Opti-Klear™ Live Cell Imaging Buffer with Hoechst 33342/PI (Blue-Red Live:Dead)

Product ID: M2921



Unit SizePriceQuantity 
10mL/1X concentrate
$20.30
  • Buy 5 for $16.24 each and save 20%

Availability: In stock


Description

An ideal substitute for growth media for all extended live cell imaging sessions plus nuclear counterstain and dead cell indicator.

HEPES based imaging buffer formulated to lower background fluorescence, maintain proper pH and osmolarity for hours at 37°, provide an energy source and preserve fluorescent signals. Contains nuclear counterstain Hoechst 33342 and dead cell indicator PI. Shipped as 1X sterile filtered solution.

Technical Data
SKU M2921
Unit Size 10mL/1X concentrate
Storage Conditions 2-8C, Protect From Light

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References and Citations

References:

  • Diaz G, Isola R,Falchi A, Diana A. (1999) “CO2-Enriched Atmosphere on the Microscope Stage.” BioTechniques 27:292-294.
  • Spierenburg GT, Oerlemans FT, van Laarhoven JP, de Bruyn CH. (1984) “Phototoxicity of N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-buffered culture media for human leukemic cell lines.” Cancer Res. 1984 May;44(5):2253-4.
  • Zigler JS Jr, Lepe-Zuniga JL, Vistica B, Gery I. (1985) “Analysis of the cytotoxic effects of light-exposed HEPES-containing culture medium.” In Vitro Cell Dev Biol. 21(5):282-7.
  • Lepe-Zuniga JL, Zigler JS Jr, Gery I. (1987) “Toxicity of light-exposed Hepes media.” J Immunol Methods. 103(1):145
  • Berthois Y, Katzenellenbogen JA, Katzenellenbogen BS. (1986) “Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture.” Proc Natl Acad Sci U S A. 83(8):2496-500.
  • Lelong IH, Rebel G. (1998) “pH drift of "physiological buffers" and culture media used for cell incubation during in vitro studies.” J Pharmacol Toxicol Methods 39(4):203-10.
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