Rhodol

Product ID: M0370



Unit SizePriceQuantity 
5mg
$98.80
  • Buy 5 for $79.05 each and save 20%

Availability: In stock


Description

Highly fluorescent dye useful for oligonucleotide, antibody and protein labeling studies and for the preparation of peptide and esterase substrates.

Rhodol is a structural hybrid of fluorescein and rhodamine. It retains the pH-sensitive properties conferred by a phenolic hydroxyl group, but the amine substituent reduces the pKa values. Rhodol exhibits a high extinction coefficient (~70,000 cm-1M-1) and high quantum yield (~0.8-0.9) in aqueous solution, with absorption maxima at approximately 490 nm and emission maxima between 520 and 545 nm. As with fluorescein-based dyes, the fluorescence excitation profiles of rhodol-based dyes are pH dependent, which can be useful for ratiometric pH determinations intracellularly. See the references below or call us for more information about your labeling protocol.

Technical Data
SKU M0370
CAS Number 3086-44-0
Unit Size 5mg
Absorption 494nm
Emission Wavelength 521
Extinction 73
Detection Method Fluorescence
Molecular Formula C₂₀H₁₃NO₄
Molecular Weight 331.33
Soluble In MeOH, DMSO, DMF
Storage Conditions Room Temperature, Desiccated

References and Citations

References:

  • "Stains, labels and detection strategies for nucleic acids assays." Kricka, L.J., Ann. Clin. Biochem. (2002)39:114-29

  • "Fluorescent rhodol derivatives: versatile, photostable labels and tracers." Whitaker,J.E., Haugland, R.P., Ryan, D., Hewitt, P.C., Haugland, R.P., Prendergast, F.G., Anal. Biochem. (1992)207:267-279.

  • "Rhodamine dyes and related compounds. XII. Diacetyl derivatives of rhodamine and rhodol; structure of colorless forms of fluoran dyes." Ioffe, I. S., Otten, V. F., Zh. Organ. Khim. (1965)1(2):336-9.

  • "Rhodamine dyes and related compounds. II. Rhodol, its preparation and properties.", Ioffe, I. S., Otten, V. F., Zh. Obshch. Khim.,(1962)32:1196-201.

  • "Fluorescein Substitutes for Microscopy and Imaging." Haugland, R.P., Optical Microscopy for Biology, Herman. B., Jacobson, K., Eds. pp. 143-157 (1990).

>Show more
>Show less

Click here for Newsletter articles about this product


Technical Support

Question about this product? Ask a Scientist!

We pride ourselves on the high quality of our products and want you to get the best possible results from your assays. If you have any questions about this product or need help optimizing your protocol check out the product FAQs below or ask your own question and one of our expert scientists will get back to you asap:


There are currently no frequently asked questions for this product, click the button above to ask a question

Recently Viewed Products
  1. Green FAM Caspases 3 and 7 Assay Kit 25tests

    Green FAM Caspases 3 and 7 Assay Kit 25tests