Peroxidase

Reactive oxygen species are a natural byproduct of the normal metabolism of oxygen and have important roles in cell signaling. They have also been implicated in abnormal cellular activity to a variety of inflammatory responses including cardiovascular diseases, immune responses and hearing impairment. Oxidases are also used in a wide variety of bioassays. The most useful known oxidase is horseradish peroxidase (HRP), which has been coupled with sensitive fluorogenic and chromogenic substrates to quantitate intracellular hydrogen peroxide production as well as a diverse assortment of analytes including glucose, galactose, cholesterol, and uric acid. Fluorogenic peroxidase substrates are converted to highly fluorescent products in the presence of the enzyme and hydrogen peroxide but can be relatively unstable for use in enzyme-linked immunosorbent assays (ELISAs). By forming the diacetate derivates, (see M0807) intracellular applications are more efficient, whereupon the acetates are cleaved by endogenous esterases, releasing the intact substrate.

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  1. Methyl purple, sodium salt

    Methyl purple, sodium salt

    $98.80

    Redox indicator, purple to clear upon oxidation. Useful indicator of bacterial growth or plant redox mechanisms. Learn More
  2. Dihydrorhodamine 123

    Dihydrorhodamine 123

    $56.08

    Fluorescent peroxidase substrate. One of the most widely used Reactive Oxygen Species (ROS) probes for intracellular measurement and analysis. Learn More
  3. 2', 7'-Dichlorofluorescin diacetate

    2', 7'-Dichlorofluorescin diacetate

    $59.85

    Reduced derivative of 2',7'-dichlorofluorescein is a cell permeable reagent for use in detection of peroxidase activity (oxidative metabolism, intracellular regulation of reactive oxygen species or oxidative burst) inside living cells. Learn More
  4. pCMVBeta Mammalian lacZ Expression Vector

    pCMVBeta Mammalian lacZ Expression Vector

    $156.40

    High copy number eukaryotic vector, pCMVβ contains the full-length lacZβ-galactosidase gene under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. Very useful for transfection of mammalian cells in culture and for use in other species where constitutive expression is desired. Learn More
  5. pCMVBeta Mammalian lacZco Expression Vector

    pCMVBeta Mammalian lacZco Expression Vector

    $321.32

    High copy number eukaryotic vector, pCMVβ expresses the full-length codon-optimized β-galactosidase gene (lacZco) under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. Learn More
  6. MarkerGene™ Live Cell Fluorescent Reactive Oxygen Species Detection Kit

    MarkerGene™ Live Cell Fluorescent Reactive Oxygen Species Detection Kit

    $247.39

    This kit provides all necessary reagents, buffers and a detailed protocol for the detection of Reactive Oxygen Species (ROS, peroxidase activity) in live cells. Learn More
  7. MarkerGene™ Long Wavelength Fluorescent Lipase Assay Kit

    MarkerGene™ Long Wavelength Fluorescent Lipase Assay Kit

    $280.19

    This kit allows fast and easy measurement of lipase activity in vitro, in cell preparations or in vivo using the long wavelength substrate resorufin oleate. Learn More
  8. Dihydroethidium

    Dihydroethidium

    $95.50

    This reduced form of ethidine shows a blue fluorescence (absorption/emission: 355/420nm) in cell cytoplasm until oxidization to form ethidium which becomes red fluorescent (absorption/emission: 518/605 nm) upon DNA intercalation. Learn More

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