Resorufin β-D-glucopyranoside

Product ID: M0569



Unit SizePriceQuantity 
10mg
$32.71
  • Buy 5 for $26.17 each and save 20%

Availability: In stock


Description

Stable red fluorogenic substrate for β-glucosidase activity.

This stable β-glucosidase probe releases the red fluorescent dye resorufin M0202 upon β-glucosidase enzyme cleavage. Numerous applications in cell biology, high-throughput and high-content screening and diagnostics have been developed using this β-glucosidase probe.

Technical Data
SKU M0569
CAS Number 101490-85-1
Unit Size 10mg
Absorption 470nm
Extinction 18
Detection Method Fluorescence
Molecular Formula C₁₈H₁₇NO₈
Molecular Weight 375.33
Soluble In H₂O, DMSO
Storage Conditions -20C, Desiccated, Protect From Light
Notes Resorufin β-D-glucopyranoside can be used as a viability probe in nearly all types of cells since β-glucosidase activity is ubiquitous in virtually all cell types.

References and Citations

Citations:

  • Motabar O, Goldin E, Leister W, Liu K, Southall N, Huang W, Marugan JJ, Sidransky E, Zheng W. (2012) "A high throughput glucocerebrosidase assay using the natural substrate glucosylceramide." Anal Bioanal Chem. 402(2):731-9.
  • Chandrasekaran A, Bharadwaj R, Park JI, Sapra R, Adams PD, Singh AK. (2010) "A microscale platform for integrated cell-free expression and activity screening of cellulases." J Proteome Res. 9(11):5677-83.
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References:

  • Eggertson MJ, Craig DB. (1999) "b-galactosidase assay using capillary electrophoresis laser-induced fluorescence detection and resorufin-beta-D-galactopyranoside as substrate." Biomed. Chromatogr. 13, 516-519.
  • Hadd AG, Raymond DE, Halliwell JW, Jacobson SC, Ramsey JM. (1997) "Microchip device for performing enzyme assays." Anal Chem 69, 3407-3412.
  • Jones DE Jr, Cui DM, Miller DM. (1995)"Expression of beta-galactosidase under the control of the human c-myc promoter in transgenic mice is inhibited by mithramycin." Oncogene 10, 2323-2330.
  • Porstmann T, Kiessig ST. (1992) "Enzyme immunoassay techniques. An overview." J. Immunol. Methods 150, 5-21.
  • Sernetz M, Willems H, Keiner K. (1990) "Dispersive analysis of turnover rates of a CST reactor by flow-through microfluorometry under conditions of growth." Ann. NY Acad. Sci. 613, 333-337.
  • Jefferson RA. (1989) "The GUS reporter gene system." Nature 342, 837-838.
  • Hofmann J, Sernetz M. (1984) "Immobilized enzyme kinetics analyzed by flow-through microfluorimetry. Resorufin-beta-D-galactopyranoside as a new fluorogenic substrate for galactosidase." Anal. Chim. Acta. 163, 67.
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