Marker Gene Technologies, Inc. offers a selection of dyes useful for cellular or biochemical analysis of pH changes. They include the widely used free-acid fluorescent pH indicator BCECF for cellular and in vitro applications. The AM-ester derivative is membrane permeable and useful for staining live cells. Other offerings in this collection are useful for general labeling of cells, tissues and lysosomes. One key application is the internalization and associated signal change upon pH shift when reactive forms are conjugated to receptors that down regulate their function with internalization and trafficking to acidic compartments, such as the GPCR (G-protein coupled receptor) family.
Extremely useful fluorescent reagent for monitoring intracellular pH and for general labeling of cells and tissues due to its high extinction coefficient, water solubility and pH sensitivity. Learn More
Reactive red fluorophore which couples covalently to amino groups on proteins and other compounds. Suitable for use with FITC in dual parameter flow cytometry studies. Equivalent to Texas Red™. Learn More
This pH-dependent fluorophore with red fluorescence (EX 598/ EM 668nm at pH 9) has a high pKa (7.6) value. The fluorescence shifts to shorter wavelengths (EX 512/ EM 567nm) at neutral or acidic pH environments. Learn More