Molecular Formula: N/A

CAS Number: [N/A]

Unit Price (USD): 1 kit $191.40

Bulk Price/unit (when you buy 5 or more): $153.12

Description:
The Live Cell Fluorescent Reactive Oxygen Species Detection Kit provides all necessary reagents, buffers and a detailed protocol for the detection of Reacive Oxygen Species (ROS, peroxidase activity) in live cells. The assay utilizes the cell permeable reagent, 2,7-dichlorofluorescin diacetate Product M0807 , a reliable fluorogenic marker for ROS detection. Upon enzyme activity, the highly fluorescent dye, 2,7-dichlorofluorescein is produced, with EX: 495nm and EM: 529 nm.

Application:
Fluorogenic peroxidase substrates are converted to highly fluorescent products in the presence of the enzyme and hydrogen peroxide but can be relatively unstable for use in enzyme-linked immunosorbent assays (ELISAs). By forming the diacetate derivates, intracellular applications are more efficient, whereupon the acetates are cleaved by endogenous esterases, releasing the intact substrate.

References:

• "Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes." Diaz G, Liu S, Isola R, Diana A, Falchi AM. Histochem. Cell Biol. 120: 319-25 (2003).
• "Oxidative stress-induced iron signaling is responsible for peroxide-dependent oxidation of dichlorodihydrofluorescein in endothelial cells: role of transferrin receptor-dependent iron uptake in apoptosis." Tampo Y, Kotamraju S, Chitambar CR, Kalivendi SV, Keszler A, Joseph J, Kalyanaraman B. Circ. Res. 92: 56-63 (2003).
• "Screening assays for the mitochondrial permeability transition using a fluorescence multiwell plate reader." Blattner JR, He L, Lemasters JJ. Anal. Biochem. 295: 220-226 (2001).
• "The use of fluorescent probes to assess oxidative processes in isolated-perfused rat heart tissue." Kehrer JP, Paraidathathu T. Free Radic. Res. Commun. 16: 217-225 (1992).
• "Visualization of hydrogen peroxide in living polymorphonuclear neutrophils utilizing leucodiacetyl 2,7-dichlorofluorescin: photomicrographic and microphotometric studies." Salata RA, Sullivan JA, Mandell GL. Trans. Assoc. Am. Physicians 96: 375-383 (1983).
• "The fluorometric analysis of ultramicro quantities of hydrogen peroxide." Keston AS, Brandt R. Anal. Biochem. 11: 1-5 (1965).