pCambia Vectors

The transformation of plants is common place in hundreds of laboratories worldwide, tranformation is achieved using bacterially-mediated or direct DNA transfer methods. A sizeable technical limitations faced by many labs is that several vectors still used are historical relics with substandard features that make DNA constructions awkward or cumbersome such as: low-copy origin of replication resulting in low yield DNA preps, large size and lack of convenient restriction sites for manipulation.

The pCAMBIA series of vectors was developed by Cambia labs to address many of these issues. The vector backbone is derived from the pPZP vectors and the pCAMBIA vectors offer several advantages over traditional plant vectors:

  • High copy number in E.coli for high DNA yields
  • pVS1 replicon for high stability in Agrobacterium
  • Small size, 7-12kb
  • Restriction sites designed for modular plasmid modifications
  • Small but adequate poly-linkers for introducing your DNA of interest
  • Bacterial selection with chloramphenicol or kanamycin
  • Plant selection with hygromycin B or kanamycin

Marker Gene Technologies, Inc. is pleased to be the exclusive US supplier of the pCambia series of vectors. For further information about the pCambia vectors or help in selecting the appropriate vector for your research needs contact our expert technical assistance department. 

 

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  1. pCambia0105.1R Plant Expression Vector

    pCambia0105.1R Plant Expression Vector

    $134.18

    This plasmid vector carries no resistance gene for plant selection, streptomycin resistance for selection in bacteria and the GusPlus™ reporter gene. Learn More
  2. pCambia0305.1 Plant Expression Vector

    pCambia0305.1 Plant Expression Vector

    $134.18

    Vector contains no plant selection gene, kanamycin resistance gene for bacterial selection and the GusPlus™ reporter gene. Learn More
  3. pCambia0305.2 Plant Expression Vector

    pCambia0305.2 Plant Expression Vector

    $134.18

    Vector contains no plant selection gene, kanamycin resistance gene for bacterial selection, the GusPlus™ reporter gene and the GRP signal peptide for in planta secretion. Learn More
  4. pCambia0380 Plant Expression Vector

    pCambia0380 Plant Expression Vector

    $134.18

    Vector contains no plant selection gene, kanamycin resistance gene for bacterial selection and no reporter gene. Learn More
  5. pCambia0390 Plant Expression Vector

    pCambia0390 Plant Expression Vector

    $134.18

    Vector contains no plant selection gene, kanamycin resistance gene for bacterial selection and no reporter gene. Learn More
  6. pCambia1105.1 Plant Expression Vector

    pCambia1105.1 Plant Expression Vector

    $134.18

    Vector carries hygromycin B resistance gene for plant selection, streptomycin resistance for selection in bacteria, the GusPlus™ reporter gene, and lacZ for white/blue selection. Learn More
  7. pCambia1105.1R Plant Expression Vector

    pCambia1105.1R Plant Expression Vector

    $134.18

    Vector carries hygromycin B resistance gene for plant selection, streptomycin resistance for selection in bacteria and the GusPlus™ reporter gene. Learn More
  8. pCambia1200 Plant Expression Vector

    pCambia1200 Plant Expression Vector

    $134.18

    Vector contains hygromycin B resistance for plant selection, chloramphenicol resistance for bacterial selection and no reporter gene. Learn More

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