SophiaGreen™ 421

Product ID: M1931



Unit SizePriceQuantity 
50 μl
$173.50
  • Buy 5 for $138.80 each and save 21%

Availability: In stock


Description

Excellent counterstain for DNA and chromatin, providing high increase in fluorescence upon binding to nucleic acid.

A unit contains 50 μl of a 1 mM solution in DMSO. Can be excited using excitation filters appropriate for green-fluorescent dyes. This green fluorescent stain is membrane impermeant, and thus does not stain live cells. Suggested starting concentration for dead cell determination is 50nM.

Click here for excitation and emission spectrum.


Technical Data
SKU M1931
Unit Size 50 μl
Absorption 491nm
Emission Wavelength 509
Detection Method Fluorescence
Soluble In DMSO
Storage Conditions -20C, Protect From Light
Notes

Replaces Product M1716.


References and Citations

References:

  • Fürstenberg A, Julliard MD, Deligeorgiev TG, Gadjev NI, Vasilev AA, Vauthey E. (2006) “Ultrafast excited-state dynamics of DNA fluorescent intercalators: new insight into the fluorescence enhancement mechanism.” J Am Chem Soc. 128(23):7661-9.
  • Rye HS, Glazer AN. (1995) “Interaction of dimeric intercalating dyes with single-stranded DNA.” Nucleic Acids Res. 23(7):1215-22.
  • Rye HS, Dabora JM, Quesada MA, Mathies RA, Glazer AN.(1993) “Fluorometric assay using dimeric dyes for double- and single-stranded DNA and RNA with picogram sensitivity.” Anal Biochem. 208(1):144-50.
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