Peptidase

Peptidases and proteases are important components of cell regulation and signaling, protein activation and synthesis, and other metabolic pathways. Proteases generally cleave longer proteins, while peptidases cleave shorter peptide bonds. Peptidases are further subdivided depending on their hydrolysis sites: endopeptidases cleave internal peptide bonds while exopeptidases hydrolyze terminus residues. 1. Specific amine-containing fluorophores can be conjugated to amino acids and/or proteins through the carboxy terminus, producing fluorogenic peptidase substrates with shorter or non-detectable emission spectra. Upon peptidase activity, the fluorophore is released and the fluorescence restored. 2. Substrate suitability generally depends on enzyme accessibility and the permeability of the substrate and its hydrolyis product.

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  1. in vivo N-AcDEVD-Caspase 3/7 Substrate N

    in vivo N-AcDEVD-Caspase 3/7 Substrate N

    $345.62

    in vivo caspase 3 substrate for intracellular apoptosis detection. Learn More
  2. Propidium iodide

    Propidium iodide

    $67.39

    Excellent counterstain for DNA and chromatin, providing a good contrast when used in combination with green-fluorescent antibodies, or substrates. Learn More
  3. Green FAM Caspase 8 Assay Kit 100tests

    Green FAM Caspase 8 Assay Kit 100tests

    $599.71

    FAM-FLICA® Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. Learn More
  4. Green FAM Caspase 9 Assay Kit 25tests

    Green FAM Caspase 9 Assay Kit 25tests

    $218.44

    FAM-FLICA® Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. Learn More
  5. 7-Amino-4-methylcoumarin

    7-Amino-4-methylcoumarin

    $44.86

    Blue fluorescent reagent useful for preparing aminopeptidase substrates. Learn More
  6. L-Leucine 7-amido-4-methyl coumarin hydrochloride (Leu-AMC.HCl)

    L-Leucine 7-amido-4-methyl coumarin hydrochloride (Leu-AMC.HCl)

    $136.53

    Sensitive fluorogenic substrate for leucine aminopeptidase and aminopeptidase M detection which releases the bright blue fluorescent dye, 7-amino-4-methylcoumarin M1059 upon enzyme activity with emission 440nm upon at excitation 380nm. Learn More
  7. 4-Methylumbelliferyl 4-guanidinobenzoate hydrochloride hydrate

    4-Methylumbelliferyl 4-guanidinobenzoate hydrochloride hydrate

    $22.48

    Spectrofluorometric titrant for chymotrypsin, trypsin, thrombin, and factor Xa. Learn More
  8. EDANS-Sialic Acid Conjugate

    EDANS-Sialic Acid Conjugate

    $51.05

    Reference standard for use with the MarkerGene™ Carbohydrate Analysis Detection Kit M0272. Learn More

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