MarkerGene™ Multiple Drug Resistance Microtiterplate Assay Kit

Product ID: M1580



Unit SizePriceQuantity 
1kit
$258.50
  • Buy 5 for $206.80 each and save 20%

Availability: In stock


Description

This kit contains protocols and reagents for use in the detection of Multiple Drug Resistance within cells.

Multiple drug resistance is characterized by over-expression of membrane transport proteins ABCG2 and ABCB1 that induce efflux of anticancer drugs out of the cell and subsequently cause tumor cell resistance to those drugs. Overcoming MDR has become a major challenge for chemotherapy. Early detection of drug resistant tumor cells may be critical in successful treatment of tumors. In addition, multiple drug resistance has become important for treatment of several other diseases such as tuberculosis, cholera, malaria and HIV-AIDS. The MarkerGeneTM Multiple Drug Resistance Microtiterplate Assay Kit is a high-throughput assay system based upon measurement of efflux of a green fluorescent dye that is known to bind to the MDR transporters ABCG2 and ABCB1 in a manner similar to many chemotherapeutic drugs. The efflux of this dye can be easily measured by its fluorescence in the media (EM 504nm, EX 538nm).

Technical Data
SKU M1580
Unit Size 1kit
Detection Method Fluorescence

References and Citations

Citations:

  • Sasahira T, Nishiguchi Y, Fujiwara R, Kurihara M, Kirita T, Bosserhoff AK, Kuniyasu H. (2016) "Storkhead box 2 and melanoma inhibitory activity promote oral squamous cell carcinoma progression." Oncotarget. 7(18):26751-26764.
  • Sasahira T, Kurihara M, Nishiguchi Y, Nakashima C, Kirita T, Kuniyasu H. (2016) "Pancreatic adenocarcinoma up-regulated factor has oncogenic functions in oral squamous cell carcinoma." Histopathology. doi: 10.1111/his.13097.
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References:

  • Kessel D, Beck WT, Kukuruga D, Schulz V. (1991) "Characterization of multidrug resistance by fluorescent dyes." Cancer Research 51 17: 4665-4670.
  • Ludescher G, Drach J, Hofmann J, Grunicke H. (1991) "Rapid functional assay for the detection of multidrug-resistant cells using the fluorescent dye rhodamine 123.” Blood 78 5:1385b-1387.
  • Altenberg GA, Vanoye CG, Horton JK, Reuss L. (1994) "Unidirectional fluxes of rhodamine 123 in multidrug-resistant cells: evidence against direct drug extrusion from the plasma membrane." Proceedings of the National Academy of Sciences of the United States of America 91 11: 4654-4657.
  • Wesolowska O, Paprocka M, Kozlak J, Motohashi N, Dus D, Michalak K.(2005) "Human sarcoma cell lines MES-SA and MES-SA/Dx5 as a model for multidrug resistance modulators screening." Anticancer Research 25 1A:383-389.
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