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Phosphatases utilize a variety of phosphate and polyphosphate esters as substrates, including
phospholipids, phophorylated proteins, nucleotides,
membrane structural components, and energy resevoirs. Simple phosphatases, such as alkaline and acid
phosphatase, hydrolyze phosphate monoesters to an alcohol and inorganic phosphate. Many fluorogenic and
chromogenic substrates have been created to measure enzyme activity for these simple phosphate-ester
metabolites, as well as conjugates for use as secondary detection reagents in enzyme-linked immunosorbent
assays (ELISAs), immunohistochemical techniques, and blot anaylses. Marker Gene is constantly developing new assays
and substrates for phosphatase detection. Contact our technical assistance staff for more information about your assay.
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