Product M0011
5(6)-Carboxyfluorescein diacetate (CFDA)
(High Purity-Molecular Biology Grade)


5(6)-Carboxyfluorescein diacetate (CFDA)

Molecular Weight: 460.4

Storage: F,D

Soluble: DMSO

Absorption: (in nm) 300

Extinction (103): 63

Molecular Formula: C25H16O9

CAS Number: [5 isomer [79955-27-4]; 6 isomer [3348-03-6]]

Alternative Name: High Purity-Molecular Biology Grade

Unit Price (USD): 100 mg $108.50

Bulk Price/unit (when you buy 5 or more): $86.80

Description:
CFDA is a popular, ultrasensitive, fluorescent substrate for measuring esterase activity in live cells. It is often used in cell viability assays and "Live-Dead" assay systems, especially automated cell viability assays, because the cleavage product produced upon enzymatic or chemical hydrolysis of the acetate group(s) is carboxy fluorescein (Product M0122). This fluorophore is well-retained inside live cells, while the substrate CFDA, is membrane permeant. See the references for additional information about CFDA use in bacterial, plant, yeast and mammalian cell culture.

Application:
Cell labeling reagent with green emission spectral properties (Abs 488 nm / EM 517 nm) after removal of acetate esters, intracellularly.

References:

  • "A trace amount of the human pro-apoptotic factor bax induces bacterial death accompanied by damage of DNA." Asoh S, Nishimaki K, Nanbu-Wakao R., Ohta S. J Biol. Chem. 273(18): 11384-91 (1998).
  • "Subcellular compartmentation of different lipophilic fluorescein derivatives in maize root epidermal cells." Brauer D., et al. Protoplasma 192: 70 (1996).
  • "Role of three Rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the endocytic and vacuolar protein sorting pathways of yeast." Singer-Kruger B, Stenmark H, Dusterhoft A, Philippsen P, Yoo JS, Gallwitz D, Zerial M. J Cell. Biol. 125(2): 283-298 (1994).
  • "Measurement of intracellular fluorescence of human monocytes relative to oxidative metabolism." Robinson JP, Bruner LH, Bassoe CF, Hudson JL, Ward PA, Phan SH. J Leukocyte Biol. 43(4): 304-310 (1988).

    •


    Keywords:

    Carboxyfluorescein diacetate, Carboxyfluorescein, diacetate, fluorescein, M0011, CFDA, 79955-27-4, 3348-03-6, Marker Gene