pCMVBeta Mammalian lacZco Expression Vector

Product ID: M1015



Unit SizePriceQuantity 
20ug
$321.32
  • Buy 25 for $20.56 each and save 94%

Availability: In stock


Description

High copy number eukaryotic vector, pCMVβ expresses the full-length codon-optimized β-galactosidase gene (lacZco) under the control of the cytomegalovirus immediate early gene (CMV IE) promoter.

When expressed in mammalian cells, this codon-optimized gene results in expression levels of β-galactosidase 15-fold higher than those resulting from an analogous construct containing the native E. coli gene sequence. Enhanced transcript stability and increased translational efficiency provide for increased β-galactosidase expression, as suggested by RNA analysis. In addition, codon-optimization results in the elimination of several cryptic splice acceptor sites that are present in the native E. coli gene sequence and increases the amounts of un-spliced, full-length genomic RNA when used in a lentiviral vector containing a 5' splice donor. The β-galactosidase enzyme expression is also enhanced by the SV40 late polyadenylylation signal. This pCMVβ expression vector contains the β-lactamase gene, which acts as a selection marker (100μg/mL ampicillin resistance) in E. coli host. pCMVβ vector has been tested to generate up to 2530u/mg cell extract (MacGregor, and Caskey). If desired, the β-galactosidase codon optimized gene can be excised using XhoI and NotI sites to allow the insertion of other genes to be expressed under the same regulatory elements in mammalian cells. See also our pSV40β lacZco vector system, M1016.

Technical Data
SKU M1015
Unit Size 20ug
Notes

Full Length Sequence Data

Restriction Sites


References and Citations

Citations:

  • Kuroda H, Kutner RH, Bazan NG, Reiser J. (2009) "Simplified lentivirus vector production in protein-free media using polyethylenimine-mediated transfection." J Virol Methods. 157(2):113-21.
  • Kuroda H, Kutner RH, Bazan NG, Reiser J. (2008) "A comparative analysis of constitutive and cell-specific promoters in the adult mouse hippocampus using lentivirus vector-mediated gene transfer. " J Gene Med 2008; 10: 1163–1175.
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References:

  • Anson DS, Limberis M. (2004). "An improved β-galactosidase reporter gene." J. of Biotech. 108: 17-30.
  • MacGregor GR, Caskey CT. (1989) "Construction of plasmids that express E. Coli beta-galactosidase in mammalian cells." Nucleic Acids Res. 17: 2365.
  • Nolan GP, et al. (1988) "Fluorescence-activated cell analysis and sorting of viable mammalian cells based on beta-D-galactosidase activity after transduction of Escherichia coli lacZ." Proc. Natl. Acad. Sci (USA). 85: 2603.
  • Norton PA, Coffin JM. (1984) "Bacterial b-galactosidase as a marker of Rous Sarcoma virus gene expression and replication." Mol. Cell Biol. 5: 281.
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