|
 |
|
Peptidases
and proteases are important components of cell regulation and signaling, protein
activation and synthesis, and other metabolic pathways. Proteases generally cleave longer proteins,
while peptidases cleave shorter peptide bonds. Peptidases are further subdivided depending on their hydrolysis sites:
endopeptidases cleave internal peptide bonds while exopeptidases hydrolyze
terminus residues. 1. Specific
amine-containing fluorophores can be conjugated to amino acids and/or proteins
through the carboxy terminus, producing fluorogenic peptidase substrates with
shorter or non-detectable emission spectra. Upon peptidase activity, the fluorophore is released and the
fluorescence restored. 2. Substrate suitability generally depends on enzyme accessibility and the
permeability of the substrate and its hydrolyis product.
CHEMILUMINESCENT SUBSTRATES
| PRODUCT
# |
PRODUCT
NAME |
WAVELENGTH
OF RELEASED LIGHT |
| M0353 |
N-CBZ-b-Glycinyl-6-Amino-D-Luciferin (for use with a-chymotrypsin
and luciferase, see M0352) |
560nm |
FLUOROGENIC
SUBSTRATES AND KITS
| PRODUCT # |
PRODUCT NAME |
COLOR OF RELEASED PRODUCT
(Abs/Em) (nm) |
| M0520 |
Cyclic
adipoyl-(6-amino)hexanoyl-DEVD-rhodamine 110, triethylamine salt (targets
caspase 3) |
Green
(492/521) |
| M1060 |
L-Leucine-(4-methyl-7-coumarinylamide) hydrochloride (Leu-AMC.HCl) |
Blue (380/440) |
| M0837 M0838 |
Magic
RedTM-(DEVD)2 Caspase 3 Assay Kit (targets caspases 3
and 7) |
Red
(575/620) |
| M0839 M0840 |
Magic
RedTM-(RR)2 Cathepsin B Assay Kit |
Red
(575/620) |
| M0841 M0842 |
Magic
RedTM-(LR)2 Cathepsin K Assay Kit |
Red
(575/620) |
| M0843 M0844 |
Magic
RedTM-(FR)2 Cathepsin L Assay Kit |
Red
(575/620) |
REFERENCES:
1. Methods Enzymol 244, 1 (1994)
2. J Histochem Cytochem 49, 1473 (2001)
| |
