Phosphatase

Phosphatases utilize a variety of phosphate and polyphosphate esters as substrates, including phospholipids, phophorylated proteins, nucleotides, membrane structural components, and energy reservoirs. Simple phosphatases, such as alkaline and acid phosphatase, hydrolyze phosphate monoesters to an alcohol and inorganic phosphate. Many fluorogenic and chromogenic substrates have been created to measure enzyme activity for these simple phosphate-ester metabolites, as well as conjugates for use as secondary detection reagents in enzyme-linked immunosorbent assays (ELISAs), immunohistochemical techniques, and blot analyzes. Marker Gene is constantly developing new assays and substrates for phosphatase detection. Contact our technical assistance staff for more information about your assay.

5 Item(s)

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  1. N-FMOC Rhodamine 110

    N-FMOC Rhodamine 110

    $82.30

    Useful for the synthesis of heterofunctional R110 derivatives, including peptidase substrates Learn More
  2. 3-Phenylumbelliferone 7-O-phosphate, bis pyridine salt

    3-Phenylumbelliferone 7-O-phosphate, bis pyridine salt

    $89.84

    Alkaline phosphatase substrate which releases the bright green fluorescent dye, 3-phenylumbelliferone, at the site of enzyme activity (EX = 383 nm ; EM = 472 nm). Learn More
  3. Fluorescein diphosphate, tetraammonium salt

    Fluorescein diphosphate, tetraammonium salt

    $269.36

    Ultrasensitive fluorogenic substrate for alkaline phosphatase analyses. Learn More
  4. MarkerGene™ Fluorescent Alkaline Phosphatase Assay Kit

    MarkerGene™ Fluorescent Alkaline Phosphatase Assay Kit

    $304.25

    Allows fast and easy measurement of alkaline phosphatase activity in vitro, in cell preparations or in vivo using the substrate fluorescein diphosphate. Learn More
  5. Dansyl chloride

    Dansyl chloride

    $51.05

    Widely used in protein sequencing and amino acid analysis. Learn More

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