OliGlo™ TAMRA Universal Nucleic Acid Labeling Kit - standard size

Product ID: M1601

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Availability: In stock


Directly labels the phosphate groups (terminal and backbone) on oligonucleotides, DNA or RNA using a TAMRA labeling reagent prepared in situ, causing less disruption of hybridization and allowing labeling that is not sequence dependent.

A number of agents have been described for labeling nucleic acids to facilitate detection of target DNA or RNA sequences. Suitable labels may provide signals detectable by fluorescence, radioactivity, colorimetry, X-ray diffraction or absorption, magnetism or enzymatic activity. It is essential that the labeling method not perturb base-pairing hybridization critical for preserving assay specificity. Nevertheless, several common methods including labeling by enzymatic incorporation can often lead to interference with the subsequent hybridization detection step, because current fluorescent labels are attached to the base (purine, pyrimidine) portion of the nucleotides where base-pairing and hybridization occurs. In addition, enzymatic labeling methods make use of additional enzymatic steps which require precise calibration to achieve a reproducible labeling yield. Moreover, because the enzymes used depend on the target type (DNA or RNA) and sequence, sequence perturbation is often observed.

To remedy this, methods of direct labeling have been used with varying degrees of success. Direct labeling through phosphodiester and phosphotriester linkages on the DNA or RNA backbone provides the additional advantage of reducing the perturbation of base-pairing hybridization. Our OliGlo™ kits utilize a direct labeling methodology through reaction with the phosphate groups (terminal and backbone) on oligonucleotides, DNA or RNA. The active labeling reagents are prepared in situ from stable precursor molecules derived from TAMRA, allowing the highly reactive labels to function at optimum efficiency for each sample.

The OliGlo™ kits allow molecular biologists and clinical researchers to label or monitor genomic DNA or RNA samples, nucleotides or oligonucleotides for easy detection and quantification. The supplied standard labeling protocol will yield labeling efficiency of approximately 10 labels per kilobase of nucleic acid depending on the purity of the sample. We found that this labeling density is sufficient for most applications. Should there be a need for adjusting labeling efficiency, the end user can simply modify the ratio of labeling dye to nucleic acid as well as incubation times for the labeling reaction as necessary. The Standard kit size provides enough material to label up to 120μg of DNA or RNA.

Technical Data
SKU M1601
Unit Size 1kit
Detection Method Fluorescence

References and Citations


  • Brown P, Bostein D. (1999) "Exploring the world of the genome with DNA microarrays." Nat Genet 21: 33–37.
  • DeRisi JL, Iyer VR, Brown PO. (1997) "Exploring the metabolic and genetic control of gene expression on a genomic scale." Science 278: 680–686.,/li.
  • DeRisi JL, van den Hazel B, Marc P, Balzi E, Brown P, Jacq C, Goffequ A. (2000) "Genome microarray analysis of transcriptional activation in multidrug resistance yeast mutants." FEBS Lett 470(2):156-160.
  • Durrant I, Brunning S, Eccleston L, Chadwick P, Cunningham M. (1995) "Fluorescein as a label for non-radioactive in situ hybridization." Histochem J 27:94-99.
  • Forster AC, McInnes JL, Skingle DC, Symons RH. (1985) "Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin." Nucleic Acids Res 13: 745-791.
  • Hoevel T, Kubbies M. (2002) "Nonradioactive labeling and detection of mRNAs hybridized onto nucleic acid cDNA arrays." Methods Mol Biol 185: 417-23.
  • Kessler C. (1995) "Methods for nonradioactive labeling of nucleic acids." In Nonisotopic probing, blotting, and sequencing (Kricka L J, Ed.) pp 41-109, Academic Press, San Diego.
  • Olejnik J, Krymanska-Olejnik E, Rothschild KJ. (1998) "Photocleavable aminotag phosphoramidites for 5'-termini DNA/RNA labeling." Nucleic Acids Res 26(15): 3572-3576.
  • Rihn H, Coulais C, Bottin MC, Martinet N. (1995) "Evaluation of non-radioactive labelling and detection of deoxyribonucleic acids: Part one: chemiluminescent methods." Biochem Biophys Methods 30:91-102.
  • Sambrook J, Fritsch EF, Maniatis T. (1989) "Molecular Cloning: A Laboratory Manual." 2nd edition.
  • Schena M, Shalon D, Heller R, Chai A, Brown PO, Davis RW. (1996) "Parallel human genome analysis: microarray-based expression analysis of 1000 genes." Proc Natl Acad Sci USA 93: 10614–10619.
  • Stears RL, Getts RC, Gullans SR. (2000) "A novel sensitive detection system for high-density microarrays using dendrimer technology." Physiol Genomics 3:93-99.
  • Takakura M, Kyo S, Kanaya T, Hirano H, Takeda J, Yutsudo M, Inoue M. (1999) "Cloning of human telomerase catalytic subunit (hTERT) gene promoter and identification of proximal core promoter sequences essential for transcriptional activation in immortalized and cancer cells." Cancer Res 59(3) 551-557.
  • van Belkum A, Linkels E, Jelsma T, Houthoff H J, van den Berg F, Quint W. (1993) "Application of a new, universal DNA labeling system in the PCR mediated diagnoses of Chlamydia trachomatis and human papillomavirus type 16 infection in cervical smears." J. Virol. Methods 45: 189-200.
  • Van Gelder R, von Zastrow ME, Dement WC, Barchas JD, Eberwine JH. (1990) "Amplified RNA synthesized from limited quantities of heterogeneous cDNA." Proc Natl Acad Sci USA 87: 1663–1667.
  • Worley J, Bechtol K, Penn S, Roach D, Hanzel D, Trounstine M, Barker D. (2000) "A systems approach to fabricating and analyzing DNA microarrays." In: Microarray Biochip Technology, edited by Schena M. Natick, MA: Eaton, Chapter. 4, p. 78.
  • Yguerabide J, Talavera E, Alvarez JM, Afkir M. (1996) "Pyrene-labeled DNA probes for homogeneous detection of complementary DNA Sequences: Poly(C) Model System." J Anal Biochem 241: 238-247.
  • Zhou D, Clarke P, Wang J, Chen S. (1996) "Identification of a promoter that controls aromatase expression in human breast cancer and adipose stromal cells." J Biol Chem 271(25): 15194-15202.
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