pTA-NfκB-luc Mammalian Transcription Factor Reporting Vector with Red Luciferase

Product ID: M2739



Unit SizePriceQuantity 
20 ug
$334.91

Availability: In stock


Description

A plasmid expression vector for monitoring NfκB activation via luciferase in mammalian cells.

This high copy number eukaryotic vector, pTA-NfκB-Luc Luciferase Reporter Vector expresses a mutant luciferase gene under the control of the minimal TA promoter with the cis-element for NfκB upstream. When NfκB is activated, it binds to cis-element causing the induction of luciferase gene expression. Luciferase activity is directly representative of NfκB activity. This luciferase encoded by this vector is a mutant enzyme that catalyzes the production of long wavelength (red) light (Em: 605) from D-Luciferin, Product M0237. This mutant luciferase is optimized for expression in mammalian cells, resulting in drastically improved light production, allowing detection of expression at extremely low levels. The mutant luciferase also has been shown to remain stable at 37°C over long time periods, a characteristic not seen in wild type luciferases The mutant luciferase enzyme expression is enhanced by the Kozak’s consensus sequence and SV40 late polyadenylation signal.

Technical Data
SKU M2739
Unit Size 20 ug
Detection Method Luminescence
Storage Conditions -20C

References and Citations

References:

  • Mamaev SV, Laikhter AL, Arslan T, Hecht SM. (1996) "Firefly luciferase: Alteration of the color of emitted light resulting from substitutions at position 286."
  • Ausubel F M, Brent R, Kingston RE, Moore DD, Seidman JG, Smith J A, Struhl K. (1994). Current Protocols in Molecular Biology (New York: Greene Publishing Associates and Wiley-Interscience).
  • Kajiyama N, Nakano E. (1993) "Thermostabilization of firefly luciferase by a single amino acid substitution at position 217." Biochemistry 32: 13795-1 3799.
  • Kajiyama N, Nakano E. (1991) "Isolation and characterization of mutants of firefly luciferase which produce different colors of light." Protein Eng. 4: 691.
  • Tatsumi H, Masuda T, Kajiyama N, Nakano E. (1989) "Luciferase cDNA from Japanese firefly, Luciola cruciata: cloning, structure and expression in Escherichia coli." J. Biolumin. Chemilumin. 3(2):75-78.
  • Felgner PL, Holm M, Chan H. (1989) “Cationic liposome mediated transfection.” Proc. West. Pharmacol. Soc. 32: 115-121.
  • Felgner PL, Ringold GM. (1989) “Cationic liposome-mediated transfection.” Nature 337: 387-388.
  • Landy A. (1989) ‘Dynamic, structural, and regulatory aspects of lambda site- specific recombination.” Annu. Rev. Biochem. 58: 913-949.
  • MacGregor GR, Caskey CT. (1989) “Construction of plasmids that express E. Coli beta-galactosidase in mammalian cells.” Nucleic Acids Res. 17: 2365.
  • Masuda T, Tatsumi H, Nakano E. (1989) "Cloning and sequence analysis of cDNA for luciferase of a Japanese firefly, Luciola cruciata." Gene. 77(2):265-270.
  • Chen C, Okayama H. (1987) “High-efficiency transformation of mammalian cells by plasmid DNA.” Molec. Cell. Biol. 7: 2745-2752.
  • Chu G, Hayakawa H, Berg P. (1987) “Electroporation for the efficient transfection of mammalian cells with DNA.” Nucleic Acids Res. 15: 1311-1326.
  • de Wet JR, Wood KV, Helinski DR, DeLuca M. (1985) "Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli." Proc Natl Acad Sci U S A. 82(23):7870-7873.
  • Southern PJ, Berg P. (1982) “Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.” J. Molec. Appl. Gen. 1: 327-339.
  • Miller JH. (1972). Experiments in Molecular Genetics (Cold Spring Harbor, New York: Cold Spring Harbor Laboratory).
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