RubyGlow™ Glo-Gal™ β-Galactosidase Assay Kit

Product ID: M1968



Unit SizePriceQuantity 
1kit
$135.40

Availability: In stock


Description

By coupling the β-Gal assay to the highly sensitive luciferase enzyme lacZ activity can be measured quickly and easy down to extremely low levels of expression. The most sensitive lacZ method available.

Although chromogenic assays of β-galactosidase activity (i.e. X-Gal) have found extensive use as do the application of the fluorogenic substrates (for example FDG) detection of lacZ activity using luminescence can give even greater levels of sensitivity. The sensitive Glo-Gal™ substrate is cleaved first by the β-galactosidase enzyme and then in the presence of ATP, luciferase acts to produce red light output.

Technical Data
SKU M1968
Unit Size 1kit
Detection Method Luminescence

References and Citations

References:

  • Fukuda, S.; Murakami, S.; Tatsumi, H. Recombinant firefly luciferase and their application for the detection of microorganisms. Bioluminescence & Chemiluminescence, Proceedings of the International Symposium, 11th, Pacific Grove, CA, United States, Sept. 6-10, 2000 (2001), Meeting Date 2000, 285-288.
  • Yang, X., Janatova, J., Andrade, J.D. (2005),"Homogeneous enzyme immunoassay modified for application to luminescence-based biosensors." Analytical Biochemistry 336(1), 102-107.
  • Bronstein I, Fortin J, Stanley PE, Stewart GS, Kricka LJ. (1994) "Chemiluminescent and bioluminescent reporter gene assays." Anal. Biochem. 219: 169.
  • Geiger R., Schneider E., Wallenfels K., Miska W., (1992) "A new ultrasensitive bioluminogenic enzyme substrate for beta-galactosidase." Biol. Chem. Hoppe Seyler. 373(12):1187-91.
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