pH Sensor

Marker Gene Technologies, Inc. offers a selection of dyes useful for cellular or biochemical analysis of pH changes. They include the widely used free-acid fluorescent pH indicator BCECF for cellular and in vitro applications. The AM-ester derivative is membrane permeable and useful for staining live cells. Other offerings in this collection are useful for general labeling of cells, tissues and lysosomes. One key application is the internalization and associated signal change upon pH shift when reactive forms are conjugated to receptors that down regulate their function with internalization and trafficking to acidic compartments, such as the GPCR (G-protein coupled receptor) family.

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  1. 5(6)-Carboxyfluorescein

    5(6)-Carboxyfluorescein

    $29.98

    Extremely useful fluorescent reagent for monitoring intracellular pH and for general labeling of cells and tissues due to its high extinction coefficient, water solubility and pH sensitivity. Learn More
  2. Tetracycline 10-O-β-D-galactopyranoside

    Tetracycline 10-O-β-D-galactopyranoside

    $131.65

    This tetracycline analog useful as an antibiotic with specific release in lacZ transfected cells and tissues. Learn More
  3. 3-Phenylumbelliferone

    3-Phenylumbelliferone

    $44.86

    pH indicator for use in the physiological range (pKa 7.8) Learn More
  4. Pyrene 8-hydroxy-1, 4, 6-trisulfonyl chloride

    Pyrene 8-hydroxy-1, 4, 6-trisulfonyl chloride

    $139.91

    Useful for labeling amine groups of proteins, peptides or other compounds (drugs), and has unusual pH dependent fluorescence properties and pKa changes, after conjugation. Learn More
  5. Sulforhodamine 101 sulfonyl chloride

    Sulforhodamine 101 sulfonyl chloride

    $84.59

    Reactive red fluorophore which couples covalently to amino groups on proteins and other compounds. Suitable for use with FITC in dual parameter flow cytometry studies. Equivalent to Texas Red™. Learn More
  6. 8-Hydroxypyrene-1,3,6-trisulfonic acid trisodium salt

    8-Hydroxypyrene-1,3,6-trisulfonic acid trisodium salt

    $30.50

    Highly water soluble, brightly fluorescent dye useful as a fluorescent pH indicator with emission change in the physiological pH range and as a dye in optical sensor applications. Learn More
  7. 5(6)-Carboxy-2',7'-dichlorofluorescein

    5(6)-Carboxy-2',7'-dichlorofluorescein

    $41.88

    Fluorescein analog with high fluorescence in the low pH range (pH 4-5). Useful for intracellular organelle staining of acidic vesicles. Learn More
  8. 5(6)-Carboxynaphthofluorescein

    5(6)-Carboxynaphthofluorescein

    $59.85

    This pH-dependent fluorophore with red fluorescence (EX 598/ EM 668nm at pH 9) has a high pKa (7.6) value. The fluorescence shifts to shorter wavelengths (EX 512/ EM 567nm) at neutral or acidic pH environments. Learn More

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