Nitrotetrazolium blue chloride

Product ID: M5277

Unit SizePriceQuantity 
5 g

Availability: In stock


Alkalinephosphatase substrate.

NBT is used in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP) (B-7420, B-7500, B-7505) to detect alkalinephosphatase activity in blotting techniques and in situ hybridization chemistry. BCIP acts as the substrate for alkaline phosphatase, and the oxidizing reagent NBT enhances the purplish-brown final color of the precipitates at the reaction sites. NBT reduction Test: Assessment of reduction of NBT dye by stimulated and unstimulated neutrophils.

Technical Data
SKU M5277
CAS Number 298-83-9
Unit Size 5 g
Alternative Names Nitro blue tetrazolium chloride; NBT; Nitro-TB
Detection Method Chromogenic
Molecular Formula C40H30Cl2N10O6
Molecular Weight 817.65

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