Rhodamine 123

Product ID: M0542

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  • Buy 5 for $52.34 each and save 21%

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Cell-permeant, green fluorescent dye that can be used to monitor membrane polarization.

R123 has found extensive use in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA. Rhodamine fluorescence can be used as a measure of membrane polarization in live cell assays both within mitochondria and in bacteria, since it accumulates in membranes in a manner which is dependent on membrane polarization. R123 has also found use as a tracer dye in water to determine the rate and direction of flow and transport since it can be detected easily and inexpensively at very low levels using a fluorometer. The absorption of Rhodamine 123 peaks around 505 nm and luminescence is tunable at around 560 nm when used as a laser dye. Its luminescence quantum yield is very high at about 0.90. R123 has also been used in many applications including bacterial viability and vitality assays, mitochondrial membrane potential assays in apoptotic cells, and for sorting respiratory-deficient yeast cells using flow cytometry.

Technical Data
SKU M0542
CAS Number 62669-70-9
Unit Size 25mg
Absorption 505nm
Emission Wavelength 534
Detection Method Fluorescence
Molecular Formula C₂₁H₁₇N₂O₃Cl
Molecular Weight 380.83
Soluble In DMSO, DMF, MeOH
Storage Conditions Room Temperature

References and Citations


  • Hussein N, Amawi H, Karthikeyan C, Hall FS, Mittal R, Trivedi P, Ashby CR Jr, Tiwari AK. (2017)"The dopamine D(3) receptor antagonists PG01037, NGB2904, SB277011A, and U99194 reverse ABCG2 transporter-mediated drug resistance in cancer cell lines." Cancer Lett. pii: S0304-3835(17)30179-9.
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  • "Rapid Assessment of Bacterial Viability and Vitality by Rhodamine 123 and Flow Cytometry." Kaprelyants AS, Kell DB. J Appl Bacteriol 72: 410 (1992).
  • "Staining of Living Bacteria with Rhodamine 123." Matsuyama T. FEMS Microbiol Lett 21: 153 (1984).
  • "Flow cytometry as a tool to discriminate respiratory-competent and respiratory-deficient yeast cells." Skowronek P, Krummeck G, Haferkamp O, Rodel G. Curr Genet 18: 265-267 (1990).
  • "Temperature-sensitive yeast mutants defective in mitochondrial inheritance." McConnell SJ, Stewart LC, Talin A, Yaffe MP. J Cell Biol 111: 967-976 (1990).
  • "Intracellular ionic variations in the apoptotic death of L cells by inhibitors of cell cycle progression." Barbiero G, Duranti F, Bonelli G, Amenta JS, Baccino FM. Exp Cell Res 217: 410-418 (1995).
  • "Features of apoptotic cells measured by flow cytometry." Darzynkiewicz Z, Bruno S, Del Bino G, Gorczyca W, Hotz MA, Lassota P, Traganos F. Cytometry 13: 795-808 (1992).
  • "Mitochondrial modifications during rat thymocyte apoptosis: a study at the single cell level." Cossarizza A, Kalashnikova G, Grassilli E, Chiappelli F, Salvioli S, Capri M, Barbieri D, Troiano L, Monti D, Franceschi C. Exp Cell Res 214: 323-330 (1994).
  • "Temporal analysis of events associated with programmed cell death (apoptosis) of sympathetic neurons deprived of nerve growth factor." Deckwerth TL, Johnson EM Jr. J Cell Biol 123: 1207-1222 (1993).
  • "Measurement of mitochondrial membrane potential using fluorescent rhodamine derivatives." Scaduto RC Jr, Grotyohann LW. Biophys J 76: 469-477 (1999).
  • "A fast kinetic method for assessing mitochondrial membrane potential in isolated hepatocytes with rhodamine 123 and flow cytometry." Juan G, Cavazzoni M, Saez GT, OConnor JE. Cytometry 5: 335-342 (1994).
  • "Flow Cytometric Analysis of Rhodamine 123 Fluorescence during Modulation of the Membrane Potential in Plant Mitochondria." Petit PX. Plant Physiol 98: 279 (1992).
  • "Localization of mitochondria in living cells with rhodamine 123." Johnson LV, Walsh ML, Chen LB. Proc Natl Acad Sci USA 77: 990-994 (1980).
  • "Scanning microspectrofluorometry of rhodamine 123 in multidrug-resistant cells." Millot JM, Sharonov S, Manfait M. Cytometry 17(1): 50-58 (1994).
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