interactions in plant cells." Nat Biotechnol 19, 769-772
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