MR-DEVD2 Apoptosis Detection Kits

Magic Red™ products are substrate-based assays designed to detect protease activity within whole living cells, using a fluroescence microscope. Magic Red™ assays are based on a the cresyl violet leaving group that fluoresces once the enzyme specific peptides are cleaved. No wash steps are required, making these kits very user friendly. In these kits, Magic Red™ is covalently attached to a specific peptide (DEVD) that when cleaved, causes the Magic Red™ to fluoresce. Assay kits are available in 25-test and 100-test sizes.  





These Magic Red™ substrate-based assays are designed to detect Caspase protease activity within whole living cells, using fluorescence microscopy.  Magic Red™ assays are based on the cresyl violet leaving group that fluoresces upon enzyme specific peptidase activity. 

 

Product

number

Magic Red™

Peptide

Target

Caspase(s)

Product Name

# Tests

Price

M0837

MR-(DEVD)2

Caspase 3 and 7

Magic Red™-(DEVD)2  Caspase 3  Assay Kit

25

$145.95

M0838

MR-(DEVD)2

Caspase 3 and 7

Magic Red™-(DEVD)2  Caspase 3 Assay Kit

100

$345.45

References:

  • Arends, M.J., Wylie, A.H., (1991) "Apoptosis: Mechanisms and roles in pathology." Int. Rev. Exp. Pathol. 32:223-254.

  • Slee, E.A., Adrian, C., Martin, S.J., (1999) "Serial Kollers: Ordering Caspase Activation events in Apoptosis", Cell Death and Differ. 6: 1067-1074

  • Salvesen, G.S., Dixit, V.M., (1997) "Caspases: intracellular signaling by proteoplysis", Cell 91:443-446.

  • Rotonda, J., D. W. Nicholson, K. M. Fazil, M. Gallant, Y. Gareau, M. Labelle, E. P. Peterson, D. M. Rasper, R. Ruel, J. P. Vaillancourt, N. A. Thornberry and J. W. Becker. 1996. The three- dimensional structure of apopain/ CPP32, a key mediator of apoptosis. Nature Struct. Biol. 3( 7): 619- 625.

  • Kumar, S. 1999. Mechanisms mediating caspase activation in cell death. Cell Death and Differ. 6: 1060- 1066.

  • Thornberry, N. A., T. A. Rano, E. P. Peterson, D. M. Rasper, T. Timkey, M. Garcia- Calvo, V. M. Houtszager, P. A. Nordstrom, S. Roy, J. P. Vaillancourt, K. T. Chapman and D. W. Nicholson. 1997. A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis. J. Biol. Chem. 272( 29): 17907- 17911.

  • Ekert, P. G., J. Silke and D. L. Vaux. 1999. Caspase inhibitors. Cell Death and Differ. 6: 1081- 1086.

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