OliGlo™ Cy3 Universal Nucleic Acid Labeling Kit - trial size

Product ID: M1602



Unit SizePriceQuantity 
1kit
$215.42

Availability: In stock


Description

Directly labels the phosphate groups (terminal and backbone) on oligonucleotides, DNA or RNA using a Cy3 labeling reagent prepared in situ, causing less disruption of hybridization and allowing labeling that is not sequence dependent.

This kit when used with the supplied standard labeling protocol will yield labeling efficiency of approximately 10 to 100 labels per kilobase of nucleic acid. The trial size kit is suitable for labeling up to 35 μg of nucleic acid.

Technical Data
SKU M1602
Unit Size 1kit
Detection Method Fluorescence

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References and Citations

References:

  • Brown P, Bostein D. (1999) "Exploring the world of the genome with DNA microarrays." Nat Genet 21: 33–37.
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  • DeRisi JL, van den Hazel B, Marc P, Balzi E, Brown P, Jacq C, Goffequ A. (2000) "Genome microarray analysis of transcriptional activation in multidrug resistance yeast mutants." FEBS Lett 470(2):156-160.
  • Durrant I, Brunning S, Eccleston L, Chadwick P, Cunningham M. (1995) "Fluorescein as a label for non-radioactive in situ hybridization." Histochem J 27:94-99.
  • Forster AC, McInnes JL, Skingle DC, Symons RH. (1985) "Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin." Nucleic Acids Res 13: 745-791.
  • Hoevel T, Kubbies M. (2002) "Nonradioactive labeling and detection of mRNAs hybridized onto nucleic acid cDNA arrays." Methods Mol Biol 185: 417-23.
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  • Olejnik J, Krymanska-Olejnik E, Rothschild KJ. (1998) "Photocleavable aminotag phosphoramidites for 5'-termini DNA/RNA labeling." Nucleic Acids Res 26(15): 3572-3576.
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  • Van Gelder R, von Zastrow ME, Dement WC, Barchas JD, Eberwine JH. (1990) "Amplified RNA synthesized from limited quantities of heterogeneous cDNA." Proc Natl Acad Sci USA 87: 1663–1667.
  • Worley J, Bechtol K, Penn S, Roach D, Hanzel D, Trounstine M, Barker D. (2000) "A systems approach to fabricating and analyzing DNA microarrays." In: Microarray Biochip Technology, edited by Schena M. Natick, MA: Eaton, Chapter. 4, p. 78.
  • Yguerabide J, Talavera E, Alvarez JM, Afkir M. (1996) "Pyrene-labeled DNA probes for homogeneous detection of complementary DNA Sequences: Poly(C) Model System." J Anal Biochem 241: 238-247.
  • Zhou D, Clarke P, Wang J, Chen S. (1996) "Identification of a promoter that controls aromatase expression in human breast cancer and adipose stromal cells." J Biol Chem 271(25): 15194-15202.
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