Molecular Weight: 67000

Storage: F, D, L

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Soluble: H₂O, aqueous buffers

Absorption: (in nm) 595

Emission: (in nm) 615

CAS Number: [N/A]

Alternative Name: Texas Red ™ Avidin

Unit Price (USD): 5 mg $136.07

Bulk Price/unit (when you buy 5 or more): $108.86

Description:
Fluorescein, Texas Red^TM as well as TAMRA conjugates of Avidin and Streptavidin have become increasingly useful for DNA and RNA microarray analyses, as a method of detecting biotin-labeled antibodies, or in ELISA assays. Marker Gene's Texas Red^TM Avidin is a highly fluorescent conjugate of Avidin and sulforhodamine 101. This red fluorescent protein excites at 595 nm with an emission maximum at 615 nm. Since the excitation and emission maxima are well separated from those of fluorescein, Texas Red^TM Avidin can be employed with other fluorescein or coumarin conjugates to simultaneously localize two or three antigens in the same tissue section. Texas Red^TM Avidin is ideal for flow cytometry applications using instruments equipped with dye lasers. The great affinity of avidin for D-biotin (Ka = 10-15/M) provides for a great number of applications in biochemistry (immunoassays, receptor and histochemical studies, bacteriophage inhibitions). This fluorescently labeled avidin conjugate combines stoichiometrically with biotin, having 4-biotin binding sites per avidin molecule. In addition, the Texas Red^TM Avidin is a highly cationic glycoprotein that can selectively bind to a component in human and murine mast cell granules in fixed-cell preparations, and can thus be used to identify mast cells in normal and diseased human tissue without requiring a biotinylated probe. See also our Fluorescein Isothiocyanate (FITC) Avidin Conjugate, Product M1274.

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