4-Methylumbelliferyl β-D-galactopyranoside (MUG)

Product ID: M0241

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Bright blue fluorogenic β-galactosidase substrate, especially useful for in vitro assays of cloned lacZ expression.

This fluorogenic substrate is especially useful for in vitro assays of β-galactosidase activity arising from cloned lacZ expression in mammalian cells.

Technical Data
SKU M0241
CAS Number 6160-78-7
Unit Size 1g
Absorption 316nm
Emission Wavelength 376
Extinction 14
Detection Method Fluorescence
Molecular Formula C₁₆H₁₈O₈
Molecular Weight 338.3
Soluble In H2O, DMSO
Storage Conditions -20C, Desiccated
Notes Absorbance 360nm/Emission 449nm/E 19K of product 4-methylumbelliferone after enzyme hydrolysis.

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References and Citations


  • Brumm PJ, Hermanson S, Gowda K, Xie D, And Mead DA, (2015) "Clostridium thermocellum Cel5L – Cloning and Characterization of a New, Thermostable GH5 Cellulase" International Journal of Biochemistry Research & Review 6: 62-74.
  • Jackson K, Fan ZH. (2013) "Cell-Free Protein Synthesis in Miniaturized Array Devices and Effects of Device Orientation." J Lab Autom. 19(4):366-374.
  • Dubrovsky JG, Napsucialy-Mendivil S, Duclercq J, Cheng Y, Shishkova S, Ivanchenko MG, Friml J, Murphy AS, Benková E. (2011) "Auxin minimum defines a developmental window for lateral root initiation." New Phytol. 191(4):970-83.
  • Khnouf R, Olivero D, Jin S, Fan ZH.(2010) "Miniaturized fluid array for high-throughput protein expression." Biotechnol Prog. 26(6):1590-6
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  • "A microplate fluorimetric assay for transfection of the beta-galactosidase reporter gene." Rakhmanova VA, MacDonald RC. Anal. Biochem. 257: 234-237 (1998).
  • "Human lysosomal beta-galactosidase-cathepsin A complex: definition of the beta-galactosidase-binding interface on cathepsin A." Pshezhetsky AV, Elsliger MA, Vinogradova MV, Potier M. Biochemistry 34: 2431-2440 (1995).
  • "Highly sensitive fluorimetric enzyme immunoassay for prostaglandin H synthase solubilized from cultured cells." Ruan KH, Kulmacz RJ, Wilson A, Wu KK. J Immunol. Methods 162: 23-30 (1993).
  • "Selective inactivation of eukaryotic beta-galactosidase in assays for inhibitors of HIV-1 TAT using bacterial beta-galactosidase as a reporter enzyme." Young DC, Kingsley SD, Ryan KA, Dutko FJ. Anal. Biochem. 215: 24-30 (1993).
  • "Activation of cAMP-responsive genes by stimuli that produce long-term facilitation in Aplysia sensory neurons." Kaang BK, Kandel ER, Grant SG. Neuron 10: 427-435 (1993).
  • "Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer." Price J, Turner D, Cepko C. Proc. Natl. Acad. Sci. USA 84: 156-160 (1987).
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