Phosphatases utilize a variety of phosphate and polyphosphate esters as substrates, including phospholipids, phophorylated proteins, nucleotides, membrane structural components, and energy reservoirs. Simple phosphatases, such as alkaline and acid phosphatase, hydrolyze phosphate monoesters to an alcohol and inorganic phosphate. Many fluorogenic and chromogenic substrates have been created to measure enzyme activity for these simple phosphate-ester metabolites, as well as conjugates for use as secondary detection reagents in enzyme-linked immunosorbent assays (ELISAs), immunohistochemical techniques, and blot analyzes. Marker Gene is constantly developing new assays and substrates for phosphatase detection. Contact our technical assistance staff for more information about your assay.