pSV40Beta Mammalian lacZ Expression Vector

Product ID: M0952



Unit SizePriceQuantity 
20ug
$156.40

Availability: In stock


Description

Common eukaryotic expression vector, pSV40β, expresses the full-length β-galactosidase gene under the control of simian virus 40 (SV40) early promoter. Very useful for transfection of mammalian cells in culture and for use in other species where constitutive expression is desired.

The β-galactosidase enzyme expression is enhanced by elements including: SD/SA-RNA splice donor and acceptor sequence, and SV40 late polyadenylylation signal. pSV40β expression vector also contains β-lactamase, which acts as a selection marker (100 μg/ml ampicillin resistance) in E. coli host. See also our pCMVβ vector system, M0951.

Technical Data
SKU M0952
Unit Size 20ug
Notes

Full Length Sequence Data

Restriction Sites


References and Citations

References:

  • Hall, C.V. et al., 1983. "Expression and Regulation of Escherichia coli lacZ Gene Fusions in Mammalian Cells." J. Mol. Appl. Gen. 2:101.
  • Herbomel, P. et al., 1984. "Two distinct enhancers with different cell specificities coexist in the regulatory region of polyoma." Cell 39:653.
  • Nolan, G.P., et al., 1988. "Fluorescence-activated Cell Analysis and Sorting of Viable Mammalian Cells Based on b-D-galactosidase Activity after Transduction of Escherichia coli lacZ." Proc. Natl. Acad. Sci (USA). 85:2603.
  • Nikcevic, G. et al., 2003. "Improved transfection efficiency of cultured human cells." Cell Biol. Int. 27:735.
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