| ID
| Name
| CAS
| Unit Size
| Price
| Bulk Price (5+) |
| M1713 |
SophiaGreen™ T-1 |
|
200ul 1mM Solution |
310.00 |
248.00 |
| Excellent counterstain for DNA and chromatin, providing high increase in fluorescence upon binding to nucleic acid |
|
| M1714 |
SophiaGreen™ T-2 |
|
200ul 1mM Solution |
325.00 |
260.00 |
| Excellent counterstain for DNA and chromatin, providing high increase in fluorescence upon binding to nucleic acid. |
|
| M1716 |
SophiaGreen™ Y-1 |
|
200ul 1mM Solution |
325.00 |
260.00 |
| Excellent counterstain for DNA and chromatin, providing high increase in fluorescence upon binding to nucleic acid. |
|
| M1416 |
2', 7'-Dichlorofluorescein di-Β-D-lactoside |
|
5mg |
106.95 |
85.56 |
| Highly sensitive fluorescent substrate for measuring Β-lactosidase or galactosidase and glucosidase activity inside of live cells and lysosomes. |
|
| M0710 |
In vivo N-AcDEVD- Caspase 3/7 Substrate N |
|
500ug |
280.78 |
224.62 |
| A new in vivo caspase 3 substrate for intracellular apoptosis detection. |
|
| M1581 |
GusPlus™ No Plant Selection/Kanamycin Resistant Plant Expression Vector (pCambia0305.1) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC18 poly-linker for introducing your DNA of interest. Contains no plant selection gene and kanamycin resistance gene for bacterial selection. Contains the GusPlus&trade reporter gene.
|
|
| M1579 |
GusPlus™ No Plant Selection/Streptomycin Resistant Plant Expression Vector (pCambia0105.1R) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC18 poly-linker for introducing your DNA of interest. Carries no resistance gene for plant selection, streptomycin resistance for selection in bacteria and the GusPlus™ reporter gene.
|
|
| M1582 |
Secreted GusPlus™No Plant Selection/Kanamycin Resistant Plant Expression Vector (pCambia0305.2) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC18 poly-linker for introducing your DNA of interest. Contains no plant selection gene and kanamycin resistance gene for bacterial selection. Contains the GusPlus™ reporter gene and the GRP signal peptide for in planta secretion.
|
|
| M1583 |
No Reporter, No Plant Selection/Kanamycin Resistant pUC8 Plant Expression Vector (pCambia0380) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC8 poly-linker for introducing your DNA of interest. Contains no plant selection gene, kanamycin resistance gene for bacterial selection and no reporter gene.
|
|
| M1584 |
No Reporter, No Plant Selection/Kanamycin Resistant pUC9 Plant Expression Vector (pCambia0390) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC9 poly-linker for introducing your DNA of interest. Contains no plant selection gene, kanamycin resistance gene for bacterial selection and no reporter gene.
|
|
| M1586 |
GusPlus™/lacZ Hygromycin B/Streptomycin Resistant Plant Expression Vector (pCambia1105.1) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC18 poly-linker for introducing your DNA of interest. Carries hygromycin B resistance gene for plant selection, streptomycin resistance for selection in bacteria and the GusPlus™ reporter gene. Also carries lacZ for white/blue selection.
|
|
| M1587 |
GusPlus™ Hygromycin B/Streptomycin Resistant Plant Expression Vector (pCambia1105.1R) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC18 poly-linker for introducing your DNA of interest. Carries hygromycin B resistance gene for plant selection, streptomycin resistance for selection in bacteria and the GusPlus™ reporter gene.
|
|
| M1588 |
No Reporter Gene Hygromycin B/Chloramphenicol Resistant Plant Expression Vector (pCambia1200) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC18 poly-linker for introducing your DNA of interest. Contains hygromycin B resistance for plant selection, chloramphenicol resistance for bacterial selection and no reporter gene.
|
|
| M1589 |
gusA Hygromycin B/Chloramphenicol Resistant Plant Expression Vector (pCambia1201) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC18 poly-linker for introducing your DNA of interest. Contains hygromycin B resistance for plant selection, chloramphenicol resistance for bacterial selection and gusA reporter gene.
|
|
| M1590 |
gusA/lacZ Hygromycin B/Chloramphenicol Resistant Plant Expression Vector (pCambia1281Z) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC8 poly-linker for introducing your DNA of interest. Contains hygromycin B resistance for plant selection, chloramphenicol resistance for bacterial selection and gusA reporter gene along with lacZ for white/blue selection.
|
|
| M1591 |
No Reporter Gene Hygromycin B/Kanamycin Resistant Plant Expression Vector (pCambia1300) |
|
20ug |
109.00 |
|
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC18 poly-linker for introducing your DNA of interest. Contains kanamycin resistance gene for bacterial selection and hygromycin B resistance gene for plant selection. Contains no reporter gene to allow construction of users own system.
|
|
| M1592 |
gusA Hygromycin B/Kanamycin Resistant Plant Expression Vector (pCambia1301) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC18 poly-linker for introducing your DNA of interest. Contains kanamycin resistance gene for bacterial selection and hygromycin B resistance gene for plant selection. Also incorporates the gusA reporter gene.
|
|
| M1593 |
mgfp5 Hygromycin B/Kanamycin Resistant Plant Expression Vector (pCambia1302) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC18 poly-linker for introducing your DNA of interest. Contains kanamycin resistance gene for bacterial selection and hygromycin B resistance gene for plant selection. Also incorporates the mgfp5 reporter gene.
|
|
| M1594 |
mgfp5:gusA Hygromycin B/Kanamycin Resistant Plant Expression Vector (pCambia1303) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC18 poly-linker for introducing your DNA of interest. Contains kanamycin resistance gene for bacterial selection and hygromycin B resistance gene for plant selection. Also incorporates the gusA:mgfp5 fusion reporter genes.
|
|
| M1595 |
gusA:mgfp5 Hygromycin B/Kanamycin Resistant Plant Expression Vector (pCambia1304) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC18 poly-linker for introducing your DNA of interest. Contains kanamycin resistance gene for bacterial selection and hygromycin B resistance gene for plant selection. Also incorporates the mgfp5:gusA fusion reporter genes.
|
|
| M1596 |
GusPlus™ Hygromycin B/Kanamycin Resistant Plant Expression Vector (pCambia1305.1) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC18 poly-linker for introducing your DNA of interest. Contains kanamycin resistance gene for bacterial selection and hygromycin B resistance gene for plant selection. Also incorporates the GusPlus™ reporter gene.
|
|
| M1597 |
Secretory GusPlus™ Hygromycin B/Kanamycin Resistant Plant Expression Vector (pCambia1305.2) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC18 poly-linker for introducing your DNA of interest. Contains kanamycin resistance gene for bacterial selection and hygromycin B resistance gene for plant selection. Also incorporates the GusPlus™ reporter gene and the GRP signal peptide for in planta secretion.
|
|
| M1598 |
No Reporter Hygromycin B/Kanamycin Resistant pUC8 Plant Expression Vector (pCambia1380) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC8 poly-linker for introducing your DNA of interest. Contains kanamycin resistance gene for bacterial selection and hygromycin B resistance gene for plant selection. Contains no reporter gene to allow construction of user's own system.
|
|
| M1599 |
Fusible gusA (ORF a) Hygromycin B/Kanamycin Resistant pUC8 Plant Expression Vector (pCambia1381Xa) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC8 poly-linker for introducing your DNA of interest. Contains kanamycin resistance gene for bacterial selection and hygromycin B resistance gene for plant selection. Also incorporates the gusA reporter gene with no start codon to allow fusion of your gene to gusA with reading frame a.
|
|
| M1700 |
FusiblegusA (ORF b) Hygromycin B/Kanamycin Resistant pUC8 Plant Expression Vector (pCambia1381Xb) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC8 poly-linker for introducing your DNA of interest. Contains kanamycin resistance gene for bacterial selection and hygromycin B resistance gene for plant selection. Also incorporates the gusA reporter gene with no start codon to allow fusion of your gene to gusA with reading frame b.
|
|
| M1701 |
Fusible gusA (ORF c) Hygromycin B/Kanamycin Resistant pUC8 Plant Expression Vector (pCambia1381Xc) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC8 poly-linker for introducing your DNA of interest. Contains kanamycin resistance gene for bacterial selection and hygromycin B resistance gene for plant selection. Also incorporates the gusA reporter gene with no start codon to allow fusion of your gene to gusA with reading frame c.
|
|
| M1702 |
gusA/lacZ Hygromycin B/Kanamycin Resistant pUC8 Plant Expression Vector (pCambia1381Z) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC8 poly-linker for introducing your DNA of interest. Contains kanamycin resistance gene for bacterial selection and hygromycin B resistance gene for plant selection. Also incorporates the gusA reporter gene and lacZ for white/blue selection.
|
|
| M1703 |
No Reporter Gene Hygromycin B/Kanamycin Resistant pUC9 Plant Expression Vector (pCambia1390) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC9 poly-linker for introducing your DNA of interest. Contains kanamycin resistance gene for bacterial selection and hygromycin B resistance gene for plant selection. Contains no reporter gene to allow construction of user's own system.
|
|
| M1704 |
gusA Hygromycin B/Kanamycin Resistant pUC9 Plant Expression Vector (pCambia1391) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC9 poly-linker for introducing your DNA of interest. Contains kanamycin resistance gene for bacterial selection and hygromycin B resistance gene for plant selection. Also incorporates the gusA reporter gene.
|
|
| M1705 |
Fusible gusA (ORF a) Hygromycin B/Kanamycin Resistant pUC9 Plant Expression Vector (pCambia1391Xa) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC9 poly-linker for introducing your DNA of interest. Contains kanamycin resistance gene for bacterial selection and hygromycin B resistance gene for plant selection. Also incorporates the gusA reporter gene with no start codon to allow fusion of your gene to gusA with reading frame a.
|
|
| M1706 |
Fusible gusA (ORF c) Hygromycin B/Kanamycin Resistant pUC9 Plant Expression Vector (pCambia1391Xc) |
|
20 ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC9 poly-linker for introducing your DNA of interest. Contains kanamycin resistance gene for bacterial selection and hygromycin B resistance gene for plant selection. Also incorporates the gusA reporter gene with no start codon to allow fusion of your gene to gusA with reading frame c.
|
|
| M1707 |
gusA/lacZ Hygromycin B/Kanamycin Resistant pUC9 Plant Expression Vector (pCambia1391Z) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC8 poly-linker for introducing your DNA of interest. Contains kanamycin resistance gene for bacterial selection and hygromycin B resistance gene for plant selection. Also incorporates the gusA reporter gene and the lacZ gene for white/blue selection.
|
|
| M1708 |
No Reporter Gene Kanamycin/Chloramphenicol Resistant Plant Expression Vector (pCambia2200) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC18 poly-linker for introducing your DNA of interest. Contains kanamycin resistance for plant selection, chloramphenicol resistance for bacterial selection and no reporter gene.
|
|
| M1709 |
No Reporter Gene Kanamycin/Kanamycin Resistant Plant Expression Vector (pCambia2300) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC18 poly-linker for introducing your DNA of interest. Contains genes for bacterial and plant selection with kanamycin. Contains no reporter gene to allow construction of user's own system.
|
|
| M1710 |
gusA Kanamycin/Kanamycin Resistant Plant Expression Vector (pCambia2301) |
|
20ug |
109.00 |
109.00 |
| A high copy number plasmid in E.coli for high DNA yields with the pVS1 replicon for high stability in Agrobacterium all contained in a relatively small size of vector. Contains restriction sites designed for modular plasmid modifications and pUC18 poly-linker for introducing your DNA of interest. Contains genes for bacterial and plant selection with kanamycin and the gusA reporter gene.
|
|
| M1745 |
MarkerGene™ Fluorescent No-Wash Cell Count Normalization Kit |
|
|
115.00 |
92.00 |
| Medium to high-throughput cell-based assays often require well-to-well normalization in order to quantitate the data generated. In most cases, this normalization requires knowledge of the cell density, or relative number of cells per well. The cell density has been represented by a variety of methods including manual counting using a hemacytometer with or without Trypan blue staining, as well as measuring the DNA content of the cells. In this kit, the ultrasensitive DNA binding bis-benzamide dye is used to stain the nuclear DNA. As opposed to other methods this staining does not require lysis of the cells so can be used in parallel with the main assay with few additional steps. |
|
| M1585 |
MarkerGene™ Total Protein Determination Kit |
|
|
85.00 |
|
| The ability to quantify the amount of protein in a sample is vital to almost all aspects of research. This quantification should be both quick and easy to perform. To this end the MarkerGene™ Total Protein Determination Kit which utilizes the principles of the Bradford Assay for protein quantification is very useful, allowing the amount of protein in a sample to be deduced in a very short time. |
|
| M1796 |
pFHluc Mammalian Luciferase Expression Vector with Cloning Site |
|
20μg |
250.00 |
|
| High copy number eukaryotic vector, pFHluc, expresses a mutant luciferase gene under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. Contains a multiple cloning site with 11 unique restriction sites. This vector encodes a mutant enzyme that catalyzes the production of long wavelength (red) light (Em:619) from D-luciferin, M0237. |
|
| M1092 |
N-Acetyl-D-glucosaminitol |
4271-28-7 |
1 g |
89.40 |
71.52 |
| Carbohydrate analog useful in differentiation of bacteria. |
|
| M1059 |
7-Amino-4-methylcoumarin |
26093-31-2 |
100 mg |
36.44 |
29.15 |
| Blue fluorescent reagent useful for preparing aminopeptidase substrates. |
|
| M0352 |
6-Amino-D-luciferin |
118969-27-0 |
5 mg |
133.75 |
107.00 |
| Amino analog of D-luciferin substrate for firefly luciferase activity. |
|
| M0084 |
5-(2-Aminoethylamino)- 1-naphthalenesulfonic acid, sodium salt (1,5-EDANS, Na+salt) |
100900-07-0 |
250 mg |
45.55 |
36.44 |
| Water soluble blue-green fluorescent labeling reagent for modification of proteins and carbohydrates. |
|
| M0273 |
5-(2-Aminoethylamino)-1-naphthalenesulfonic acid (1,5-EDANS) |
50402-56-7 |
10 mg |
36.44 |
29.15 |
| Blue-green fluorescent labeling reagent for carbohydrates, aldehydes, ketones and proteins. |
|
| M0845 |
MitoPT™ Apoptosis Detection Kit 100tests |
N/A |
1 kit |
182.70 |
182.70 |
| Use of the MitoPT™ kit allows the easy distinction between non-apoptotic red fluorescent cells and apoptotic green fluorescent cells. |
|
| M0846 |
MitoPT™ Apoptosis Detection Kit 400tests |
N/A |
1 kit |
350.70 |
350.70 |
| Use of the MitoPT™ kit allows the easy distinction between non-apoptotic red fluorescent cells and apoptotic green fluorescent cells. |
|
| M1563 |
Astra Blue 6GLL |
82864-57-1 |
25 g |
113.40 |
90.72 |
| Stain for oligo- and polysaccharides such as cellulose in plant tissues. Has also been used for analysis of human mast cells from tissues or blood samples. |
|
| M1274 |
Avidin fluorescein isothiocyanate (FITC) conjugate |
N/A |
0 |
0.00 |
0.00 |
| This green fluorescent protein excites at 494 nm with an emission maximum at 518 nm. Since the excitation and emission maxima are well separated from those of other fluorophores such as Texas RedTM and coumarin, FITC Avidin can be employed with other conjugates to simultaneously localize two or three antigens in the same tissue section. |
|
| M1124 |
Avidin sulforhodamine 101 conjugate (Texas Red™ equivalent) |
N/A |
5 mg |
142.87 |
114.30 |
| This red fluorescent protein excites at 595 nm with an emission maximum at 615 nm. Since the excitation and emission maxima are well separated from those of fluorescein, this highly fluorescent conjugate can be employed with other fluorescein or coumarin conjugates to simultaneously localize two or three antigens in the same tissue section. |
|
| M0384 |
8-Azidoadenosine 5'-triphosphate, TEA salt |
N/A |
10 mg |
160.43 |
128.34 |
| Useful in photoaffinity labelling of ATP binding proteins. Covalent labeling after exposure to UV light (e.g. UVS-11 or UVG-11 lamp). Not radiolabeled. |
|
| M1251 |
3-(2-Benzoxazolyl)umbelliferone |
64887-40-7 |
100 mg |
61.33 |
49.07 |
| Blue-green fluorescent coumarin derivative useful for staining cells and tissues. Also used as a laser dye and for doping in polymer applications. |
|
| M1253 |
3-(2-Benzoxazolyl)umbelliferyl acetate |
97004-78-9 |
25 mg |
87.90 |
70.32 |
| Fluorogenic esterase substrate with blue-green emission (468 nm) upon enzyme activity. |
|
| M0182 |
7-N-Benzoyl-cephalosporanic acid, L-glutamate ester |
N/A |
5 mg |
106.95 |
85.56 |
| Conjugate of Cephalosporin C and glutamate useful for release of the essential amino-acid L-glutamate, upon action of ampicillinase (beta-lactamase activity) in amp-positive cell lines from vectors incorporating the amp (selection) marker gene. |
|
| M1257 |
Berberine sulfate |
633-66-9 |
25 mg |
65.20 |
52.16 |
| Useful as a fluorescent stain for heparin in several cell lines. |
|
| M0128 |
Biocytin hydrazide hydrochloride |
102743-85-1 |
10 mg |
53.48 |
42.78 |
| Water-soluble, long-chained, biotin-containing hydrazide, used for the selective detection of glycoconjugates. |
|
| M1356 |
MarkerGene™ Biotin Detection and Quantitation Kit |
|
1 kit |
120.75 |
96.60 |
| Allows the measurement of the molar ratio of biotin to protein in biotin-protein conjugates, biotin-DNA molar ratios in biotinylated DNA samples, or quantitation of biotin concentrations in solution. The kit can be used to determine biotin concentrations down to 1 uM concentrations. The assay can be performed in a microtiterplate format. |
|
| M1624 |
OliGlo™ Biotin FISH Kit - trial size |
|
|
245.00 |
|
| Fluorescence in situ hybridization (FISH) is a cytogenetic technique used to detect and localize the presence or absence of specific DNA sequences on chromosomes. FISH uses fluorescent probes that bind to only those parts of the chromosome with which they show a high degree of sequence similarity. Fluorescence microscopy can be used to find out where the fluorescent probe is bound to the chromosome. FISH is often employed for discovery of specific features in DNA and is useful in genetic analysis, medicine, or species identification. FISH can also be used to detect and localize specific mRNAs within tissue samples. In this context, it can help define the spatial-temporal patterns of gene expression within cells and tissues.
The probes used in these analyses are typically short complimentary sequences to the target DNA which can be fluorescently labeled using this kit. In a typical protocol, metaphase chromosome spreads are prepared on glass slides, RNase treated, dehydrated and denatured. The slides are then hybridized with the fluorescently labeled DNA probes for 16-24 hours. Post hybridization, the slides are washed, counterstained with a 4’,6-diamidino-2-phenylindole (DAPI) / antifade mixture, and analyzed using fluorescence microscopy.
Our OliGlo™ kits directly label nucleic acid through reaction with the phosphate groups (terminal and backbone) of the probe nucleotide providing cleaner hybridization analysis.
|
|
| M1625 |
OliGlo™ Biotin FISH Kit - standard size |
|
|
720.00 |
|
| Fluorescence in situ hybridization (FISH) is a cytogenetic technique used to detect and localize the presence or absence of specific DNA sequences on chromosomes. FISH uses fluorescent probes that bind to only those parts of the chromosome with which they show a high degree of sequence similarity. Fluorescence microscopy can be used to find out where the fluorescent probe is bound to the chromosome. FISH is often employed for discovery of specific features in DNA and is useful in genetic analysis, medicine, or species identification. FISH can also be used to detect and localize specific mRNAs within tissue samples. In this context, it can help define the spatial-temporal patterns of gene expression within cells and tissues.
The probes used in these analyses are typically short complimentary sequences to the target DNA which can be fluorescently labeled using this kit. In a typical protocol, metaphase chromosome spreads are prepared on glass slides, RNase treated, dehydrated and denatured. The slides are then hybridized with the fluorescently labeled DNA probes for 16-24 hours. Post hybridization, the slides are washed, counterstained with a 4’,6-diamidino-2-phenylindole (DAPI) / antifade mixture, and analyzed using fluorescence microscopy.
Our OliGlo™ kits directly label nucleic acid through reaction with the phosphate groups (terminal and backbone) of the probe nucleotide providing cleaner hybridization analysis.
|
|
| M0785 |
Biotin succinimidyl ester |
35013-72-0 |
100 mg |
60.80 |
48.64 |
| Activated biotin derivative which can be readily coupled to primary or secondary amines of analyte compounds or proteins under mild conditions. |
|
| M1604 |
OliGlo™ Biotin Universal Nucleic Acid Labeling Kit - trial size |
|
|
175.00 |
|
| A number of agents have been described for labeling nucleic acids to facilitate detection of target DNA or RNA sequences. Suitable labels may provide signals detectable by fluorescence, radioactivity, colorimetry, X-ray diffraction or absorption, magnetism or enzymatic activity. It is essential that the labeling method not perturb base-pairing hybridization critical for preserving assay specificity. Nevertheless, several common methods including labeling by enzymatic incorporation can often lead to interference with the subsequent hybridization detection step, because current fluorescent labels are attached to the base (purine, pyrimidine) portion of the nucleotides where base-pairing and hybridization occurs.
To remedy this, methods of direct labeling have been used with varying degrees of success. Direct labeling through phosphodiester and phosphotriester linkages on the DNA or RNA backbone provides the additional advantage of reducing the perturbation of base-pairing hybridization. Our OliGlo™ kits utilize a direct labeling methodology through reaction with the phosphate groups (terminal and backbone) on oligonucleotides, DNA or RNA. The active labeling reagents are prepared in situ from stable precursor molecules derived from a variety of highly fluorescent dyes and other detection labels, allowing the highly reactive labels to function at optimum efficiency for each sample. The OliGlo™ kits allow molecular biologists and clinical researchers to label or monitor genomic DNA or RNA samples, nucleotides or oligonucleotides for easy detection and quantification.
|
|
| M1605 |
OliGlo™ Biotin Universal Nucleic Acid Labeling Kit- standard size |
|
|
480.00 |
|
| A number of agents have been described for labeling nucleic acids to facilitate detection of target DNA or RNA sequences. Suitable labels may provide signals detectable by fluorescence, radioactivity, colorimetry, X-ray diffraction or absorption, magnetism or enzymatic activity. It is essential that the labeling method not perturb base-pairing hybridization critical for preserving assay specificity. Nevertheless, several common methods including labeling by enzymatic incorporation can often lead to interference with the subsequent hybridization detection step, because current fluorescent labels are attached to the base (purine, pyrimidine) portion of the nucleotides where base-pairing and hybridization occurs.
To remedy this, methods of direct labeling have been used with varying degrees of success. Direct labeling through phosphodiester and phosphotriester linkages on the DNA or RNA backbone provides the additional advantage of reducing the perturbation of base-pairing hybridization. Our OliGlo™ kits utilize a direct labeling methodology through reaction with the phosphate groups (terminal and backbone) on oligonucleotides, DNA or RNA. The active labeling reagents are prepared in situ from stable precursor molecules derived from a variety of highly fluorescent dyes and other detection labels, allowing the highly reactive labels to function at optimum efficiency for each sample. The OliGlo™ kits allow molecular biologists and clinical researchers to label or monitor genomic DNA or RNA samples, nucleotides or oligonucleotides for easy detection and quantification.
|
|
| M0788 |
Biotin-dextran (70,000 MW) |
N/A |
10 mg |
66.87 |
53.50 |
| Dextran conjugate containing covalently attached biotin molecules (Vitamin H) making it useful in streptavidin/avidin assay systems. It is membrane impermeant but can be loaded into cells using either microinjection or by hypoosmotic shock conditions. |
|
| M1138 |
MarkerGene™ Biotin-X Antibody/Protein Labeling Kit |
|
1 kit |
233.10 |
186.48 |
| For efficient biotinylation of antibodies and other proteins. The long spacer between the biotin and the reactive group in biotin-X succinimidyl ester enhances the ability of the conjugated biotin to interact with avidin and streptavidin. |
|
| M0783 |
Biotin-X, SE |
72040-63-2 |
50 mg |
54.71 |
43.76 |
| Reactive biotin derivative containing an aminohexanoyl spacer group (termed "X") between biotin and the binding site for proteins, nucleic acids, or other biological molecules. This spacer helps to reduce potential quenching effects of fluorophores attached to the analyte molecule, since biotin binds to "deep" binding sites in avidin or streptavidin. |
|
| M1530 |
11-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-yl)undecanoic acid NHS ester ( BODIPY® FL C11, SE) |
|
5 mg |
236.04 |
188.83 |
| Useful fluorescent amine-reactive probe has an added spacer to improve accessibility of the reactive group by nucleophiles in preparation of bioconjugates. Substitute for fluorescein providing pH-insensitive fluorescence and narrow spectral bandwidth.
|
|
| M0632 |
Bromoxynil glucuronic acid methyl ester |
for bromoxynil 1689-84-5 |
2 mg |
77.50 |
62.00 |
| Bromoxynil derivative which can be used to selectively ablate GUS-positive cells and tissues in plant systems. The methyl ester helps with cell permeation in vivo. |
|
| M0630 |
Bromoxynil glucuronic acid triacetate methyl ester |
[1689-84-5] for bromoxynil aglycone |
2 mg |
77.50 |
62.00 |
| Bromoxynil derivative which can be used for selective release in GUS-positive plant systems, in a tissue specific manner. Additional ester groups help with cell permeation of this herbicide derivative. |
|
| M1080 |
C12-NBD, free acid (12-(7-Nitrobenzofurazan-4-ylamino)dodecanoic acid) |
96801-39-7 |
100 mg |
121.54 |
97.23 |
| Useful fluorescent probe for fatty acid and carrier protein binding sites. |
|
| M1545 |
Calcein |
1461-15-0 |
25 mg |
43.58 |
34.86 |
| Fluorescent calcium indicator. |
|
| M1547 |
Calcein-AM |
148504-34-1 |
100 μl (4mM soln) |
116.03 |
92.82 |
| For determining cell viability. In live cells the non-fluorescent calcein AM is hydrolyzed by intracellular esterases into the green fluorescent anion calcein. |
|
| M0272 |
MarkerGene™ Carbohydrate Analysis Detection Kit |
|
1 kit |
209.48 |
167.58 |
| Allows the user to quickly estimate and/or compare the composition of carbohydrates obtained from glycoproteins. |
|
| M1400 |
5(6)-Carboxy-X-rhodamine |
198978-94-8 |
25 mg |
88.30 |
70.64 |
| Stable, long wavelength, water soluble fluorophore for use in DNA-labeling, cell staining and protein labeling applications. |
|
| M1469 |
5-Carboxy-X-Rhodamine |
|
5 mg |
92.40 |
73.92 |
| This dye is commonly used in dye-terminator sequencing methods by attachment to dideoxynucleotide triphosphates (ddNTP's). It can also be used in other DNA or RNA labeling methods.
|
|
| M1470 |
6-Carboxy-X-Rhodamine |
194785-18-7 |
5 mg |
92.40 |
73.92 |
| This dye is commonly used in dye-terminator sequencing methods by attachment to dideoxynucleotide triphosphates (ddNTP's). It can also be used in other DNA or RNA labeling methods.
|
|
| M1471 |
5-Carboxy-X-Rhodamine, NHS Ester |
344402-35-3 |
5 mg |
273.53 |
218.82 |
| This dye is commonly used in dye-terminator sequencing methods by attachment to dideoxynucleotide triphosphates (ddNTP's). It can also be used in other DNA or RNA labeling methods.
|
|
| M1472 |
6-Carboxy-X-Rhodamine, NHS Ester |
216699-36-4 |
5 mg |
200.03 |
160.02 |
| This dye is commonly used in dye-terminator sequencing methods by attachment to dideoxynucleotide triphosphates (ddNTP's). It can also be used in other DNA or RNA labeling methods.
|
|
| M1239 |
5(6)-Carboxy-2',7'-dichlorofluorescein |
111843-78-8 |
50 mg |
34.02 |
27.22 |
| Fluorescein analog with high fluorescence in the low pH range (pH 4-5). Useful for intracellular organelle staining of acidic vesicles. |
|
| M1300 |
5(6)-Carboxyeosin |
132201-84-4 |
100 mg |
64.05 |
51.24 |
| Brominated analog of carboxyfluorescein that is a useful photosensitizer and singlet oxygen generator. |
|
| M0114 |
2,7-bis(2-Carboxyethyl)-5(6)-carboxyfluorescein (BCECF) |
85138-49-4 |
10 mg |
91.22 |
72.98 |
| Widely used fluorescent pH indicator for cellular and in vitro applications. This AM-ester derivative is membrane permeable and useful for staining live cells. |
|
| M0122 |
5(6)-Carboxyfluorescein |
72088-94-9 |
100 mg |
24.36 |
19.49 |
| Extremely useful fluorescent reagent for monitoring intracellular pH and for general labeling of cells and tissues due to its high extinction coefficient, water solubility and pH sensitivity. |
|
| M0011 |
5(6)-Carboxyfluorescein diacetate (CFDA) |
124387-19-5 |
100 mg |
113.93 |
91.14 |
| Popular, ultrasensitive, fluorescent substrate for measuring esterase activity in live cells. It is often used in cell viability assays and "Live-Dead" assay systems |
|
| M0013 |
5(6)-Carboxyfluorescein diacetate, NHS ester |
150347-59-4 |
25 mg |
121.54 |
97.23 |
| Labeling reagent (amine-reactive) with green emission spectral properties (Abs 488 nm / EM 517 nm) after intracellular removal of acetate esters.
|
|
| M0984 |
5(6)-Carboxyfluorescein, NHS ester |
117548-22-8 |
25 mg |
30.34 |
24.28 |
| Useful fluorescent amine-reactive probe for synthesis of bioconjugates, yielding hydrolysis resistant derivatives which show excellent performance and stability at physiological pH values. |
|
| M1248 |
5(6)-Carboxynaphthofluorescein |
128724-35-6 |
25 mg |
48.62 |
38.89 |
| pH-dependent fluorophore with red fluorescence (EX598/EM668 nm at pH 9) and a high pKa (7.6). The fluorescence shifts to shorter wavelengths (EX512/EM567 nm) at neutral or acidic pH. |
|
| M1259 |
5(6)-Carboxynaphthofluorescein, N-succinimidyl ester |
150347-58-3 |
10 mg |
36.15 |
28.92 |
| Reactive long wavelength fluorophore for use in labeling proteins and other compounds. Reactive with terminal and internal (Lys) groups, at pH 8-9. The fluorescence properties are pH dependent.
|
|
| M0641 |
5(6)-Carboxyrhodamine 110, HCl salt |
116763-35-0 |
50 mg |
147.03 |
117.62 |
| Stable, long wavelength, water soluble fluorophore for use in DNA-labeling, cell staining and protein labeling applications. |
|
| M0962 |
5(6)-Carboxytetramethylrhodamine (TAMRA) |
98181-63-6 |
100 mg |
140.70 |
112.56 |
| Common DNA labeling reagent, also used for protein labeling. |
|
| M0966 |
5(6)-Carboxytetramethylrhodamine N-succinimidyl ester |
246256-50-8 |
|
|
|
|
|
| M0625 |
3-Carboxyumbelliferone, BSA conjugate |
N/A |
2 mg |
73.57 |
58.86 |
| Useful for endocytosis experiments or microinjection analyses. |
|
| M0257 |
Carboxyumbelliferyl β-D-galactopyranoside (CUG) |
64664-99-9 |
5 mg |
113.93 |
91.14 |
| Blue fluorogenic beta-galactosidase substrate that is well retained intracellularly. The fluorescence spectrum for the released 3-carboxyumbelliferone is pH-dependent. |
|
| M0611 |
3-Carboxyumbelliferyl β-D-galactoside, BSA conjugate |
N/A |
2 mg |
86.86 |
69.49 |
| Useful for detection of extracellular galactosidase activity, from enzyme-linked antibody systems or for endocytosis experiments, measurement of lacY permease activity, or microinjection analyses. |
|
| M0256 |
Carboxyumbelliferyl β-D-glucuronide (CUGlcU) |
N/A |
10 mg |
80.27 |
64.22 |
| Water soluble fluorogenic GUS substrate similar to MUG. |
|
| M0818 |
Green FAM Caspase 1 Assay Kit 100tests |
N/A |
1 kit |
487.20 |
487.20 |
| FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. |
|
| M0817 |
Green FAM Caspase 1 Assay Kit 25tests |
N/A |
1 kit |
177.45 |
177.45 |
| FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. |
|
| M0830 |
Green FAM Caspase 10 Assay Kit 100tests |
N/A |
1 kit |
487.20 |
487.20 |
| FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. |
|
| M0829 |
Green FAM Caspase 10 Assay Kit 25tests |
N/A |
1 kit |
177.45 |
177.45 |
| FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. |
|
| M0832 |
Green FAM Caspase 13 Assay Kit 100tests |
N/A |
1 kit |
487.20 |
487.20 |
| FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. |
|
| M0831 |
Green FAM Caspase 13 Assay Kit 25tests |
N/A |
1 kit |
177.45 |
177.45 |
| FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. |
|
| M0820 |
Green FAM Caspase 2 Assay Kit 100tests |
N/A |
1 kit |
487.20 |
487.20 |
| FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. |
|
| M0819 |
Green FAM Caspase 2 Assay Kit 25tests |
N/A |
1 kit |
177.45 |
177.45 |
| FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. |
|
| M0822 |
Green FAM Caspases 3 and 7 Assay Kit 100tests |
N/A |
1 kit |
487.20 |
487.20 |
| FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. |
|
| M0836 |
Red Sulforhodamine Caspases 3 and 7 Assay Kit 100tests |
|
|
|
|
|
|
| M0838 |
Magic Red™-(DEVD)2 Caspases 3 and 7 Assay Kit 100tests |
N/A |
1 kit |
413.70 |
413.70 |
| Magic Red™ products are substrate-based assays designed to detect protease activity within whole living cells, using a fluorescence microscope. |
|
| M0821 |
Green FAM Caspases 3 and 7 Assay Kit 25tests |
N/A |
1 kit |
177.45 |
177.45 |
| FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. |
|
| M0835 |
Red Sulforhodamine Caspases 3 and 7 Assay Kit 25tests |
|
|
|
|
|
|
| M0837 |
Magic Red™-(DEVD)2 Caspases 3 and 7 Assay Kit 25tests |
N/A |
1 kit |
166.95 |
166.95 |
| Magic Red™ products are substrate-based assays designed to detect protease activity within whole living cells, using a fluorescence microscope. |
|
| M0824 |
Green FAM Caspase 6 Assay Kit 100tests |
N/A |
1 kit |
487.20 |
487.20 |
| FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. |
|
| M0823 |
Green FAM Caspase 6 Assay Kit 25tests |
N/A |
1 kit |
177.45 |
177.45 |
| FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. |
|
| M0826 |
Green FAM Caspase 8 Assay Kit 100tests |
N/A |
1 kit |
487.20 |
487.20 |
| FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. |
|
| M0825 |
Green FAM Caspase 8 Assay Kit 25tests |
N/A |
1 kit |
177.45 |
177.45 |
| FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. |
|
| M0828 |
Green FAM Caspase 9 Assay Kit 100tests |
N/A |
1 kit |
487.20 |
487.20 |
| FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. |
|
| M0827 |
Green FAM Caspase 9 Assay Kit 25tests |
N/A |
1 kit |
177.45 |
177.45 |
| FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. |
|
| M0816 |
Green FAM Poly Caspases Assay Kit 100tests |
N/A |
1 kit |
487.20 |
487.20 |
| FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. |
|
| M0815 |
Green FAM Poly Caspases Assay Kit 25 tests |
N/A |
1 kit |
177.45 |
177.45 |
| FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. |
|
| M0834 |
Red Sulforhodamine Poly Caspases Detection Kit 100tests |
N/A |
1 kit |
518.70 |
518.70 |
| SR-FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases. |
|
| M0833 |
Red Sulforhodamine Poly Caspases Detection Kit 25tests |
N/A |
1 kit |
193.20 |
193.20 |
| SR-FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases. |
|
| M0840 |
Magic Red™-(RR)2 Cathepsin B Assay Kit 100tests |
N/A |
1 kit |
413.70 |
413.70 |
| These Magic Red™ substrate-based assays are designed to detect Cathepsin protease activity within whole living cells, using fluorescence microscopy. |
|
| M0839 |
Magic Red™-(RR)2Cathepsin B Assay Kit 25tests |
N/A |
1 kit |
166.95 |
166.95 |
| These Magic Red™ substrate-based assays are designed to detect Cathepsin protease activity within whole living cells, using fluorescence microscopy. |
|
| M0841 |
Magic Red™-(LR)2 Cathepsin K Assay Kit 25tests |
N/A |
1 kit |
166.95 |
166.95 |
| These Magic Red™ substrate-based assays are designed to detect Cathepsin protease activity within whole living cells, using fluorescence microscopy. |
|
| M0844 |
Magic Red™-(FR)2 Cathepsin L Assay Kit 100tests |
N/A |
1 kit |
413.70 |
413.70 |
| These Magic Red™ substrate-based assays are designed to detect Cathepsin protease activity within whole living cells, using fluorescence microscopy. |
|
| M0843 |
Magic Red™-(FR)2 Cathepsin L Assay Kit 25tests |
N/A |
1 kit |
166.95 |
166.95 |
| These Magic Red™ substrate-based assays are designed to detect Cathepsin protease activity within whole living cells, using fluorescence microscopy. |
|
| M0353 |
N-CBZ-β-glycinyl-6-aminoluciferin |
1233518-28-9 |
2 mg |
211.25 |
169.00 |
| Protected glycine derivative produces the chemiluminescent compound 6-Amino-D-Luciferin Product M0352 upon peptidase enzymatic reaction. |
|
| M1405 |
MarkerGene™ Cellular Senescence Microtiterplate Assay Kit |
|
1 Kit |
200.03 |
160.02 |
| Uses the high-sensitivity substrate fluorescein di-β-D-galactopyranoside (FDG) to quantify senescent cells. |
|
| M0855 |
MarkerGene™ Chemiluminescent lacZ β-Galactosidase Detection Kit |
|
1 kit |
240.24 |
192.19 |
| Allows for quantitative measure of β-galactosidase enzyme activity in mammalian, yeast, and bacterial cells through use of a chemiluminescent substrate. |
|
| M0165 |
Chloramphenicol β-D-galactoside |
191476-32-1 |
2 mg |
106.95 |
85.56 |
| Upon enzymatic or chemical hydrolysis of the galactoside group, chloramphenicol, an antibiotic (bacteriostatic), is produced. |
|
| M0637 |
2-Chloro-4-nitrophenyl-β-D-cellobioside |
135743-28-1 |
25 mg |
66.87 |
53.50 |
| Chromogenic substrate for cellulase activity. |
|
| M0553 |
2-Chloro-4-nitrophenyl-β-D-lactoside |
120583-41-7 |
25 mg |
106.95 |
85.56 |
| Chromogenic substrate for lactosidase activity. The released product, 2-chloro-4-nitrophenol (CNP) is usually monitored spectrophotometrically at 405 nm. The rate of formation of 2-chloro-4-nitrophenol is proportional
to the lactosidase activity in the sample. The pKa of CNP is approximately 5.5. |
|
| M0739 |
Coelenterazine (native form) |
55779-48-1 |
50 μl* |
44.21 |
35.36 |
| The native form of the luminescent enzyme substrate for apoaequorin, gaussia and renilla luciferases. This substrate is also a component of
our convenient assay kit, MarkerGene™ Gaussia Luciferase Cellular Assay Kit,
Product M1193, for quickly measuring the luciferase activity in mammalian cell culture. |
|
| M1622 |
OliGlo™Cy3 FISH Kit - trial size |
|
|
245.00 |
|
| Fluorescence in situ hybridization (FISH) is a cytogenetic technique used to detect and localize the presence or absence of specific DNA sequences on chromosomes. FISH uses fluorescent probes that bind to only those parts of the chromosome with which they show a high degree of sequence similarity. Fluorescence microscopy can be used to find out where the fluorescent probe is bound to the chromosome. FISH is often employed for discovery of specific features in DNA and is useful in genetic analysis, medicine, or species identification. FISH can also be used to detect and localize specific mRNAs within tissue samples. In this context, it can help define the spatial-temporal patterns of gene expression within cells and tissues.
The probes used in these analyses are typically short complimentary sequences to the target DNA which can be fluorescently labeled using this kit. In a typical protocol, metaphase chromosome spreads are prepared on glass slides, RNase treated, dehydrated and denatured. The slides are then hybridized with the fluorescently labeled DNA probes for 16-24 hours. Post hybridization, the slides are washed, counterstained with a 4’,6-diamidino-2-phenylindole (DAPI) / antifade mixture, and analyzed using fluorescence microscopy.
Our OliGlo™ kits directly label nucleic acid through reaction with the phosphate groups (terminal and backbone) of the probe nucleotide providing cleaner hybridization analysis.
|
|
| M1623 |
OliGlo™Cy3 FISH Kit - standard size |
|
|
720.00 |
|
| Fluorescence in situ hybridization (FISH) is a cytogenetic technique used to detect and localize the presence or absence of specific DNA sequences on chromosomes. FISH uses fluorescent probes that bind to only those parts of the chromosome with which they show a high degree of sequence similarity. Fluorescence microscopy can be used to find out where the fluorescent probe is bound to the chromosome. FISH is often employed for discovery of specific features in DNA and is useful in genetic analysis, medicine, or species identification. FISH can also be used to detect and localize specific mRNAs within tissue samples. In this context, it can help define the spatial-temporal patterns of gene expression within cells and tissues.
The probes used in these analyses are typically short complimentary sequences to the target DNA which can be fluorescently labeled using this kit. In a typical protocol, metaphase chromosome spreads are prepared on glass slides, RNase treated, dehydrated and denatured. The slides are then hybridized with the fluorescently labeled DNA probes for 16-24 hours. Post hybridization, the slides are washed, counterstained with a 4’,6-diamidino-2-phenylindole (DAPI) / antifade mixture, and analyzed using fluorescence microscopy.
Our OliGlo™ kits directly label nucleic acid through reaction with the phosphate groups (terminal and backbone) of the probe nucleotide providing cleaner hybridization analysis.
|
|
| M1602 |
OliGlo™ Cy3 Universal Nucleic Acid Labeling Kit - trial size |
|
|
175.00 |
|
| Our OliGlo™ kits utilize a direct labeling methodology through reaction with the phosphate groups (terminal and backbone) on oligonucleotides, DNA or RNA. The active labeling reagents are prepared in situ from stable precursor molecules derived from a variety of highly fluorescent dyes and other detection labels, allowing the highly reactive labels to function at optimum efficiency for each sample. The OliGlo™ kits allow molecular biologists and clinical researchers to label or monitor genomic DNA or RNA samples, nucleotides or oligonucleotides for easy detection and quantification. |
|
| M1603 |
OliGlo™ Cy3 Universal Nucleic Acid Labeling Kit - standard size |
|
|
480.00 |
|
| A number of agents have been described for labeling nucleic acids to facilitate detection of target DNA or RNA sequences. Suitable labels may provide signals detectable by fluorescence, radioactivity, colorimetry, X-ray diffraction or absorption, magnetism or enzymatic activity. It is essential that the labeling method not perturb base-pairing hybridization critical for preserving assay specificity. Nevertheless, several common methods including labeling by enzymatic incorporation can often lead to interference with the subsequent hybridization detection step, because current fluorescent labels are attached to the base (purine, pyrimidine) portion of the nucleotides where base-pairing and hybridization occurs.
To remedy this, methods of direct labeling have been used with varying degrees of success. Direct labeling through phosphodiester and phosphotriester linkages on the DNA or RNA backbone provides the additional advantage of reducing the perturbation of base-pairing hybridization. Our OliGlo™ kits utilize a direct labeling methodology through reaction with the phosphate groups (terminal and backbone) on oligonucleotides, DNA or RNA. The active labeling reagents are prepared in situ from stable precursor molecules derived from a variety of highly fluorescent dyes and other detection labels, allowing the highly reactive labels to function at optimum efficiency for each sample. The OliGlo™ kits allow molecular biologists and clinical researchers to label or monitor genomic DNA or RNA samples, nucleotides or oligonucleotides for easy detection and quantification.
|
|
| M1383 |
Dansyl chloride |
605-65-2 |
1 g |
41.48 |
33.18 |
| Widely used in protein sequencing and amino acid analysis. |
|
| M1385 |
Dansyl hydrazine |
33008-06-9 |
100 mg |
53.55 |
42.84 |
| For use in HPLC labeling. |
|
| M1127 |
Dansyl-β-lactoglobulin-A |
N/A |
1 mg |
40.77 |
32.61 |
| IEF-Marker for HPCE and for IEF-gels. Fluorescent IEF Marker pI 5.1 with EX 330nm and EM 415 nm in 0.1 M citrate buffer pH 5.1. |
|
| M1249 |
Dansyl-11-aminoundecanoic acid |
73025-02-2 |
25 mg |
35.81 |
28.65 |
| Ambiphilic probe with a polar, environmentally-sensitive dansyl fluorophore attached to a long chain fatty acid. The polar dansyl headgroup partitions to the surface of lipid bilayer membranes while the fatty acid portion embeds into lipid bilayers or can attach to fatty acid carrier protein lipidic environments. |
|
| M0329 |
3-Dansylaminophenylboronic acid |
75806-94-9 |
50 mg |
37.94 |
30.34 |
| Fluorescent labeling agent for cis-diols (sugars) at high pH values on cell surfaces and in vitro for carbohydrate determination in glycoproteins and for HPLC analysis of sugars. |
|
| M0189 |
3,5-Di-O-(p-toluyl)-2-deoxy-D-ribofuranosyl chloride |
4330-21-6 |
1 g |
379.84 |
303.87 |
| Intermediate for deoxynucleotide synthesis. This reactive anomeric chloride derivative reacts with nucleophilic bases to provide β-D-ribofuranosyl glycosides. |
|
| M0149 |
Dexamethasone-21-O-β-D-galactopyranoside |
319426-57-8 |
1 mg |
106.95 |
85.56 |
| Cleavage product: Upon enzymatic or chemical hydrolysis of the galactoside group, dexamethasone is produced. |
|
| M0169 |
alpha-DGal(1-4)DGal - CETE - BSA Conjugate |
N/A |
1 mg |
240.70 |
192.56 |
| Represents a portion of the P1 blood group antigen that is responsible for E. coli adhesion to surface membranes of the intestinal tract. Bound to Bovine Serum Albumin (BSA), it is a useful antigen for immunological studies or as an inhibitor of adhesion of endothelial cell binding to uropathogenic E. coli strains. |
|
| M0108 |
alpha-DGal(1-4)DGal-O-Me |
N/A |
5 mg |
33.38 |
26.70 |
| Represents a portion of the P1 blood group antigen that is responsible for E. coli adhesion to surface membranes of the intestinal tract. It is a useful antigen for immunological studies or as an inhibitor of adhesion of endothelial cell binding to uropathogenic E. coli strains. |
|
| M0530 |
N-(di-Methyl amino naphthyl)-N-(para carboxy phenyl) hydrazide |
N/A |
2 mg |
160.43 |
128.34 |
| Long wavelength azo dye is a useful reagent (similar to DABCYL-succinimidyl ester) for labeling proteins or peptides through their amino groups. |
|
| M0532 |
N-(di-Methyl amino naphthyl)-N-(para carboxy phenyl) hydrazide, NHS ester |
N/A |
1 mg |
160.43 |
128.34 |
| Longer wavelength azo dye quencher is a useful reagent (similar to DABCYL-succinimidyl ester) for labeling proteins or peptides through their amino groups. |
|
| M0220 |
1,2-Di-O-octanoyl galactopyranosyl rac-glycerol |
319426-70-5 |
10 mg |
80.27 |
64.22 |
| Synthetic diglyceride analog is an activator of protein kinase C, that is released upon action of β-galactosidase in lacZ transfected cells and tissues. |
|
| M1278 |
2',7'-Dichlorofluorescein |
76-54-0 |
100 mg |
52.29 |
41.83 |
| Green fluorophore standard used in enzyme assay systems. |
|
| M1194 |
2', 7'-Dichlorofluorescein di-β-D-galactopyranoside (DCFDG) |
N/A |
25 mg |
83.74 |
66.99 |
| Highly sensitive fluorescent substrate for measuring galactosidase and galactocerebrosidase activity inside of live cells and lysosomes. |
|
| M0807 |
2', 7'-Dichlorofluorescin diacetate |
4091-99-0 |
25 mg |
48.62 |
38.89 |
| Reduced derivative of 2',7'-dichlorofluorescein is a cell permeable reagent for use in detection of peroxidase activity (oxidative metabolism, intracellular regulation of reactive oxygen species or oxidative burst) inside living cells. |
|
| M1456 |
9-(2,2-Dicyanovinyl)julolidine |
58293-56-4 |
5 mg |
34.86 |
27.89 |
| Fluorescent probe useful for binding to proteins. |
|
| M0853 |
7-(Diethylamino)coumarin-3-carbonyl azide |
157673-16-0 |
25 mg |
115.00 |
96.00 |
| Diethylaminocoumarin-3-carbonyl azide is an amine reactive probe useful for labeling proteins or other amine containing compounds. It also has found use in HPLC labeling of lipids and alkylcycloketones. |
|
| M1053 |
7-(Diethylamino)coumarin-3-carboxylic acid, N-succinimidyl ester |
139346-57-9 |
25 mg |
80.27 |
64.22 |
| Amine reactive fluorescent probe useful for labeling amino acids prior to resolution by capillary zone electrophoresis and for multicolor DNA and protein labeling experiments. |
|
| M0851 |
7-(Diethylamino)coumarin-3-carboxylic acid |
50995-74-9 |
100 mg |
66.81 |
53.45 |
| Blue-green fluorescent label for use in peptide and protein modification. |
|
| M1241 |
Dihydroethidium |
104821-25-2 |
10 mg |
52.47 |
41.98 |
| Shows a blue fluorescence (absorption/emission: 355/420nm) in cell cytoplasm until oxidization to form ethidium which becomes red fluorescent (absorption/emission: 518/605 nm) upon DNA intercalation. |
|
| M0545 |
Dihydrorhodamine 123 (DHR123) |
109244-58-8 |
2 mg |
45.55 |
36.44 |
| One of the most widely used Reactive Oxygen Species (ROS) probes for intracellular measurement and analysis. |
|
| M1051 |
4-[4-(Dimethylamino)phenylazo]benzoic acid, N-succinimidyl ester (DABCYL-SE) |
146998-31-4 |
25 mg |
33.38 |
26.70 |
| Long wavelength azo quencher is a useful reagent (similar to MANCYL-SE) for labeling proteins or peptides through their amino-groups. The succinimidyl ester is reactive with terminal amines or lysines of peptides and other nucleophiles for labeling studies of proteins. |
|
| M1197 |
3,3-Dioctadecyloxacarbocyanine perchlorate (DiO; DiOC18(3)) |
34215-57-1 |
25 mg |
50.13 |
40.10 |
| Highly fluorescent membrane probe for labeling live and fixed cells in culture. |
|
| M1269 |
1,1'-Dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine perchlorate |
127274-91-3 |
10 mg |
53.45 |
42.76 |
| Red fluorescent membrane probe for long term staining of live cells. Also used in fiber optic sensors for detecting K+ using fluorescence energy transfer.
|
|
| M1577 |
1,1'-Dioctadecyl-3,3,3',3'-tetramethylindotricarbocyanine iodide |
100068-60-8 |
25 mg |
77.70 |
62.16 |
| A lipophilic near-IR (NIR) fluorescent membrane probe, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindotricarbocyanine iodide (DiR)has been used for labeling cells for long term analysis. This tricarbocyanine fluorophore is excited at 750 nm and has an emission peak at 782 nm. It also has virtually no fluorescence in water, while having high fluorescence when incorporated into cell membranes. |
|
| M0258 |
1,2-Dioleoyl-3-(pyren-1-yl) decanoyl-rac-glycerol |
102807-51-2 |
1 mg |
120.35 |
96.29 |
| Useful for measuring cholesteryl ester transfer and protein-mediated triacylglycerol transport between plasma lipoproteins. Has been found to be an excellent substrate for lipoprotein lipase and hepatic triacylglycerol lipase. See our kit M0612 for more information and protocols for use. |
|
| M1331 |
3,3'-Dipropylthiacarbocyanine iodide |
53336-12-2 |
250 mg |
41.90 |
33.52 |
| Membrane potential sensitive dye for monitoring changes in the cellular and mitochondrial membrane potential in vivo. |
|
| M1337 |
EDANS-Fucoside |
N/A |
1 mg |
41.48 |
33.18 |
| Standard for MarkerGene™ Carbohydrate Analysis Detection Kit M0272. |
|
| M1338 |
EDANS-Galactoside |
N/A |
1 mg |
41.48 |
33.18 |
| Standard for MarkerGene™ Carbohydrate Analysis Detection Kit M0272. |
|
| M1478 |
EDANS-GalNAc |
N/A |
1 mg |
41.48 |
33.18 |
| Standard for MarkerGene™
Carbohydrate Analysis Detection Kit M0272. |
|
| M1339 |
EDANS-GlcNAc |
N/A |
1 mg |
41.48 |
33.18 |
| Standard for MarkerGene™ Carbohydrate Analysis Detection Kit M0272. |
|
| M1336 |
EDANS-Glucoside |
N/A |
1 mg |
41.48 |
33.18 |
| Standard for MarkerGene™ Carbohydrate Analysis Detection Kit M0272. |
|
| M1335 |
EDANS-Mannoside |
N/A |
1 mg |
41.48 |
33.18 |
| Standard for MarkerGene™ Carbohydrate Analysis Detection Kit M0272. |
|
| M1480 |
EDANS-Neuraminic Acid |
N/A |
1 mg |
41.48 |
33.18 |
| Standard for MarkerGene™
Carbohydrate Analysis Detection Kit M0272. |
|
| M1479 |
EDANS-Xyloside |
N/A |
1 mg |
41.48 |
33.18 |
| Standard for Markergene™
Carbohydrate Analysis Detection Kit M0272. |
|
| M1074 |
Eosin 5-isothiocyanate (EITC) |
60520-47-0 |
25 mg |
48.62 |
38.89 |
| Can be used as a reversible inhibitor of the erythrocyte calcium pump as well as an effective phosphorescent probe in protein rotational studies. |
|
| M1202 |
Eosin-5-thiouredylphenylboronic acid, triethylammonium salt |
N/A |
25 mg |
83.74 |
66.99 |
| Long-wavelength fluorescent labeling agent for cis-diols (sugars) at high pH values on cell surfaces and in vitro for carbohydrate determination in glycoproteins and for HPLC analysis of sugars. |
|
| M1282 |
Ethidium bromide |
1239-45-8 |
100 mg |
40.53 |
32.42 |
| Commonly used nucleic acid stain. |
|
| M1093 |
Ethidium homodimer (EthD-1) |
61926-22-5 |
1 mg |
133.14 |
106.51 |
| High affinity fluorescent nucleic stain. It binds to both DNA and RNA in a sequence-independent manner and with a >30-fold fluorescence enhancement. |
|
| M0255 |
MarkerGene™ FACS Fluorescent Blue lacZ β-Galactosidase Detection Kit |
|
1 kit |
200.03 |
160.02 |
| Allows highly sensitive detection of β-galactosidase activity in mammalian, yeast, bacterial, or plant cells, at a shorter wavelength (blue fluorescence) than other kits. This assay is especially amenable to co-staining with a green (fluorescein, GFP) labeling for dual labeling experiments. |
|
| M0955 |
MarkerGene™ FITC Antibody Labeling Kit |
|
1 kit |
182.70 |
146.16 |
| Provides reagents and methods to simply and quickly label antibodies or other proteins with the brilliant green dye fluorescein. |
|
| M1445 |
Fluo-3 |
123632-39-3 |
|
|
|
| Calcium chelator and indicator. |
|
| M1513 |
Fluo-3 AM |
121714-22-5 |
1 mg |
256.83 |
205.46 |
| Cell permeable fluorescent indicator for intracellular calcium. It is non-fluorescent until hydrolyzed intracellularly. |
|
| M0250 |
Fluorescein di-β-D-galactopyranoside (FDG) |
17817-20-8 |
5 mg |
86.88 |
69.50 |
| Ultra-sensitive substrate for lacZ β-galactosidase detection in vivo and in vitro. |
|
| M0881 |
Fluorescein di-β-D-glucopyranoside (FDGlu) |
129787-66-2 |
5 mg |
91.22 |
72.98 |
| Ultrasensitive fluorogenic substrate for measurement of β-glucosidase activity in vivo or in vitro. |
|
| M0969 |
Fluorescein di-β-D-glucuronide, dimethyl ester |
N/A |
5 mg |
106.95 |
85.56 |
| Sensitive cell-permeant β-glucuronidase substrate for live cell and tissue assays. |
|
| M0308 |
Fluorescein di-β-D-N-acetylgalactosamine |
N/A |
5 mg |
106.95 |
85.56 |
| Useful ultrasensitive fluorescent substrate for galactosaminidase or hexosaminidase activity. |
|
| M0060 |
Fluorescein diacetate (FDA) |
596-09-8 |
1 g |
48.62 |
38.89 |
| Most widely-used green fluorogenic substrate for esterase activity. |
|
| M1034 |
Fluorescein diphosphate, tetraammonium salt |
217305-49-2 |
5 mg |
218.82 |
175.06 |
| Most widely used ultrasensitive fluorogenic substrate for alkaline phosphatases. |
|
| M0064 |
Fluorescein mono-β-D-galactopyranoside (FMGal) |
102286-67-9 |
5 mg |
133.75 |
107.00 |
| Convenient β-galactosidase fluorescent probe. Especially useful for enzymatic analyses where 1st order kinetics are desired. |
|
| M0065 |
Fluorescein mono-β-D-glucopyranoside (FMGlc) |
129787-63-9 |
10 mg |
66.87 |
53.50 |
| Highly fluorescent β-glucosidase probe for monitoring β-glucosidase activity where 1st order kinetics are desired. |
|
| M1040 |
Fluorescein mono-β-D-N-acetylgalactosamine |
N/A |
5 mg |
80.27 |
64.22 |
| Useful fluorescent substrate for galactosaminidase or hexosaminidase activity. |
|
| M1485 |
Fluorescein mono-β-D-N-Acetylglucosamine |
148806-95-5 |
5 mg |
80.27 |
64.22 |
| Fluorogenic substrate for hexosaminidase enzymes. Has been used for enzymatic characterization of O-GlcNAcase isoforms. |
|
| M0204 |
Fluorescein, high purity standard |
2321-07-5 |
100 mg |
24.36 |
19.49 |
| Widely used green fluorescent fluorophore. |
|
| M0979 |
Fluorescein-5(6)-carboxamidocaproic acid N-hydroxysuccinimidyl ester |
114616-31-8 |
5 mg |
76.00 |
60.80 |
| Useful fluorescent amine-reactive probe has an added spacer to improve accessibility of the reactive group by nucleophiles in preparation of bioconjugates. |
|
| M1036 |
Fluorescein-5-thiosemicarbazide |
76863-28-0 |
50 mg |
72.98 |
58.38 |
| Cell impermeant fluorescent probe useful for determining protein and peptide topology on the cell surface. |
|
| M1222 |
MarkerGene™ Fluorescent Alkaline Phosphatase Assay Kit |
|
1 kit |
247.17 |
197.74 |
| Allows fast and easy measurement of alkaline phosphatase activity in vitro, in cell preparations or in vivo using the substrate fluorescein diphosphate. |
|
| M1460 |
MarkerGene™ Fluorescent Bacterial Detection and Quantification Kit |
|
1 kit |
221.29 |
177.03 |
| Employs the highly sensitive substrate fluorescein di-β-D-galactopyranoside (FDG, M0250),
a di-β-galactoside conjugate of the dye fluorescein to more effectively detect E. Coli and other coliforms through both basal and induced β-galactosidase activity.
|
|
| M1245 |
MarkerGene™ Fluorescent Cellulase Assay Kit |
|
1 kit |
240.19 |
192.15 |
| Allows fast and easy detection of most cellulases in a microtiter plate based assay format. |
|
| M0612 |
MarkerGene™ Fluorescent Lipase Assay Kit |
[102807-51-2] for substrate reagent |
1 kit |
266.44 |
213.15 |
| Allows fast and easy measurement of lipase activity in vitro, in cell preparations or in vivo using the fluorescent fatty acid substrate 1,2-dioleoyl-3-(pyren-1-yl)decanoyl-rac-glycerol M0258. |
|
| M1319 |
MarkerGene™ Fluorescent Protease Assay Kit |
|
1 kit |
170.10 |
136.08 |
| Allows quick and simple measurement of general protease activity in biological samples. |
|
| M0174 |
5-Fluorouridine 5'-O-β-D-galactopyranoside |
149965-92-4 |
5 mg |
106.95 |
85.56 |
| Analog of 5-fluorouridine (5-FUR) useful for lacZ specific release of 5-FUR in recombinant cells or tissues. |
|
| M0350 |
N-FMOC Rhodamine 110 |
293313-27-6 |
10 mg |
66.87 |
53.50 |
| Mono-protected rhodamine 110 derivative useful for the synthesis of heterofunctional R110 derivatives, including peptidase substrates. |
|
| M0802 |
6-FMOC-Amino-D-luciferin |
578705-74-2 |
5 mg |
187.23 |
149.78 |
| Amino analog of D-luciferin substrate for firefly luciferase activity. Useful in automated peptide synthesis for incorporation of amino-luciferin into synthetic peptides. |
|
| M1352 |
MarkerGene™ β-Galactosidase Staining Kit |
|
1 kit |
144.38 |
115.50 |
| Can be used to monitor and detect enzyme activity in stably or transiently transfected
cells or transgenic tissues using the sensitive chromogenic substrate 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside
(X-Gal). |
|
| M0259 |
MarkerGene™ in vivo lacZ β-Galactosidase Intracellular Detection Kit |
|
1 kit |
200.03 |
160.02 |
| Allows ultra-sensitive detection of β-galactosidase activity in live mammalian, yeast, bacterial, or plant cells. Especially useful for FACS analysis. |
|
| M0276 |
MarkerGene™ β-Galactosidase Sample Kit |
|
1 kit |
266.44 |
213.15 |
| This kit consists of samples of several of our most popular β-galactosidase substrates and their reference fluorophores allowing multiplexed analysis of lacZ β-galactosidase activity at a variety of wavelengths. This kit is perfect for those occasions where the preferred wavelength of detection is under development. |
|
| M0095 |
alpha-DGalU(1-4)DGalU |
5894-59-7 |
10 mg |
45.55 |
36.44 |
| Digalacturonic acid disaccharide prepared by action of polygalacturonidase upon pectin, and purified by column chromatography. This disaccharide is a useful starting material for synthesis of oligosaccharides and antigens. |
|
| M1193 |
MarkerGene™ Gaussia Luciferase Cellular Assay Kit |
|
1 kit |
71.61 |
57.29 |
| Gaussia luciferase is a new reporter gene isolated from the marine copepod Gaussia princeps, and can be expressed in mammalian cells using commercially available reporter plasmids. This luciferase, which does not require ATP, catalyzes the oxidation of the substrate (M0739) in a reaction that emits light (at 470 nm), and has considerable advantages over other reporter genes. |
|
| M0877 |
MarkerGene™ β-Glucuronidase (GUS) Reporter Gene Activity Detection Kit |
|
1 kit |
242.81 |
194.25 |
| Allows for quantitative measurement of GUS enzyme activity in transformed plants through use of a fluorogenic substrate. |
|
| M1025 |
4-Hydrazino-7-nitrobenzofurazane |
90421-78-6 |
25 mg |
53.48 |
42.78 |
| Fluorescent derivatization reagent useful for aliphatic aldehyde detection including reducing carbohydrates. |
|
| M0794 |
MarkerGene™ Hydrophobic Protein Analysis Kit |
|
1 kit |
228.38 |
182.70 |
| Allows fast and easy measurement of proteins in complex mixtures using either solution analysis or 1D- or 2D-SDS-PAGE analysis. Both denatured or native conformations of proteins isolated from cell preparations can be analyzed. |
|
| M1280 |
7-Hydroxycoumarin |
93-35-6 |
100 mg |
35.44 |
28.35 |
| Blue fluorophore standard. |
|
| M1236 |
8-Hydroxypyrene-1,3,6-trisulfonic acid trisodium salt |
6358-69-6 |
50 mg |
24.78 |
19.82 |
| Highly water soluble, brightly fluorescent dye useful as a fluorescent pH indicator with emission change in the physiological pH range and as a dye in optical sensor applications. |
|
| M1111 |
2-Iminobiotin |
13395-35-2 |
10 mg |
29.40 |
23.52 |
| Reversible inhibitor of mouse inducible and rat neuronal nitric oxide synthase (iNOS/NOS II; nNOS/NOS I). Also useful for affinity purification of avidin on immobilized solid supports. |
|
| M0638 |
5-(Iodoacetamido)fluorescein |
63368-54-7 |
25 mg |
66.59 |
53.27 |
| Excellent thiol reactive fluorescein derivative for synthesis of fluorescently labeled organelles, proteins and peptides at or near their physiological pH values (pH 7) including enzymes (e.g. (Na, K)-ATPase) or organelles like nucleii and nuclear matrices. |
|
| M0093 |
Isethionyl acetimidate sodium salt hydrochloride |
52914-43-9 |
1 g |
26.80 |
21.44 |
| Cell membrane cross-linking reagent. |
|
| M1060 |
L-Leucine 7-amido-4-methyl coumarin hydrochloride (Leu-AMC.HCl) |
62480-44-8 |
25 mg |
110.91 |
88.73 |
| Sensitive fluorogenic substrate for leucine aminopeptidase which releases a bright blue fluorescent dye, 7-amino-4-methylcoumarin M1059 upon enzyme activity (excitation 380nm emission 440nm). |
|
| M1049 |
MarkerGene™ Live Cell Fluorescent Reactive Oxygen Species Detection Kit |
|
1 kit |
200.97 |
160.78 |
| Provides all necessary reagents, buffers and a detailed protocol for the detection of Reactive Oxygen Species (ROS, peroxidase activity) in live cells. |
|
| M1045 |
MarkerGene™ Live Cell Glutathione Transferase Activity Kit |
|
1 kit |
160.55 |
128.44 |
| Provides reagents and methods to simply and quickly measure glutathione transferase activity in live cells, tissues or cell lysate samples |
|
| M0626 |
MarkerGene™ Live Cell Luciferase Assay Kit |
|
1 kit |
106.58 |
85.26 |
| Contains synthetic D-luciferin, the primary substrate of the firefly luciferase light producing system, as well as a luciferase assay buffer |
|
| M0795 |
MarkerGene™ Live:Dead /Cytotoxicity Assay Kit |
|
1 kit |
200.97 |
160.78 |
| Allows fast and easy measurement of both living and dead cells by measuring intracellular esterase activity (live cells) and staining of nucleic acids in chromatin (dead cells) in vitro, and in cell culture. |
|
| M1214 |
MarkerGene™ Long Wavelength Fluorescent Lipase Assay Kit |
|
1 kit |
266.44 |
213.15 |
| Allows fast and easy measurement of lipase activity in vitro, in cell preparations or in vivo using the long wavelength substrate resorufin oleate. |
|
| M0237 |
D-Luciferin |
2591-17-5 |
5 mg |
76.00 |
60.80 |
| Primary substrate of the firefly luciferase light producing system. |
|
| M1087 |
D-Luciferin-6-O-β-D-galactopyranoside |
131474-38-9 |
5 mg |
157.89 |
126.31 |
| Analog of D-Luciferin contains a β-galactoside attached at the 6-O-position, and thus is not a substrate for the firefly luciferase enzyme until the galactose is removed e.g., by β-galactosidase activity. As such it represents an ultrasensitive substrate for chemiluminescent measurement of galactosidase activity in homogeneous assays, or in cell lysate samples when the enzyme luciferase is added. |
|
| M0236 |
D-Luciferin, 6'-methyl ether |
3022-11-5 |
5 mg |
100.25 |
80.20 |
| Inhibitor of the firefly luciferase light producing system. Useful in chemiluminescent dual assay systems with both luciferase and cytochrome P450 enzymes. |
|
| M0906 |
D-Luciferin, ethyl ester |
135251-85-3 |
10 mg |
60.80 |
48.64 |
| Membrane permeable analog of the primary substrate for the firefly luciferase light producing system with 30% higher light intensity. |
|
| M1573 |
RubyGlowTM Luminescent Bacterial Detection Kit |
|
1 kit |
110.00 |
|
| The new RubyGlowTM Luminescent Bacterial Detection Kit can detect bacteria from a number of media or sources with an extremely high sensitivity (low detection limit). In addition, extended incubation (2-4 hours) of samples at 37ºC can significantly lower the detection limit and increase sensitivity using a protocol that can be completed in one day. Data variation is low and confidence is high compared with several current “Add and Read” commercial kits. Due to its stable light emission, we have adapted this kit for use in a microtiter plate format; thus enabling the processing of a large number of samples (such as food or clinical samples) at the same time. This microplate format assay is amenable to high throughput (HTS) applications for analysis of bacterial susceptibility and antibiotic screening. For more information contact us at techservice@markergene.com. |
|
| M1376 |
MarkerGene™ LysoLive™ Lysosomal Phosphatase Assay Kit |
|
1 kit |
331.80 |
265.44 |
| Useful for labeling lysosomes in a live-cell format and capable of monitoring lysosomal metabolic activity. |
|
| M1377 |
MarkerGene™ LysoLive™ Lysosomal Sulfatase Assay Kit |
|
1 kit |
331.80 |
265.44 |
| Useful for labeling lysosomes in a live-cell format and capable of monitoring lysosomal metabolic activity. |
|
| M1327 |
pCdgCAT Mammalian CAT Expression Vector |
N/A |
20 ug |
102.80 |
102.80 |
| High copy number eukaryotic vector, pCdgCAT expresses the E. coli Chloramphenicol Acetyltransferase (CAT) gene under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. Very useful for transfection of mammalian cells in culture and for use in other species. |
|
| M0951 |
pCMVβ Mammalian lacZ Expression Vector |
N/A |
20 ug |
127.05 |
127.05 |
| High copy number eukaryotic vector, pCMVβ expresses the full-length β-galactosidase gene under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. Very useful for transfection of mammalian cells in culture and for use in other species. |
|
| M0952 |
pSV40β Mammalian lacZ Expression Vector |
N/A |
20 ug |
127.05 |
127.05 |
| Common eukaryotic expression vector, pSV40β, expresses the full-length β-galactosidase gene under the control of simian virus 40 (SV40) early promoter. Very useful for transfection of mammalian cells in culture and for use in other species. |
|
| M1015 |
pCMVβ Mammalian lacZco Expression Vector |
N/A |
20 ug |
261.03 |
261.03 |
| High copy number eukaryotic vector, pCMVβ expresses the full-length codon-optimized β-galactosidase gene (lacZco) under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. |
|
| M1016 |
pSV40β Mammalian lacZco Expression Vector |
N/A |
20 ug |
261.03 |
261.03 |
| Common eukaryotic expression vector, pSV40β expresses the full-length codon-optimized β-galactosidase gene (lacZco) under the control of simian virus 40 (SV40) early promoter. |
|
| M1017 |
pCMVβ Mammalian lacZnls12co Expression Vector |
N/A |
20 ug |
273.74 |
273.74 |
| High copy number eukaryotic vector, pCMVβ expresses the full-length codon-optimized β-galactosidase gene (lacZco) with an effective nuclear localization signal under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. The nls12 variant results from the addition of a twelve amino acid sequence, ProLysLysLysArgLysValGluAspProLysAsp (from the SV40 T antigen nuclear localization signal) after the methionine initiation residue. |
|
| M1018 |
pSV40β Mammalian lacZnls12co Expression Vector |
N/A |
20 ug |
273.74 |
273.74 |
| Eukaryotic expression vector, pSV40β expresses the full-length codon-optimized β-galactosidase gene (lacZco) under the control of simian virus 40 (SV40) early promoter. The nls12 variant results from the addition of a twelve amino acid sequence, ProLysLysLysArgLysValGluAspProLysAsp (from the SV40 T antigen nuclear localization signal) after the methionine initiation residue. |
|
| M1306 |
pCMVLuxA Mammalian LuxA Expression Vector |
N/A |
20 ug |
117.81 |
117.81 |
| High copy number eukaryotic vector, pCMVLuxA, expresses the alpha subunit of luciferase encoded by the bacterium Vibrio harveyi.
When paired with pCMVLuxB M1307, luminescence can be produced in mammalian cell systems. |
|
| M1307 |
pCMVLuxB Mammalian LuxB Expression Vector |
N/A |
20 ug |
117.81 |
117.81 |
| High copy number eukaryotic vector, pCMVLuxB, expresses the beta subunit of luciferase encoded by the bacterium Vibrio harveyi. When paired with pCMVLuxA M1306, luminescence can be produced in mammalian cell systems. |
|
| M1389 |
MarkerGene™ Cellular Senescence Assay Kit |
|
1 kit |
202.65 |
162.12 |
| Can be used to monitor and detect β-galactosidase activity in senescent cells at pH 6.0 using X-Gal. |
|
| M0271 |
MarkerGene™ Membrane Fluidity Kit |
|
1 kit |
118.13 |
94.50 |
| Allows quantitative monitoring of membrane fluidity in cell membranes, micelles, and vesicles through use of a lipophilic pyrene probe. |
|
| M0540 |
meso-Tetraphenylporphyrin 99+% |
917-23-7 |
1 g |
121.54 |
97.23 |
| Sensitizer used for the photochemical generation of singlet oxygen. Many metal complexes have been made. |
|
| M1231 |
7-Methoxycoumarin |
531-59-9 |
50 mg |
61.45 |
49.16 |
| Fluorogenic Cytochrome P450 and microsomal dealkylase substrate for several isoforms of these enzymes with blue fluorescence emission. |
|
| M1021 |
7-Methoxycoumarin-3-carboxylic acid |
20300-59-8 |
100 mg |
53.48 |
42.78 |
| Amine-reactive blue fluorescent probe provides a distinguishable contrast when used with longer-wavelength probes in certain multicolor fluorescence applications such as nucleic acid and protein microarrays, in situ hybridization and immunofluorescence. |
|
| M0121 |
Methyl purple, sodium salt |
209187-17-7 |
500 mg |
80.27 |
64.22 |
| Redox indicator, purple to clear upon oxidation. Useful indicator of bacterial growth or plant redox mechanisms. |
|
| M1388 |
4-Methylumbelliferyl sulfate, potassium salt |
15220-11-8 |
25 mg |
30.85 |
24.68 |
| Highly sensitive fluorescent substrate for monitoring aryl sulfatase activity. |
|
| M0241 |
4-Methylumbelliferyl β-D-galactopyranoside (MUG) |
6160-78-7 |
1 g |
53.16 |
42.52 |
| Bright blue fluorogenic β-galactosidase substrate, especially useful for in vitro assays. |
|
| M0240 |
4-Methylumbelliferyl β-D-glucuronide (MUGlcU) |
6160-80-1 |
25 mg |
36.44 |
29.15 |
| Common blue fluorogenic GUS substrate for measuring activity in plant lysate, homogenate, leaf disk or whole plant assays. |
|
| M1065 |
4-Methylumbelliferyl 4-guanidinobenzoate hydrochloride hydrate |
34197-46-1 |
100 mg |
18.27 |
14.62 |
| Spectrofluorometric titrant for chymotrypsin, trypsin, thrombin, and factor Xa. |
|
| M0554 |
4-Methylumbelliferyl β-D-lactoside |
84325-23-5 |
25 mg |
83.24 |
66.59 |
| Fluorescent substrate for β-lactosidase or galactosidase and glucosidase activities; releases 4-methylumbelliferone (EX 385 nm, EM 502 nm, e = 16K) upon enzyme action. The fluorescence is optimal at pH values above 8-9. |
|
| M0270 |
Monofluoresceinated mannitol (MFM) |
N/A |
10 mg |
133.98 |
107.18 |
| Has been used in labeling of the interstitial (intercellular) spaces between neurons in vitro. |
|
| M1580 |
MarkerGene™ Multiple Drug Resistance Microtiterplate Assay Kit |
|
|
210.00 |
168.00 |
| This kit contains protocols and reagents for use in the detection of Multiple Drug Resistance within cells. |
|
| M0326 |
Myo-inositol galactoside |
16908-86-4 |
5 mg |
100.25 |
80.20 |
| Galactoside derivative of myo-inositol will release myo-inositol upon action of β-galactosidase. |
|
| M1314 |
Naphthofluorescein |
61419-02-1 |
100 mg |
39.06 |
31.25 |
| Near infrared dye is also a cell membrane permeable inhibitor of furin (IC50=12µM). |
|
| M0239 |
N-(NBD-Aminohexanoyl) Sphingosine |
86701-10-2 |
1 mg |
147.00 |
117.60 |
| NBD-ceramide analog used to detect, at the cellular level, a variety of lysosomal storage diseases as well as intracellular distribution and transport of the labeled sphingolipid molecules in living cells. |
|
| M0500 |
NBD-X, SE |
145195-58-0 |
25 mg |
133.66 |
106.93 |
| Fluorescent dye used to label amines. |
|
| M1441 |
Nile Red |
7385-67-3 |
10 mg |
58.28 |
46.62 |
| Lipophilic dye that stains intracellular lipid droplets to produce a bright red fluorescence in live cells. |
|
| M1410 |
4-Nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole |
139332-66-4 |
10 mg |
44.10 |
35.28 |
| Widely used fluorophore that has the unusual environmentally sensitive property of increasing fluorescence in low pH environments. |
|
| M1475 |
Marker Gene™ Non-Radioactive Cell Proliferation Assay Kit |
|
1 Kit |
235.20 |
188.16 |
The MarkerGene™ Non-Radioactive Cell Proliferation Assay Kit uses a common tetrazolium dye MTT ((3-(4,5-imethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) as a method of quickly and accurately measuring cell viability. The assay relies on the reduction by live cells of the tetrazolium salt MTT by intracellular oxidoreductases. It is therefore widely regarded as an accurate indicator of cell redox activity.
|
|
| M1073 |
5-(Octadecanoylamino)fluorescein |
110698-53-8 |
100 mg |
86.86 |
69.49 |
| Amphiphilic fluorescent probe useful in monitoring membrane lipid behavior and fluorescence resonance energy transfer applications. |
|
| M1076 |
10-Octadecylacridine orange bromide |
75168-16-0 |
20 mg |
106.95 |
85.56 |
| Useful fluorescent mitochondrial membrane probe used to analyze mitochondria by flow cytometry and measure changes in mitochondrial mass during apoptosis. |
|
| M1227 |
8-Octadecyloxypyrene-1,3,6-trisulfonic acid trisodium salt |
111024-84-1 |
10 mg |
139.64 |
111.71 |
| Highly water soluble polar fluorescent lipid probe for the sensitive fluorimetric assay of cationic surfactants. |
|
| M1296 |
8-Octanoyloxypyrene-1,3,6-trisulfonic acid trisodium salt |
115787-84-3 |
25 mg |
61.56 |
49.24 |
| Highly polar, highly water soluble lipase substrate is a derivative of HPTS (pyranine) that forms tight micelles in aqueous solutions. |
|
| M1228 |
Octyl β-D-1-thioglucopyranoside |
85618-21-9 |
500 mg |
42.11 |
33.68 |
| Non-ionic detergent for purification of soluble proteins from cells, including bacteria, and for solubilization and reconstitution of membrane proteins.
|
|
| M0224 |
1-O-Oleoyl-2-O-acetyl-3-O-β-D-galactopyranosyl syn-glycerol |
N/A |
5 mg |
80.27 |
64.22 |
| Synthetic diglyceride analog is an activator of protein kinase C, that is released upon action of β-galactosidase in lacZ transfected cells and tissues. |
|
| M0338 |
Pantothenic acid 2,4-di-O-β-D-galactopyranoside |
137-08-6 ( pantothenic acid aglycone) |
10 mg |
167.13 |
133.71 |
| Analog of pantothenic acid (vitamin B5) releases pantothenic acid upon action of β-galactosidase enzyme activity. |
|
| M1394 |
pDC57 Mammalian Luciferase Expression Vector |
N/A |
20 ug |
250.95 |
250.95 |
| High copy number eukaryotic vector, pDC57, expresses a mutant luciferase gene under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. This vector encodes a mutant enzyme that catalyzes the production of long wavelength (red) light (Em:605) from D-luciferin, M0237. |
|
| M1395 |
pDC99 Mammalian Luciferase Expression Vector |
N/A |
20 μg |
250.95 |
250.95 |
| High copy number eukaryotic vector, pDC99 expresses a mutant luciferase gene under the control of the Simian virus 40Early Core (SV40) promoter. This vector encodes a mutant enzyme that catalyzes the production of long wavelength (red) light (Em:605) from D-luciferin, M0237. |
|
| M0261 |
Phenethyl-1-thio- β-D-glucopyranosiduronic acid (PETGU) |
N/A |
10 mg |
33.38 |
26.70 |
| β-Glucuronidase inhibitor similar to PETG. |
|
| M0260 |
Phenethyl-1-thio- β-D-glucopyranoside (PETGlc) |
800376-82-3 |
10 mg |
33.38 |
26.70 |
| β-Glucosidase inhibitor similar to PETG. |
|
| M1091 |
N,N,N-Trimethyl-4-(6-phenyl-1,3,5-hexatrien-1-yl)phenylammonium p-toluenesulfonate (TMA-DPH) |
115534-33-3 |
5 mg |
48.62 |
38.89 |
| Fluorescent probe for membrane fluidity measurements. Especially useful in the study of monolayer dynamics of lipoproteins and similar analogous systems. |
|
| M1088 |
1-[4-(Dimethylamino)phenyl]-6-phenylhexatriene (DMA-DPH) |
79849-61-9 |
100 mg |
160.43 |
128.34 |
| Useful fluorescent probe of lipid bilayer structure and dynamics. Used to monitor cell membrane interiors. |
|
| M0529 |
3-Phenylumbelliferone |
6468-96-8 |
50 mg |
36.44 |
29.15 |
| pH indicator for use in the physiological range (pKa 7.8) |
|
| M0541 |
3-Phenylumbelliferone 7-O-phosphate hemipyridinium salt |
97970-92-8 |
50 mg |
72.98 |
58.38 |
| Alkaline phosphatase substrate which releases the bright green fluorescent dye, 3-phenylumbelliferone, at the site of enzyme activity (EX = 383 nm ; EM = 472 nm). |
|
| M1390 |
pNosdcGUS Plant GUS Expression Vector |
N/A |
20 μg |
252.00 |
252.00 |
| High copy number plant vector, pNosdcGUS, expresses the GUS gene under the control of the nopaline synthase (pNos) promoter. This vector may be used to monitor virus production and transfection efficiency. |
|
| M0793 |
Propidium iodide |
25535-16-4 |
150 μL |
54.74 |
43.79 |
| Excellent counterstain for DNA and chromatin, providing a good contrast when also staining with green-fluorescent antibodies, or substrates. |
|
| M0524 |
Pyrene 1-sulfonyl chloride |
61494-52-8 |
25 mg |
76.00 |
60.80 |
| Useful amine reactive reagent for labeling proteins and peptides. The fluorescence emission is environment dependent. |
|
| M0535 |
Pyrene 8-hydroxy-1, 4, 6-trisulfonyl chloride |
N/A |
10 mg |
113.65 |
90.92 |
| Useful for labeling amine groups of proteins, peptides or other compounds (drugs), and has unusual pH dependent fluorescence properties and pKa changes, after conjugation. Often called a "photoacid" because it can be transformed by light from a weak acid into a strong acid. |
|
| M0525 |
Pyrene N-hexadecylsulfonamide |
N/A |
25 mg |
80.27 |
64.22 |
| Polar, neutral, blue-fluorescent probe for labeling of membranes in vivo. |
|
| M0274 |
1-Pyrenedecanoic acid (PDA) |
64701-47-9 |
10 mg |
60.77 |
48.62 |
| Fluorescent lipid analog that can be used to label membranes in vivo and to measure membrane fluidity. |
|
| M0330 |
Pyridoxal galactoside |
[65-22-5] for pyridoxal aglycone |
2 mg |
93.55 |
74.84 |
| Pyridoxine (vitamin B6) analog is a useful tissue culture media component that will release pyridoxal upon action of lacZ β-galactosidase. Useful as a media component for selection of lacZ positive cells in culture. |
|
| M0304 |
Pyridoxine galactoside |
[58-56-0] for pyridoxine aglycone |
10 mg |
120.35 |
96.29 |
| Pyridoxine (vitamin B6) analog is a useful tissue culture component that will release pyridoxine upon action of lacZ β-galactosidase. |
|
| M0202 |
Resorufin |
635-78-9 |
20 mg |
18.17 |
14.53 |
| Red fluorophore standard. Widely used in enzyme assay systems. |
|
| M0203 |
Resorufin β-D-galactopyranoside (Res-Gal) |
95079-19-9 |
10 mg |
36.33 |
29.06 |
| Red fluorogenic substrate for lacZ β-galactosidase activity, intracellularly, or in vitro. |
|
| M1037 |
Resorufin β-D-N-acetylgalactosamine |
N/A |
10 mg |
80.27 |
64.22 |
| Useful fluorescent substrate for galactosaminidase or hexosaminidase activity. The fluorescent product, resorufin, has Abs 570nm / Em 585 nm. |
|
| M1166 |
Resorufin 7-O-methyl ether |
5725-89-3 |
5 mg |
112.88 |
90.30 |
| One of the most popular substrates for analysis of microsomal dealkylase and cytochrome P450 enzymes. |
|
| M0806 |
Resorufin acetate |
1152-14-3 |
25 mg |
66.29 |
53.03 |
| Common long-wavelength esterase substrate, useful in cell viability assays, or for high throughput screening of esterase activity in a live cell format. |
|
| M1238 |
Resorufin Cellobioside |
N/A |
5 mg |
92.63 |
74.11 |
| Fluorogenic substrate for detection of cellulase activity. |
|
| M1209 |
Resorufin ethyl ether |
5725-91-7 |
5 mg |
123.25 |
98.60 |
| Microsomal dealkylase and Cytochrome P450 enzyme substrate. Releases the red fluorophore resorufin (Abs. 571 nm; Em. 585 nm) upon enzyme reaction. Routinely used to differentiate the various cytochrome P450 isoforms. |
|
| M0569 |
Resorufin β-D-glucopyranoside |
101490-85-1 |
10 mg |
26.57 |
21.26 |
| Stable red fluorogenic substrate for β-glucosidase activity. |
|
| M1312 |
Resorufin maltotriose |
N/A |
5 mg |
92.63 |
74.11 |
| Resorufin maltotriose is an α-amylase substrate with red emission (λ max=585nm) after enzyme reaction. |
|
| M1208 |
Resorufin oleate |
N/A |
10 mg |
79.23 |
63.39 |
| Fluorogenic substrate with red emission for use in detection of intracellular and in vitro lipase activity. |
|
| M1207 |
Resorufin-7-O-phosphate, diammonium salt (Res-Phos) |
N/A |
0 |
0.00 |
0.00 |
| Phosphatase substrate, (Ex/Em=571/583nm upon dephosphorylation), can be used to quantify phosphatase activity in solutions, in cell extracts, in live cells as well as on solid surfaces (such as PVDF membranes) |
|
| M1340 |
Resorufin-α-D-mannopyranoside |
N/A |
5 mg |
128.10 |
102.48 |
| Red fluorogenic substrate can be used to monitor intracellular mannosidase activity using a continuous assay format. Activity has been evaluated for cloned human Golgi α-Mannosidase II as well as human lysosomal α-mannosidase.
|
|
| M1281 |
Rhodamine 110 |
13558-31-1 |
25 mg |
30.03 |
24.02 |
| Red fluorophore standard. Widely used in enzyme assay systems. |
|
| M0542 |
Rhodamine 123 |
62669-70-9 |
25 mg |
53.16 |
42.52 |
| Cell-permeant, green fluorescent dye is used in many applications including bacterial viability and vitality assays, mitochondrial membrane potential assays in apoptotic cells, and sorting respiratory-deficient yeast cells using flow cytometry. |
|
| M1224 |
Rhodamine B octadecyl ester perchlorate |
142179-00-8 |
10 mg |
94.37 |
75.50 |
| Lipophilic energy transfer acceptor from lipophilic fluoresceins in fluorescence energy transfer (FRET) assays in cell fusion experiments. Also used as a probe for measuring potassium in fiber-optic sensors.
|
|
| M0370 |
Rhodol |
3086-44-0 |
5 mg |
80.27 |
64.22 |
| Highly fluorescent dye useful for oligonucleotide, antibody and protein labeling studies and for the preparation of peptide and esterase substrates. |
|
| M0328 |
Riboflavin galactoside |
83-88-5 for riboflavin aglycone |
2 mg |
106.95 |
85.56 |
| Riboflavin is a common component of tissue culture media. Riboflavin galactoside can be used to select for lacZ transfected cells in culture. |
|
| M1277 |
Rose bengal (4,5,6,7-Tetrachloro-2',4',5',7'-tetraiodofluorescein disodium salt) |
632-69-9 |
25 mg |
22.52 |
18.02 |
| Iodinated and chlorinated derivative of fluorescein. Since it does not easily cross live cell membranes, it has been used to stain dead or degenerated epithelial cells of the cornea and conjunctiva (including nuclei and cell walls) producing a red color. |
|
| M0780 |
Rose bengal diacetate |
61738-01-0 |
5 mg |
91.14 |
72.91 |
| Rose bengal is a per-halogenated fluorescein derivative that is among the most efficient producers of singlet oxygen known. This diacetate derivative exhibits greatly improved membrane permeability, allowing it to accumulate intracellularly where esterase activity releases the active rose bengal photosensitizer. |
|
| M1574 |
RubyGlowTM Luminescent Cell Proliferation Assay Kit |
|
1 kit |
110.00 |
|
| Most commercially available luciferase kits use green light emitting (562 nm) firefly analogs. The RubyGlowTM luminescent assay kits produce a red light (EM 619 nm) for measurement of cell number, cell proliferation, growth, viability or cytotoxicity based upon the generation of ATP in metabolically active cells. This unique feature enables the possibility of multiplexing. The new kit components involve no radioisotopes or toxic materials and are environmentally safe. Each kit provides enough reagents and a detailed protocol sufficient for 100 reactions in 96-well microplate format. |
|
| M1575 |
RubyGlowTM Luminescent Cell Viability Assay Kit |
|
1 kit |
110.00 |
|
| Most commercially available luciferase kits use green light emitting (562 nm) firefly analogs. The RubyGlowTM luminescent assay kits produce a red light (EM 619 nm) for measurement of cell number, cell proliferation, growth, viability or cytotoxicity based upon the generation of ATP in metabolically active cells. This unique feature enables the possibility of multiplexing. The new kit components involve no radioisotopes or toxic materials and are environmentally safe. Each kit provides enough reagents and a detailed protocol sufficient for 100 reactions in 96-well microplate format. |
|
| M1576 |
RubyGlowTM Luminescent Cytotoxicity Assay Kit |
|
1 kit |
110.00 |
|
| Most commercially available luciferase kits use green light emitting (562 nm) firefly analogs. The RubyGlowTM luminescent assay kits produce a red light (EM 619 nm) for measurement of cell number, cell proliferation, growth, viability or cytotoxicity based upon the generation of ATP in metabolically active cells. This unique feature enables the possibility of multiplexing. The new kit components involve no radioisotopes or toxic materials and are environmentally safe. Each kit provides enough reagents and a detailed protocol sufficient for 100 reactions in 96-well microplate format. |
|
| M1279 |
Sulforhodamine 101 |
60311-02-6 |
5 mg |
24.68 |
19.74 |
| Red fluorophore standard. Widely used in enzyme assay systems. |
|
| M1459 |
Sulforhodamine 101 cadaverine - TFA salt |
203866-87-9 |
5 mg |
141.75 |
113.40 |
| Texas Red™ Cadaverine is a useful labeling reagent for proteins and other biological molecules giving a stable, bright red fluorescence with excitation at 591 nm. |
|
| M1120 |
Sulforhodamine 101 sulfonyl chloride |
82354-19-6 |
5 mg |
68.72 |
54.98 |
| Reactive red fluorophore which couples covalently to amino groups on proteins and other compounds. Suitable for use with FITC in dual parameter flow cytometry studies. Equivalent to Texas Red™. |
|
| M0972 |
MarkerGene™ TAMRA Antibody/Protein/Cell Labeling Kit |
|
1 kit |
200.97 |
160.78 |
| Provides reagents and methods to simply and quickly label antibodies or other proteins with the amine-reactive dye 5(6)-carboxytetramethylrhodamine. |
|
| M1620 |
OliGlo™ TAMRA FISH Kit - trial size |
|
|
245.00 |
|
| Fluorescence in situ hybridization (FISH) is a cytogenetic technique used to detect and localize the presence or absence of specific DNA sequences on chromosomes. FISH uses fluorescent probes that bind to only those parts of the chromosome with which they show a high degree of sequence similarity. Fluorescence microscopy can be used to find out where the fluorescent probe is bound to the chromosome. FISH is often employed for discovery of specific features in DNA and is useful in genetic analysis, medicine, or species identification. FISH can also be used to detect and localize specific mRNAs within tissue samples. In this context, it can help define the spatial-temporal patterns of gene expression within cells and tissues.
The probes used in these analyses are typically short complimentary sequences to the target DNA which can be fluorescently labeled using this kit. In a typical protocol, metaphase chromosome spreads are prepared on glass slides, RNase treated, dehydrated and denatured. The slides are then hybridized with the fluorescently labeled DNA probes for 16-24 hours. Post hybridization, the slides are washed, counterstained with a 4’,6-diamidino-2-phenylindole (DAPI) / antifade mixture, and analyzed using fluorescence microscopy.
Our OliGlo™ kits directly label nucleic acid through reaction with the phosphate groups (terminal and backbone) of the probe nucleotide providing cleaner hybridization analysis.
|
|
| M1621 |
OliGlo™ TAMRA FISH Kit - standard size |
|
|
720.00 |
|
| Fluorescence in situ hybridization (FISH) is a cytogenetic technique used to detect and localize the presence or absence of specific DNA sequences on chromosomes. FISH uses fluorescent probes that bind to only those parts of the chromosome with which they show a high degree of sequence similarity. Fluorescence microscopy can be used to find out where the fluorescent probe is bound to the chromosome. FISH is often employed for discovery of specific features in DNA and is useful in genetic analysis, medicine, or species identification. FISH can also be used to detect and localize specific mRNAs within tissue samples. In this context, it can help define the spatial-temporal patterns of gene expression within cells and tissues.
The probes used in these analyses are typically short complimentary sequences to the target DNA which can be fluorescently labeled using this kit. In a typical protocol, metaphase chromosome spreads are prepared on glass slides, RNase treated, dehydrated and denatured. The slides are then hybridized with the fluorescently labeled DNA probes for 16-24 hours. Post hybridization, the slides are washed, counterstained with a 4’,6-diamidino-2-phenylindole (DAPI) / antifade mixture, and analyzed using fluorescence microscopy.
Our OliGlo™ kits directly label nucleic acid through reaction with the phosphate groups (terminal and backbone) of the probe nucleotide providing cleaner hybridization analysis.
|
|
| M1600 |
OliGlo™ TAMRA Universal Nucleic Acid Labeling Kit - trial size |
|
|
175.00 |
|
| Our OliGlo™ kits utilize a direct labeling methodology through reaction with the phosphate groups (terminal and backbone) on oligonucleotides, DNA or RNA. The active labeling reagents are prepared in situ from stable precursor molecules derived from a variety of highly fluorescent dyes and other detection labels, allowing the highly reactive labels to function at optimum efficiency for each sample. The OliGlo™ kits allow molecular biologists and clinical researchers to label or monitor genomic DNA or RNA samples, nucleotides or oligonucleotides for easy detection and quantification. |
|
| M1601 |
OliGlo™ TAMRA Universal Nucleic Acid Labeling Kit - standard size |
|
|
480.00 |
|
| Our OliGlo™ kits utilize a direct labeling methodology through reaction with the phosphate groups (terminal and backbone) on oligonucleotides, DNA or RNA. The active labeling reagents are prepared in situ from stable precursor molecules derived from a variety of highly fluorescent dyes and other detection labels, allowing the highly reactive labels to function at optimum efficiency for each sample. The OliGlo™ kits allow molecular biologists and clinical researchers to label or monitor genomic DNA or RNA samples, nucleotides or oligonucleotides for easy detection and quantification. |
|
| M0176 |
Tetracycline 10-O-β-D-galactopyranoside |
319426-63-6 |
2 mg |
106.95 |
85.56 |
| Tetracycline analog useful as an antibiotic for specific release in lacZ transfected cells and tissues. |
|
| M1722 |
Tetramethylrhodamine, ethyl ester |
|
50 uL |
66.50 |
53.20 |
| TMRE is a positively charged lipophilic fluorophore with red-orange fluorescence (EX 549; EM 575 nm) that rapidly accumulates in the mitochonria due to the relative negative charge of active mitochondria with respect to the cytosol. TMRE can also be used to monitor spontaneous transient depolarizations of mitochondria (Δψm), sometimes called mitochondrial flickers most commonly attributed to transient activation of the mitochondrial permeability transition pore (PTP). TMRE can therefore serve as a “Nernstian” indicator of Δψm, with mitochondrial depolarization resulting in a loss of the dye from the mitochondrion and a decrease in mitochondrial fluorescence intensity. |
|
| M0351 |
Thiamine galactoside |
N/A |
2 mg |
113.65 |
90.92 |
| Thiamine (vitamin B1) analog is a useful tissue culture component that will release thiamine upon action of lacZ β-galactosidase activity. Useful for selection of lacZ transfected cell lines in culture. |
|
| M0704 |
6-p-Toluidino-2-naphthalenesulfonic acid (TNS) |
7724-15-4 |
500 mg |
40.08 |
32.07 |
| Fluorescent probe for conformation and quantitation studies of proteins. Is practically nonfluorescent in water, becoming fluorescent when bound to membranes (q.y. ~0.27) or proteins (q.y. ~0.72). |
|
| M0720 |
6-p-Toluidino-2-naphthalenesulfonic acid, potassium salt (TNS-K+) |
32752-10-6 |
100 mg |
22.84 |
18.27 |
| Fluorescent probe for conformational studies and for use in quantitation of proteins. This potassium salt is more water soluble than the free acid, M0704. |
|
| M1295 |
Trifluoroacetamidoiminobiotin NHS ester |
N/A |
10 mg |
39.73 |
31.78 |
| Reversibly binds to avidin preventing protein denaturation during purification. Binds to avidin at pH > 9.5 and dissociates at pH 4.0. Forms a stable amide bond with primary amines at pH 7-9.
|
|
| M0522 |
4-Trifluoromethylumbelliferyl oleate |
352525-07-6 |
10 mg |
53.48 |
42.78 |
| Fluorescent substrate for use in detection of intracellular and in vitro lipase activity. |
|
| M0041 |
4-Trifluoromethylumbelliferone (TFMU) |
575-03-1 |
100 mg |
30.34 |
24.28 |
| Slightly longer wavelength analog of 4-methylcoumarin (4-MU) that also has a pKa that more closely matches physiological pH values. |
|
| M0252 |
4-Trifluoromethylumbelliferyl-β-D-galactopyranoside (TFMU-Gal) |
117153-55-6 |
25 mg |
106.95 |
85.56 |
| Blue-green fluorogenic β-galactosidase substrate that has a lower pKa for higher fluorescence in the physiological pH range. |
|
