RubyGlow™ Luminescent Bacterial Detection Kit

Product ID: M1573



Unit SizePriceQuantity 
1kit
$135.40

Availability: In stock


Description

Unlike other commercially available luciferase kits using green light emitting (562 nm) firefly analogs, this luminescent assay kit produces a red light (EM 619 nm) to easily detect and quantify bacterial cells based upon the generation of ATP in metabolically active cells.

Each kit components involve no radioisotopes or toxic materials and are environmentally safe. Each kit provides enough reagents and a detailed protocol sufficient for 100 reactions in 96-well microplate format. Bacteria from a number of media or sources can be detected with an extremely high sensitivity (low detection limit). In addition, extended incubation (2-4 hours) of samples at 37ºC can significantly lower the detection limit and increase sensitivity using a protocol that can be completed in one day. Assay is also amenable to high throughput (HTS) applications for analysis of bacterial susceptibility and antibiotic screening.

Technical Data
SKU M1573
Unit Size 1kit
Emission Wavelength 619
Detection Method Luminescence

References and Citations

References:

  • Sherlock O, O'Connell N, Creamer E, Humphreys H. (2009) "Is it really clean? An evaluation of the efficacy of 4 methods for determining hospital cleanliness." J Hosp Infect 72:140–146.
  • Schondelmeyer CW, Dillehay DL, Webb SK, Huerkamp MJ, Mook DM, Pullium JK. (2006) "Investigation of appropriate sanitization frequency for rodent caging accessories: evidence supporting less-frequent cleaning." J Am Assoc Lab Anim Sci 45:40–43.
  • Sharpe AN, Woodrow MN, Jackson AK (1970) “Adenosine triphosphate (ATP) levels in foods contaminated by bacteria”. J. Appl. Bacteriol. 33: 758-767
  • Stannard CJ, Wood JM (1983) “The rapid estimation of microbial contamination of raw meat by measurement of adenosine triphosphate (ATP)”. J. Appl. Bacteriol. 55:429-438
  • Stanley PE (1986) “Extraction of adenosine triphosphate from microbial and somatic cells”. Methods Enzymol. 133:14-22
  • Karl DM (1980) “Cellular nucleotide measurement and applications in microbial ecology”. Microbiol. Rev. 44:739-96
  • McElroy WD, DeLuca MA (1983) “Firefly and bacterial luminescence: Basic science and applications”. J. Applied Biochem. 5:197-209
  • Ulrich PG, Wannlund JC (1984) “Bioluminescence in the microbiology laboratory”. American Clinical Products Rev.
  • Tuncan EU, Martin SE (1987) “Lysostaphin lysis procedure for detection of Staphylococcus aureus by the firefly bioluminescent ATP method”. Applied and Environmental Microbiology 53: 88-91
  • Lundin A, Thore A, (1975) “Analytical information obtainable by evaluation of the time course of firefly bioluminescence in the assay of ATP”. Anal. Biochem. 66:47-63.
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