N-(NBD-Aminohexanoyl) sphingosine

Product ID: M0239



Unit SizePriceQuantity 
1mg
$180.95
  • Buy 5 for $144.76 each and save 20%

Availability: In stock


Description

Fluorescent membrane probe. This C6-NBD-ceramide analog is used to detect, at the cellular level, a variety of lysosomal storage diseases as well as intracellular distribution and transport of the labeled sphingolipid molecules in living cells.

Ceramides are the biological building blocks of more complex sphingolipids. Metabolism of ceramides typically occurs in Golgi and endoplasmic reticulum membranes. This fluorescent analog of sphingosine ceramide is incorporated into metabolic pathways and can be used as a vital stain for the Golgi apparatus, endoplasmic reticulum, and nuclear envelope of mammalian cells. In the presence of 10 microM monensin the visibility of the fluorescence is diminished on the plasma membrane, whereas the appearance of the Golgi apparatus is amplified. This probe has been known to produce the two fluorescent metabolites intracellularly, sphingomyelin and glucocerebroside intracellularly in the Golgi apparatus, which can then be transported to the cell surface. Monensin inhibits the ability to remove the fluorescent metabolites sphingomyelin and glucocerebroside from the cell surface by back exchange.

Technical Data
SKU M0239
CAS Number 86701-10-2
Unit Size 1mg
Absorption 460nm
Emission Wavelength 534
Detection Method Fluorescence
Molecular Formula C₃₀H₄₉N₅O₆
Molecular Weight 575.75
Storage Conditions -20C, Desiccated

References and Citations

References:

  • "Molecular cloning and functional analysis of apoxin I, a snake venom-derived apoptosis-inducing factor with L-amino acid oxidase activity." Torii S, Yamane K, Mashima T, Haga N, Yamamoto K, Fox JW, Naito M, Tsuruo T. Biochemistry 39(12): 3197-3205 (2000).
  • "Selective accumulation of the endoplasmic reticulum-golgi intermediate compartment induced by the antitumor drug KRN5500." Kamishohara M, Kenney S, Domergue R, Vistica DT, Sausville EA. Exp. Cell Res. 256(2): 468-479 (2000).
  • "Involvement of sphingosine in mitochondria-dependent Fas-induced apoptosis of type II jurkat T cells." Cuvillier O, Edsall L, Spiegel S. J Biol. Chem. 275: 15691 (2000).
  • "Defining lipid transport pathways in animal cells." Pagano RE, Sleight RG. Science 229: 1051 (1985).
  • "A vital stain for the golgi apparatus." Lipsky NG, Pagano RE. Science 228: 745 (1985).
  • "Intracellular translocation of fluorescent sphingolipids in cultured fibroblasts: endogenously synthesized sphingomyelin and glucocerebroside analogues pass through the golgi apparatus en route to the plasma membrane." Lipsky NG, Pagano RE. J Cell Biol. 100: 27 (1985).
  • "Intracellular processing of lipids: a theory based on studies with fluorescent lipids, liposomes, and cells." Pagano RE. Liposome Letters, A.D. Bangham, Ed., pp. 83-96 (1983).
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