Resorufin ethyl ether

Product ID: M1209

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Microsomal dealkylase and Cytochrome P450 enzyme substrate. Releases the red fluorophore resorufin (Abs. 571 nm; Em. 585 nm) upon enzyme reaction. Routinely used to differentiate the various cytochrome P450 isoforms.

This CYP450 substrate produces the red fluorescent dye resorufin (EX/EM 571/585 nm) upon enzyme activity. This substrate, along with other alkyl resorufins such as the methyl and benzyl ethers (Product M1166) has also been extensively used to differentiate the various isozymes of the Cytochrome P450 system. O-Dealkylations of resorufin and coumarin ethers are mediated by microsomal cytochrome P450 mono-oxygenases from animals, plants and microorganisms.

Technical Data
SKU M1209
CAS Number 5725-91-7
Unit Size 5mg
Alternative Names 7-O-Ethoxyresorufin
Detection Method Fluorescence
Molecular Formula C₁₄H₁₁NO₃
Molecular Weight 241.24
Soluble In DMSO, CH2Cl2
Storage Conditions 2-8C, Desiccated, Protect From Light

References and Citations


  • "Evaluation of alkoxyresorufins as fluorescent substrates for cytochrome P450 BM3 and site-directed mutants." (2005)Lussenburg BM, Babel LC, Vermeulen NP, Commandeur JN., Anal Biochem 341: 148-55.
  • "Purification and biochemical characterization of two major cytochrome P-450 isoforms induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in chick embryo liver." (1994) Rifkind A.B., Kanetoshi A., Orlinick J., Capdevila J.H., Lee C. J., Biol. Chem. 269: 3387-3396.
  • "Methoxyresorufin and benzyloxyresorufin: substrates preferentially metabolized by cytochromes P4501A2 and 2B, respectively, in the rat and mouse." (1993) Nerurkar P.V., Park S.S., Thomas P.E., Nims R.W., Lubet R.A. Biochem. Pharmacol. 46: 933-943.
  • "7-Ethoxyresorufin-O-deethylase activity in human hair roots: a potential marker for toxifying species of cytochrome P-450 isozymes." (1987) Merk H.F., Mukhtar H., Schutte B., Kaufmann I., Das M., Bickers D.R., Biochem. Biophys. Res. Commun. 148: 755-761
  • "Multiple forms of cytochrome P-450 related to forms induced marginally by phenobarbital. Differences in structure and in the metabolism of alkoxyresorufins." (1986)Wolf C.R., Seilman S., Oesch F., Mayer R.T., Burke M.D., Biochem. J. 240: 27-33.
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