Resorufin oleate

Product ID: M1208



Unit SizePriceQuantity 
10mg
$97.54
  • Buy 5 for $78.03 each and save 20%

Availability: In stock


Description

Fluorogenic substrate with red emission for use in detection of intracellular and in vitro lipase activity.

Substrate used in our Long Wavelength Lipase Assay Kit for High-Throughput analysis of lipase activity. Upon activity by the enzyme, the red fluorescent dye resorufin is released which has a low pKa for optimum emission readings at low pH values. The red fluorophore resorufin exhibits an absorption maximum at 571 nm with emission at 585 nm upon enzyme reaction.

Technical Data
SKU M1208
Unit Size 10mg
Detection Method Fluorescence
Molecular Formula C₃₀H₃₉O₄N
Molecular Weight 477.63
Soluble In CHCl3, DMSO

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References and Citations

References:

  • Koster JF, Vaandrager H, van Berkel TJ (1980) "Study of the hydrolysis of 4-methylumbelliferyl oleate by acid lipase and cholesteryl oleate by acid cholesteryl esterase in human leucocytes, fibroblasts and liver." , Biochim Biopys Acta, 618(1):98-105.
  • Kuriyama M, Yoshida H, Suzuki M, Fujiyama J, Igata A, (1990) "Lysosomal acid lipase deficiency in rats: lipid analyses and lipase activities in liver and spleen." J Lipid Res, 31(9):1605-12.
  • Sando GN, Henke VL, (1982) "Recognition and receptor-mediated endocytosis of the lysosomal acid lipase secreted by cultured human fibroblasts." Journal of Lipid Research 23: 114 - 123.
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