Enzyme Substrates and Kits for Phosphatase Enzymes.

Phosphatases utilize a variety of phosphate and polyphosphate esters as substrates, including phospholipids, phophorylated proteins, nucleotides, membrane structural components, and energy resevoirs. Simple phosphatases, such as alkaline and acid phosphatase, hydrolyze phosphate monoesters to an alcohol and inorganic phosphate. Many fluorogenic and chromogenic substrates have been created to measure enzyme activity for these simple phosphate-ester metabolites, as well as conjugates for use as secondary detection reagents in enzyme-linked immunosorbent assays (ELISAs), immunohistochemical techniques, and blot anaylses. Marker Gene is constantly developing new assays and substrates for phosphatase detection. Contact our technical assistance staff for more information about your assay.

Product ID Product Name Description
M0541 3-Phenylumbelliferone 7-O-phosphate hemipyridinium salt  Alkaline phosphatase substrate which releases the bright green fluorescent dye, 3-phenylumbelliferone, at the site of enzyme activity (EX = 383 nm ; EM = 472 nm). 
M1034 Fluorescein diphosphate, tetraammonium salt  The most widely used ultrasensitive fluorogenic substrate for alkaline phosphatases. 
M1207 Resorufin-7-O-phosphate, diammonium salt (Res-Phos)  This new long-wavelength fluorescent substrate is useful in a variety of important phosphatase analyses. The ResPhosTM phosphatase substrate, (Ex/Em=571/583nm upon dephosphorylation), can be used to quantify phosphatase activity in solutions, in cell extracts, in live cells as well as on solid surfaces (such as PVDF membranes). It complements our existing products for phosphatase analysis, including the substrates 3-Phenylumbelliferone 7-O-phosphate, hemipyridinium salt Product M0541 and Fluorescein diphosphate, tetraammonium salt Product M1034. 
M1222 MarkerGeneTMFluorescent Alkaline Phosphatase Assay Kit  The Fluorescent Alkaline Phosphatase Assay Kit allows fast and easy measurement of alkaline phosphatase activity in vitro, in cell preparations or in vivo using the substrate fluorescein diphosphate. 
     
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