pCMVBeta Mammalian lacZ Expression Vector

Product ID: M0951

Unit SizePriceQuantity 

Availability: In stock


High copy number eukaryotic vector, pCMVβ contains the full-length lacZβ-galactosidase gene under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. Very useful for transfection of mammalian cells in culture and for use in other species where constitutive expression is desired.

The β-galactosidase enzyme expression is enhanced by elements including: SD/SA-RNA splice donor and acceptor sequence, and SV40 late polyadenylylation signal. pCMVβ expression vector also contains β-lactamase gene, which acts as a selection marker (100μg/mL ampicillin resistance) in E. coli host. pCMVβ vector has been tested to generate up to 2530u/mg cell extract (MacGregor, and Caskey). In addition, the β-galactosidase gene can be excised using the NotI sites to allow the insertion of other genes to be expressed under the same regulatory elements in mammalian cells. See also our pSV40β vector system, M0952.

Technical Data
SKU M0951
Unit Size 20ug

Full Length Sequence Data

Restriction Sites

References and Citations


  • MacGregor, G.R. & Caskey, C.T., 1989. "Construction of Plasmids that Express E. Coli Beta-galactosidase in Mammalian Cells." Nucleic Acids Res. 17: 2365.
  • Norton, P.A.& Coffin, J.M., 1984. "Bacterial b-galactosidase as a Marker of Rous Sarcoma Virus Gene Expression and Replication." Mol. Cell Biol. 5: 281.
  • Nolan, G.P., et al., 1988. "Fluorescence-activated Cell Analysis and Sorting of Viable Mammalian Cells Based on b-D-galactosidase Activity After Transduction of Escherichia coli lacZ." Proc. Natl. Acad. Sci (USA). 85: 2603.
>Show more
>Show less

Click here for Newsletter articles about this product

Technical Support

Question about this product? Ask a Scientist!

We pride ourselves on the high quality of our products and want you to get the best possible results from your assays. If you have any questions about this product or need help optimizing your protocol check out the product FAQs below or ask your own question and one of our expert scientists will get back to you asap:

There are currently no frequently asked questions for this product, click the button above to ask a question