4-Methylumbelliferyl β-D-glucuronide (MUGlcU)

Product ID: M0240

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Common blue fluorogenic GUS substrate for measuring cloned activity in plant lysate, homogenate, leaf disk or whole plant assays.

This fluorogenic substrate is especially useful for in vitro assays of β-glucuronidase activity arising from cloned GUS gene expression in plant lysate, homogenate, leaf disk or whole plant assays. β-Glucuronidase (GUS) encoded by the gusA gene from E.coli can cleave this substrate and generate the fluorogenic product 4-MU, that can be visualized or detected by irradiation with UV light. The fluorescence assay allows quantitation of GUS activity by means of a fluorimeter in protein extracts with excitation at 365nm (UV) and a peak emission at 455nm (blue). The fluorescence is optimal at pH values above 8-9.

Technical Data
SKU M0240
CAS Number 6160-80-1
Unit Size 25mg
Absorption 316nm
Emission Wavelength 375
Extinction 12
Detection Method Fluorescence
Molecular Formula C₁₆H₁₆O₉
Molecular Weight 352.3
Soluble In H2O (pH >six); DMSO
Storage Conditions -20C, Desiccated
Notes Absorbance 360nm/Emission 449nm/E 19K of product 4-methylumbelliferone after enzyme hydrolysis.

References and Citations


  • Chandrasekaran A, Singh AK. (2014) "One-pot, microscale cell-free enzyme expression and screening." Methods Mol Biol. 1118:55-69.
  • Geary JR, Nijak GM Jr, Larson SL, Talley JW. (2011) "Hydrolysis of the soluble fluorescent molecule carboxyumbelliferyl-beta-D-glucuronide by E. coli beta-glucuronidase as applied in a rugged, in situ optical sensor." Enzyme Microb Technol. 49(1):6-10
  • Fan ZH, Khnouf R, Mei Q, Jin S. (2010) "A fluid array device for high-throughput protein synthesis." 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Groningen, The Netherlands.
  • Khnouf R, Olivero D, Shouguang J, Fan ZH. (2010) “Miniaturized fluid array for high-throughput protein expression.” Biotechnology Progress Vol 26, Issue 6 pp 1590-1596.
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  • "GUS Protocols: Using the GUS Gene as a Reporter of Gene Expression." Naleway, JJ, S.R.Gallagher, Ed., Acad. Press, pp. 61-76 (1992). US Federal Register 57, 24744.
  • Tryland I, Fiksdal L. (1998) "Enzyme Characteristics of β-d-Galactosidase- and β-d-Glucuronidase-Positive Bacteria and Their Interference in Rapid Methods for Detection of Waterborne Coliforms and Escherichia coli." Appl. Environ. Microbiol. 64(3): 1018–1023.
  • Lois C. Shadix, Michele E. Dunnigan, Eugene W. Rice. (1993) "Detection of Escherichia coli by the nutrient agar plus 4-methylumbelliferyl β-D-glucuronide (MUG) membrane filter method." Canadian Journal of Microbiology 39(11): 1066-1070.
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