NBD-X, SE

Product ID: M0500



Unit SizePriceQuantity 
25mg
$164.54
  • Buy 5 for $131.63 each and save 21%

Availability: In stock


Description

Lipophilic green fluorescent probe for labeling proteins and antibodies. The X-spacer separates the fluorescent dye from the protein allowing greater sensitivity and reduced perturbation effects on protein activity.

This amine-reactive NBD derivative, containing a convenient succinimidyl ester, can be used to develop environmentally sensitive bioconjugates. NBD is a functional analog of the dinitrophenyl hapten, and its fluorescence is quenched upon binding to anti-dinitrophenyl (anti-DNP) antibodies. The X-spacer separates the fluorescent dye from the protein allowing greater sensitivity and reduced perturbation effects on protein labeling.

Technical Data
SKU M0500
CAS Number 145195-58-0
Unit Size 25mg
Alternative Names NBD-X, Succinimidyl Ester; Succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoate
Absorption 460nm
Emission Wavelength 540
Detection Method Fluorescence
Molecular Formula C₁₆H₁₇O₇N₅
Molecular Weight 391.34
Soluble In DMF, DMSO, dichloromethane
Storage Conditions -20C, Desiccated

References and Citations

Citations:

  • Nussbaumer P, Hornillos V, Ghobrial M, Ullrich T. (2008) "An efficient, one-pot synthesis of various ceramide 1-phosphates from sphingosine 1-phosphate." Chem Phys Lipids. 151(2):125-8.
>Show more
>Show less

References:

  • Eisele F, Kuhlmann J, Waldmann H. (2002) "Synthesis and membrane binding properties of a lipopeptide fragment from influenza virus A hemagglutinin." Chem Eur J 8, 3362 (2002).
  • Karlstrom A, Nygren PK. (2001) "Dual labeling of a binding protein allows for specific fluorescence detection of native protein." Anal Biochem 295, 22-30.
  • Elliott JT, Prestwich GD. (2000) "Maleimide-functionalized lipids that anchor polypeptides to lipid bilayers and membranes." Bioconjug Chem 11, 832-841.
  • Huang TH, Yang DS, Fraser PE, Chakrabartty A. (2000) "Alternate aggregation pathways of the Alzheimer beta -amyloid peptide. An in vitro model of preamyloid." J Biol Chem 275, 36436-36440.
  • Ozaki S, DeWald DB, Shope JC, Chen J, Prestwich GD. (2000) "Intracellular delivery of phosphoinositides and inositol phosphates using polyamine carriers." Proc Natl Acad Sci U S A 97, 11286-11291.
>Show more
>Show less

Click here for Newsletter articles about this product


Technical Support

Question about this product? Ask a Scientist!

We pride ourselves on the high quality of our products and want you to get the best possible results from your assays. If you have any questions about this product or need help optimizing your protocol check out the product FAQs below or ask your own question and one of our expert scientists will get back to you asap:


There are currently no frequently asked questions for this product, click the button above to ask a question