Absorption: (in nm) 330

Emission: (in nm) 396

Extinction (10³): 16

Molecular Formula: C₁₆H₁₆O₁₀

CAS Number: [64664-99-9]

Alternative Name: High Purity-Molecular Biology Grade

Unit Price (USD): 5 mg $108.50

Bulk Price/unit (when you buy 5 or more): $86.80

Description:
Blue fluorogenic β-galactosidase substrate that is well retained intracellularly. The fluorescence spectrum for the released 3-carboxyumbelliferone is pH-dependent. Below the pKa (7.8), Abs. shifts to shorter wavelengths (325 to 340 nm) and fluorescence intensity decreases. These shifts may be used to measure compartmentalization of intracellular activity.

Note:
Em = 445nm, ε = 32K is emission of fluorophore 3-carboxyumbelliferone released upon β-Galactosidase enzyme activity.

References:

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• "Homogeneous substrate-labeled fluorescent immunoassay for carbamazepine." Li TM, Miller JE, Ward FE, Burd JF. Epilepsia 23: 391-398 (1982).

• "Serum theophylline determination by fluorescence polarization immunoassay utilizing an umbelliferone derivative as a fluorescent label." Li TM, Benovic JL, Burd JF. Anal Biochem 118: 102-107 (1981).

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• "Substrate-labeled homogeneous fluorescent immunoassay for tobramycin, ASEAN." Boguslaski RC, Burd JF, Denning CE. J. Clin. Sci. 1(3): 215-19 (1980).

• "Substrate-labeled fluorescent immunoassay for phenobarbital." Krausz LM, Hitz JB, Buckler RT, Burd JF. Ther. Drug Monit. 2(3): 261-72 (1980).