Molecular Formula: N/A
CAS Number: [N/A]
Unit Price (USD):
1 kit $101.50
Bulk Price/unit (when you buy 5 or more): $81.20
Description:
This kit contains synthetic D-luciferin, the primary substrate of the firefly luciferase light producing system, as well as a luciferase assay buffer (10 mL, for a 1 mM reagent concentration), and other reagents, enough to perform 1000 assays using a microtiterplate format (100 uL reaction volume, 0.1 mM final reaction concentration).
The synthetic beetle luciferin substrate contained in the kit is also available separately as a lyophilized powder D-Luciferin, Product M0237. Consult the Product information sheet (above) for more details on the assay protocol.
References:
"Transmembrane Motility Assay of Transiently Transfected Cells by
Fluorescent Cell Counting and Luciferase Measurement." J.J. Gildea, et al.
Biotechniques 29: 81 (2000).
"The Use of the Luciferase Reporter System in Plant Gene Expression
Studies." W. Van Leeuwen, et al. Plant Mol. Biol. Rep. 18:143a (2000).
"Reporter Genes and Transient Assays for Plants." B.F. Matthews, et al.
Methods Mol. Biol. 55:147 (1995)
"Firefly Luciferase, Synthesized to Very High Levels in Caterpillars Infected
with a Recombinant Baculovirus, Can Also Be Used As an Effective
Reporter Enzyme In Vivo." P.K. Jha, et al. FEBS Lett. 274: 23 (1990)
