4-Methylumbelliferyl β-D-glucuronide (MUGlcU)

Product ID: M0240



Unit SizePriceQuantity 
25mg
$44.86
  • Buy 5 for $35.88 each and save 21%

Availability: In stock


Description

Common blue fluorogenic GUS substrate for measuring cloned activity in plant lysate, homogenate, leaf disk or whole plant assays.

This fluorogenic substrate is especially useful for in vitro assays of β-glucuronidase activity arising from cloned GUS gene expression in plant lysate, homogenate, leaf disk or whole plant assays. β-Glucuronidase (GUS) encoded by the gusA gene from E.coli can cleave this substrate and generate the fluorogenic product 4-MU, that can be visualized or detected by irradiation with UV light. The fluorescence assay allows quantitation of GUS activity by means of a fluorimeter in protein extracts with excitation at 365nm (UV) and a peak emission at 455nm (blue). The fluorescence is optimal at pH values above 8-9.

Technical Data
SKU M0240
CAS Number 6160-80-1
Unit Size 25mg
Absorption 316nm
Emission Wavelength 375
Extinction 12
Detection Method Fluorescence
Molecular Formula C₁₆H₁₆O₉
Molecular Weight 352.3
Soluble In H2O (pH >six); DMSO
Storage Conditions -20C, Desiccated
Notes Absorbance 360nm/Emission 449nm/E 19K of product 4-methylumbelliferone after enzyme hydrolysis.

References and Citations

Citations:

  • Chandrasekaran A, Singh AK. (2014) "One-pot, microscale cell-free enzyme expression and screening." Methods Mol Biol. 1118:55-69.
  • Geary JR, Nijak GM Jr, Larson SL, Talley JW. (2011) "Hydrolysis of the soluble fluorescent molecule carboxyumbelliferyl-beta-D-glucuronide by E. coli beta-glucuronidase as applied in a rugged, in situ optical sensor." Enzyme Microb Technol. 49(1):6-10
  • Fan ZH, Khnouf R, Mei Q, Jin S. (2010) "A fluid array device for high-throughput protein synthesis." 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Groningen, The Netherlands.
  • Khnouf R, Olivero D, Shouguang J, Fan ZH. (2010) “Miniaturized fluid array for high-throughput protein expression.” Biotechnology Progress Vol 26, Issue 6 pp 1590-1596.
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References:

  • "GUS Protocols: Using the GUS Gene as a Reporter of Gene Expression." Naleway, JJ, S.R.Gallagher, Ed., Acad. Press, pp. 61-76 (1992). US Federal Register 57, 24744.
  • Tryland I, Fiksdal L. (1998) "Enzyme Characteristics of β-d-Galactosidase- and β-d-Glucuronidase-Positive Bacteria and Their Interference in Rapid Methods for Detection of Waterborne Coliforms and Escherichia coli." Appl. Environ. Microbiol. 64(3): 1018–1023.
  • Lois C. Shadix, Michele E. Dunnigan, Eugene W. Rice. (1993) "Detection of Escherichia coli by the nutrient agar plus 4-methylumbelliferyl β-D-glucuronide (MUG) membrane filter method." Canadian Journal of Microbiology 39(11): 1066-1070.
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