pSV40Beta Mammalian lacZnls12co Expression Vector

Product ID: M1018



Unit SizePriceQuantity 
20ug
$336.96

Availability: In stock


Description

Eukaryotic expression vector, pSV40β expresses the full-length codon-optimized β-galactosidase gene (lacZco) under the control of simian virus 40 (SV40) early promoter. The nls12 variant results from addition of a twelve amino acid sequence from SV40 T antigen nuclear location signal after the methionine initiation residue.

When expressed in mammalian cells, the codon-optimized gene results in expression levels of β-galactosidase 15-fold higher than those resulting from an analogous construct containing the native E. coli gene sequence. Enhanced transcript stability and increased translational efficiency provide for increased β-galactosidase expression, as suggested by RNA analysis. In addition, codon-optimization results in the elimination of several cryptic splice acceptor sites that are present in the native E. coli gene sequence and increases the amounts of un-spliced, full-length genomic RNA when used in a lentiviral vector containing a 5'-splice donor. The β-galactosidase enzyme expression is also enhanced by the SV40 late polyadenylylation signal. The pSV40β expression vector also contains β-lactamase, which acts as a selection marker (100 μg/ml ampicillin resistance) in E. coli host. If desired, the β-galactosidase codon optimized gene can be excised using XhoI and NotI sites to allow the insertion of other genes to be expressed under the same regulatory elements in mammalian cells. See also our pCMVβ lacZco vector system, Product M1017.

Technical Data
SKU M1018
Unit Size 20ug
Notes

Full Length Sequence Data

Restriction Sites



References and Citations

References:

  • Anson, D.S., Limberis, M., 2004. "An improved β-galactosidase reporter gene." J. of Biotech. 108:17-30.
  • Hall, C.V. et al., 1983. "Expression and Regulation of Escherichia coli lacZ Gene Fusions in Mammalian Cells.' J. Mol. Appl. Gen. 2:101.
  • Herbomel, P. et al., 1984. "Two distinct enhancers with different cell specificities coexist in the regulatory region of polyoma.' Cell 39:653.
  • Nolan, G.P., et al., 1988. "Fluorescence-activated Cell Analysis and Sorting of Viable Mammalian Cells Based on b-D-galactosidase Activity after Transduction of Escherichia coli lacZ." Proc. Natl. Acad. Sci (USA). 85:2603.
  • Nikcevic, G. et al., 2003. 'Improved transfection efficiency of cultured human cells." Cell Biol. Int. 27:735.
>Show more
>Show less

Click here for Newsletter articles about this product


Technical Support

Question about this product? Ask a Scientist!

We pride ourselves on the high quality of our products and want you to get the best possible results from your assays. If you have any questions about this product or need help optimizing your protocol check out the product FAQs below or ask your own question and one of our expert scientists will get back to you asap:


There are currently no frequently asked questions for this product, click the button above to ask a question