Resorufin Cellobioside

Product ID: M1238

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Fluorogenic substrate for detection of cellulase activity. Cellulase assays are widely used in the biofuels industry.

Resorufin cellobioside is a cellulase substrate that releases the highly fluorescent dye resorufin (EX/EM 571/585 nm) upon enzyme activity. Since resorufin has a low pKa value, enzyme measurements can be obtained at low pH values, similar to physiological pH's found for many cellulases. Cellulase assays are widely used in the biofuels industry.

Technical Data
SKU M1238
Unit Size 5mg
Absorption 571nm
Emission Wavelength 585
Detection Method Fluorescence
Molecular Formula C₂₄H₂₇NO₁₃
Molecular Weight 537.47

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References and Citations


  • Ito Y, Yamanishi M, Ikeuchi A, Imamura C, Matsuyama T. (2015) " Combinatorial screening for transgenic yeasts with high cellulase activities in combination with a tunable expression system." PloS ONE 10(12): e0144870. doi:10.1371/journal.pone.0144870.
  • Tran N, Zielke RA, Vining OB, Azevedo MD, Armstrong DJ, Banowetz GM, McPhail KL, Sikora AE. (2013) "Development of a quantitative assay amenable for high-throughput screening to target the type II secretion system for new treatments against plant-pathogenic bacteria." J Biomol Screen. 18(8):921-9.
  • Rudi K, Strætkvern KO. (2012) "Correlations between Lumbricus terrestris survival and gut microbiota." Microb Ecol Health Dis. 23.
  • Chandrasekaran A, Bharadwaj R, Park JI, Sapra R, Adams PD, Singh AK. (2010) "A microscale platform for integrated cell-free expression and activity screening of cellulases." J Proteome Res. 9(11):5677-83.
  • Paungfoo-Lonhienne C, Rentsch D, Robatzek S, Webb RI, Sagulenko E, Näsholm T, Schmidt S, Lonhienne TG. (2010) "Turning the table: plants consume microbes as a source of nutrients." PLoS ONE. 5(7):e11915. doi:10.1371/journal.pone.0011915.
  • Coleman DJ, Studler MJ, Naleway JJ. (2007) "A long-wavelength fluorescent substrate for continuous fluorometric determination of cellulase activity: resorufin-β-D-cellobioside." Anal. Biochem. 371: 146-153.
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  • Boschker HTS, Cappenberg TE. (1994) "A sensitive method using 4-Methylumbelliferyl-beta-Cellobiose as a Substrate to Measure (1,4)-beta-Glucanase Activity in Sediments." Applied and Environmental Biology, 60(10): 3592-3596.
  • Han SJ, Yoo YJ, Kang HS. (1995) "Characterization of a bifunctional cellulase and Its structural gene." J. Biochem, 270(43): 26012-26019.
  • Chernoglazov VM, Jafarova AN, Klyosov AA. (1989) "Continuous photometric determination of endo-1,4-beta-D-glucanase (cellulase) activity using 4-methylumbelliferyl-beta-D-cellobioside as a substrate." Anal Biochem, 179(1): 186-189.
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