Opti-Klear™ Live Cell Imaging Buffer

Product ID: M1898

Unit SizePriceQuantity 
30mL/5X concentrate
  • Buy 5 for $36.10 each and save 21%

Availability: In stock


HEPES based imaging buffer formulated to lower background fluorescence, maintain proper pH and osmolarity for hours at 37°, provide an energy source and preserve fluorescent signals. Shipped as 5X sterile filtered concentrate.

An ideal substitute for growth media for all extended live cell imaging sessions.

Technical Data
SKU M1898
Unit Size 30mL/5X concentrate
Storage Conditions 2-8C, Protect From Light
Notes “Yes -- we used it. It greatly reduces background -- I have already told a number of colleagues about it. Since it is 5x, a bottle can last for a while.” Dr. Aryeh Weiss, Faculty of Engineering Bar Ilan University, Israel

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References and Citations


  • Abshire JR, Rowlands CJ, Ganesan SM, So PT, Niles JC. (2017) "Quantification of labile heme in live malaria parasites using a genetically encoded biosensor." Proc Natl Acad Sci U S A. pii: 201615195.
  • Kwee E, Powell K, Muschler G (2015) "Characterization of connective tissue progenitors through phase contrast and multicolor fluorescence time-lapse microscopy ", Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 93280O
  • Goldfless SJ, Wagner JC, Niles JC. (2014) "Versatile control of Plasmodium falciparum gene expression with an inducible protein-RNA interaction." Nat Commun. 5:5329.
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  • Diaz G, Isola R,Falchi A, Diana A. (1999) “CO2-Enriched Atmosphere on the Microscope Stage.” BioTechniques 27:292-294.
  • Spierenburg GT, Oerlemans FT, van Laarhoven JP, de Bruyn CH. (1984) “Phototoxicity of N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-buffered culture media for human leukemic cell lines.” Cancer Res. 1984 May;44(5):2253-4.
  • Zigler JS Jr, Lepe-Zuniga JL, Vistica B, Gery I. (1985) “Analysis of the cytotoxic effects of light-exposed HEPES-containing culture medium.” In Vitro Cell Dev Biol. 21(5):282-7.
  • Lepe-Zuniga JL, Zigler JS Jr, Gery I. (1987) “Toxicity of light-exposed Hepes media.” J Immunol Methods. 103(1):145
  • Berthois Y, Katzenellenbogen JA, Katzenellenbogen BS. (1986) “Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture.” Proc Natl Acad Sci U S A. 83(8):2496-500.
  • Lelong IH, Rebel G. (1998) “pH drift of "physiological buffers" and culture media used for cell incubation during in vitro studies.” J Pharmacol Toxicol Methods 39(4):203-10.
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