Molecular Weight: 358.35

Storage: F, D

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Soluble: DMF, DMSO, Acetonitrile

Absorption: (in nm) 430

Emission: (in nm) 470

Extinction (10³): 56

Molecular Formula: C₁₈H₁₈O₆N₂

CAS Number: [139346-57-9]

Alternative Name: N-Succinimidyl 7-(diethylamino)coumarin-3-carboxylate

Unit Price (USD): 25 mg $76.45

Bulk Price/unit (when you buy 5 or more): $61.16

Description:
This amine reactive fluorescent probe is useful for labeling amino acids prior to resolution by capillary zone electrophoresis and for multicolor DNA and protein labeling experiments.

References:

• "Fluorescent coumarin-labeled nucleotides to measure adp release from actomyosin." Webb MR, Corrie JE. Biophys. J. 81: 1562-1569 (2001).
• "Protein-free parallel triple-stranded DNA complex formation." Shchyolkina AK, Timofeev EN, Lysov YP, Florentiev VL, Jovin TM, Arndt-Jovin DJ. Nucleic Acids Res. 29: 986-995 (2001).
• "New strategy for multi-colour fluorescence in situ hybridisation: COBRA: COmbined Binary RAtio labelling." Tanke HJ, Wiegant J, van Gijlswijk RP, Bezrookove V, Pattenier H, Heetebrij RJ, Talman EG, Raap AK, Vrolijk J. Eur. J. Hum. Genet. 7: 2-11 (1999).
• "Cooperative formation of a substrate binding pocket by alpha- and beta-subunits of mitochondrial processing peptidase." Kojima K, Kitada S, Shimokata K, Ogishima T, Ito A. J Biol. Chem. 273: 32542-32546 (1998).
• "Glutamate residues required for substrate binding and cleavage activity in mitochondrial processing peptidase." Kitada S, Kojima K, Shimokata K, Ogishima T, Ito A. J Biol. Chem. 273: 32547-32553 (1998).
• "Highly sensitive detection using laser two-photon excited fluorescence in capillary electrophoresis." Song JM, et al. J Chromatogr. A. 765: 315 (1997).
• "Determination of amino acids by capillary zone electrophoresis based on semiconductor laser fluorescence detection." Higashijima T. et al. Anal. Chem. 64: 711 (1992).