Resorufin acetate

Product ID: M0806



Unit SizePriceQuantity 
25 mg
$81.59
  • Buy 5 for $65.27 each and save 21%

Availability: In stock


Description

Common long-wavelength esterase substrate, useful in cell viability assays, or for high throughput screening of esterase activity in a live cell format.

Resorufin acetate is a red fluorogenic esterase substrate that has also been used as a substrate for sheep liver cytosolic aldehyde dehydrogenase (CAD) as well as for assay of chymotrypsin activity, where the absorbance of the product resorufin M0202 is several times more intense than that formed by the widely used p-nitrophenyl acetate.

Technical Data
SKU M0806
CAS Number 1152-14-3
Unit Size 25 mg
Absorption 500nm
Emission Wavelength 593
Detection Method Fluorescence
Molecular Formula C₁₄H₉NO₄
Molecular Weight 255.23
Storage Conditions 2-8C, Desiccated

References and Citations

Citations:

  • Schmitt DM, Connolly KL, Jerse AE, Detrick MS, Horzempa J. (2016) "Antibacterial activity of resazurin-based compounds against Neisseria gonorrhoeae in vitro and in vivo." Int J Antimicrob Agents. pii: S0924-8579(16)30165-0.
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References:

  • Kitson, TM, Kitson KE, (1997) "Studies of the esterase activity of cytosolic aldehyde dehydrogenase with resorufin acetate as substrate." Biochem. J. 322: 701-708.
  • Kazlauskas, RJ (2006) "Quantitative Assay of Hydrolases for Activity and Selectivity Using Color Changesin Enzyme Assays",Jean-Louis Reymond, ed.,Wiley-VCH Verlag GmbH & Co. KGaA.
  • Bottcher D, Bornscheuer T, (2006) "High-throughput screening of activity and enantioselectivity of esterases." Nature Protocols 1: 2340-2343.
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