Resorufin β-D-N-acetylgalactosamine

Product ID: M1037



Unit SizePriceQuantity 
10mg
$98.80
  • Buy 5 for $79.05 each and save 20%

Availability: In stock


Description

Useful fluorescent substrate for galactosaminidase or hexosaminidase activity measurements in Tay-Sachs and Sandhoff diseases. The red fluorescent product, resorufin, exhibits emission at 585 nm when excited at 570nm.

Hexosaminidase (EC 3.2.1.52) is an enzyme involved in the hydrolysis of terminal N-acetyl-D-hexosamine residues in N-acetyl-β-D-hexosaminides including N-acetylglucosaminides and N-acetylgalactosaminides. The enzyme is able to hydrolyze GM2 gangliosides into GM3 gangliosides by removing the N-acetylgalactosamine from gangliosides. Tay-Sachs disease occurs when hexosaminidase A loses its ability to function. People with Tay-Sachs disease are unable to remove the GalNAc residue from the GM2 ganglioside, and as a result, they end up storing 100 to 1000 times more GM2 gangliosides in the brain than the normal person. Resorufin β-D-N-acetylgalactosamine is a specific substrate for the HexA/B isozymes responsible for these activities. For additional uses, see the references below and M0203 for additional details.

Technical Data
SKU M1037
Unit Size 10mg
Detection Method Fluorescence
Molecular Formula C₂₀H₂₀O₈N₂
Molecular Weight 416.38
Soluble In DMSO, H2O
Storage Conditions -20C, Desiccated, Protect From Light

References and Citations

References:

  • "A microplate fluorimetric assay for transfection of the beta-galactosidase reporter gene." Rakmanova VA, MacDonald RC. Anal Biochem 257: 234 (1998).
  • "Preparation of N-acetyl-b-D-glucosamine glycosides of fluorescein derivatives as reagents for determination of N-acetyl-b-D-glucosaminase (NAGase)." Kasai K, Uchida R, Okada K, Yamatsugu N. Jpn. Kokai Tokkyo Koho 8 pp. (1993).
  • "Glycosides having chromophores as substrates for sensitive enzyme analysis. IV. Synthesis of N-acetyl-b-D-glucosaminides of fluorescein derivatives and their application to the rate-assay of N-acetyl-b-D-glucosaminidase." Kasai K, Uchida R, Yamaji N. Chemical & Pharmaceutical Bulletin 41(2): 314-18 (1993).
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