RubyGlow™ Red Luciferase

Researchers at Marker Gene Technologies, Inc. have developed a new luciferase gene by utilizing point mutation and codon optimization of the luciferase sequence from the natural luciferase gene isolated from Luciola cruciata (the Japanese firefly). The gene codes for an improved amino acid sequence which exhibits long-wavelength light emission (red color, EM 619 nm), as well as improved thermostability and higher expression levels in mammalian cells compared with other luciferases including the native luciferase derived from the American firefly Photinus pyralis.

The expressed enzyme uses the same substrate, D-Luciferin, that is used with other luciferases, and activity can easily be detected using our convenient MarkerGene™ Live Cell Luciferase Assay Kit (M0626). Additional substrates for use in detecting cloned activity in living tissues and in vivo include the membrane permeant D-Luciferin, ethyl ester (M0906). This makes this new luciferase ideal for use in vivo detection methods of cloned luciferase activity. It can also be used concurrently with vectors such as the pGL3 Photinus pyralis vectors for multiplexed detection of two cloning events in the same cell line.

We have also adapted this new luciferase into a series of kits for use in a microtiter plate format and thus made them amenable to high-throughput applications. These kits produce a red light (EM 619 nm) upon luciferin conversion, while most of the commercially available luciferase kits still use older green light emitting (562 nm) firefly analogs. This unique feature enables the possibility of multiplexing. The new kit components contain no radioisotopes, are not toxic to cells and are environmentally safe. The kits provide enough reagents and a detailed protocol sufficient for 100 reactions in 96-well microplate format

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  1. Fluorescein mono-β-D-galactopyranoside

    Fluorescein mono-β-D-galactopyranoside

    $164.65

    Convenient β-galactosidase fluorescent probe. Especially useful for enzymatic analyses where 1st order kinetics are desired. Learn More
  2. pCdgCAT Mammalian CAT Expression Vector

    pCdgCAT Mammalian CAT Expression Vector

    $126.54

    High copy number eukaryotic vector, pCdgCAT expresses the E. coli Chloramphenicol Acetyltransferase (CAT) gene under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. Very useful for transfection of mammalian cells in culture and for use in other species. Learn More
  3. pDC57 Mammalian Luciferase Expression Vector

    pDC57 Mammalian Luciferase Expression Vector

    $308.90

    This high copy number eukaryotic vector, pDC57, expresses a mutant luciferase gene under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. This vector encodes a mutant enzyme that catalyzes the production of long wavelength (red) light (Em:605) from D-luciferin, M0237. Learn More
  4. pDC99 Mammalian Luciferase Expression Vector

    pDC99 Mammalian Luciferase Expression Vector

    $308.90

    High copy number eukaryotic vector, pDC99 expresses a mutant luciferase gene under the control of the Simian virus 40Early Core (SV40) promoter. This vector encodes a mutant enzyme that catalyzes the production of long wavelength (red) light (Em:605) from D-luciferin, M0237. Learn More
  5. RubyGlow™ Luminescent Bacterial Detection Kit

    RubyGlow™ Luminescent Bacterial Detection Kit

    $135.40

    Unlike other commercially available luciferase kits using green light emitting (562 nm) firefly analogs, this luminescent assay kit produces a red light (EM 619 nm) to easily detect and quantify bacterial cells based upon the generation of ATP in metabolically active cells. Learn More
  6. RubyGlow™ Luminescent Cell Proliferation Assay Kit

    RubyGlow™ Luminescent Cell Proliferation Assay Kit

    $135.40

    Unlike other commercially available luciferase kits using green light emitting (562 nm) firefly analogs, this luminescent assay kit produces a red light (EM 619 nm) for measurement of cell proliferation based upon the generation of ATP in metabolically active cells. Learn More
  7. RubyGlow™ Luminescent Cell Viability Assay Kit

    RubyGlow™ Luminescent Cell Viability Assay Kit

    $135.40

    Unlike other commercially available luciferase kits using green light emitting (562 nm) firefly analogs, this luminescent assay kit produces a red light (EM 619 nm) for measurement of cell viability based upon the generation of ATP in metabolically active cells. Learn More
  8. RubyGlow™ Luminescent Cytotoxicity Assay Kit

    RubyGlow™ Luminescent Cytotoxicity Assay Kit

    $135.40

    Unlike other commercially available luciferase kits using green light emitting (562 nm) firefly analogs, this luminescent assay kit produces a red light (EM 619 nm) for measurement of cytotoxicity based upon the generation of ATP in metabolically active cells. Learn More

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