A lysis buffer used primarily in the extraction and solubilization of cytoplasmic, membrane, and nuclear proteins from cultured mammalian cells.
Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used primarily in the extraction and solubilization of cytoplasmic, membrane, and nuclear proteins from cultured mammalian cells. The low background of this buffer allows its application in a number of assays, such as BCA protein quantification Product M2533, SDS-PAGE, and immunoprecipitation.
Note: This buffer does not contain protease inhibitor. If needed, add just prior to use.
References and Citations
Kirby ED, Kuwahara AA, Messer RL, Wyss-Coray T. (2015) "Adult hippocampal neural stem and progenitor cells regulate the neurogenic niche by secreting VEGF." Proc Natl Acad Sci U S A (112):13 4128-4133.
Pei M, He F, Boyce BM, Kish VL. (2009) "Repair of full-thickness femoral condyle cartilage defects using allogeneic synovial cell-engineered tissue constructs." Osteoarthritis Cartilage (17):6 714-722.
Yellore VS, Rayner SA, Aldave AJ. (2011) "TGFB1-induced extracellular expression of TGFBIp and inhibition of TGFBIp expression by RNA interference in a human corneal epithelial cell line." Invest Ophthalmol Vis Sci (52):2 757-763.
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