Molecular Weight: 712.61

Storage: F, D
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Soluble: H₂O, DMSO, MeOH

Absorption: (in nm) 300

Emission: (in nm) 514

Molecular Formula: C₃₄H₃₂O₁₇

CAS Number: [N/A]

Unit Price (USD): 5 mg $101.86

Bulk Price/unit (when you buy 5 or more): $81.49

Description:
Sensitive cell-permeant beta-glucuronidase substrate for live cell and tissue (in vivo and in vitro) assays.

Note:
Emission of the fluorescein fluorophore (EX 488nm / EM 512nm) released upon beta-glucuronidase enzyme activity.

References:

• Lorincz M, Roederer M, Diwu Z, Herzenberg LA, Nolan GP. "Enzyme-generated intracellular fluorescence for single-cell reporter gene analysis utilizing Escherichia coli beta-glucuronidase." Cytometry 24, 321-329 (1996).
• Lorincz MC, Parente MK, Roederer M, Nolan GP, Diwu Z, Martin DI, Herzenberg LA, Wolfe JH. "Single cell analysis and selection of living retrovirus vector-corrected mucopolysaccharidosis VII cells using a fluorescence-activated cell sorting-based assay for mammalian beta-glucuronidase enzymatic activity." J Biol Chem 274, 657-665 (1999).
• Naleway, JJ., in "GUS Protocols: Using the GUS Gene as a Reporter of Gene Expression", S.R.Gallagher, Ed., Acad. Press, 61-76 (1992).