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Reactive oxygen species are a natural byproduct of the normal metabolism of oxygen
and have important roles in cell signaling. They have also been implicated in
abnormal cellular activity to a variety of inflammatory responses including cardiovascular diseases,
immune responses and hearing impairment. Oxidases are also used in a wide variety of bioassays.
The most useful known oxidase is horseradish peroxidase (HRP), which has been coupled with sensitive
fluorogenic and chromogenic substrates to quantitate intracellular hydrogen peroxide production as well as a
diverse assortment of analytes including glucose, galactose, cholesterol, and uric acid. Fluorogenic
peroxidase substrates are converted to highly fluorescent products in the presence of the enzyme and hydrogen
peroxide but can be relatively unstable for use in enzyme-linked immunosorbent assays (ELISAs). By forming
the diacetate derivates, (see M0807) intracellular applications are more efficient, whereupon the acetates are
cleaved by endogenous esterases, releasing the intact substrate.
| ID
| Name
| CAS
| Unit Size
| Price
| Bulk Price (5+) |
| M0121 |
Methyl purple, sodium salt |
209187-17-7 |
500 mg |
80.27 |
64.22 |
| Redox indicator, purple to clear upon oxidation. Useful indicator of bacterial growth or plant redox mechanisms. |
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| M1049 |
MarkerGene™ Live Cell Fluorescent Reactive Oxygen Species Detection Kit |
|
1 kit |
200.97 |
160.78 |
| Provides all necessary reagents, buffers and a detailed protocol for the detection of Reactive Oxygen Species (ROS, peroxidase activity) in live cells. |
|
| M0545 |
Dihydrorhodamine 123 (DHR123) |
109244-58-8 |
2 mg |
45.55 |
36.44 |
| One of the most widely used Reactive Oxygen Species (ROS) probes for intracellular measurement and analysis. |
|
| M1241 |
Dihydroethidium |
104821-25-2 |
10 mg |
52.47 |
41.98 |
| Shows a blue fluorescence (absorption/emission: 355/420nm) in cell cytoplasm until oxidization to form ethidium which becomes red fluorescent (absorption/emission: 518/605 nm) upon DNA intercalation. |
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| M0807 |
2', 7'-Dichlorofluorescin diacetate |
4091-99-0 |
25 mg |
48.62 |
38.89 |
| Reduced derivative of 2',7'-dichlorofluorescein is a cell permeable reagent for use in detection of peroxidase activity (oxidative metabolism, intracellular regulation of reactive oxygen species or oxidative burst) inside living cells. |
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