Tetramethylrhodamine, methyl ester (TMRM)

Product ID: M1693



Unit SizePriceQuantity 
10 mg
$81.86
  • Buy 5 for $65.49 each and save 20%

Availability: In stock


Description

Tetramethylrhodamine, methyl ester (TMRM) is a cell-permeant, cationic, red-orange fluorescent dye that is used to measure mitochondrial membrane potential.

TMRM (Product M1693) accumulates within healthy, negatively charged mitochondrial organelles, exhibiting a red-orange fluorescence (EM 573 nm). When the mitochondrial membrane potential collapses in apoptotic or metabolically stressed cells, the dye is dispersed throughout the cell cytosol exhibiting minimal fluorescence. Such mitochondrial depolarization is a hallmark of cell health and viability and its measurement is indicative of a variety of neurodegenerative diseases such as Parkinson‘s and Alzheimer‘s Diseases as well as age-related mitochondrial malfunction. TMRM-stained cells can be analyzed using flow cytometry, fluorescence microscopy, and fluorescence plate reader evaluation methodologies.

Technical Data
SKU M1693
CAS Number 115532-49-5
Unit Size 10 mg
Absorption 548
Emission Wavelength 573
Extinction 110
Detection Method Fluorescence
Molecular Formula C₂₅H₂₅ClN₂O₃
Molecular Weight 436.93
Soluble In DMSO, DMF, MeOH, EtOH
Storage Conditions 2-8C, Desiccated, Protect From Light
Notes Sold as the more water-soluble chloride salt.

References and Citations

References:

  • Honarnejad K, Daschner A, Gehring A. P, Szybinska A, Giese A, Kuznicki J, Bracher F, Herms J, (2014) "Identification of tetrahydrocarbazoles as novel multifactorial drug candidates for treatment of Alzheimer's disease." Translational Psychiatry 4(12): e489.
  • Plasek J, Denksteinova B, Sureau F (1993) "Assessment of membrane potential using confocal microspectrofluorometry." Journal of Fluorescence 3(3): 157-159.
  • Loew LM (1996) "Potentiometric dyes: New modalities for optical imaging of membrane potential>" Proceedings of SPIE-The International Society for Optical Engineering 2678(Optical Diagnostics of Living Cells and Biofluids), 80-87.
  • ZhangJ, Khvorostov I, Hong JS, Oktay Y, Vergnes L, Nuebel E, Wahjudi PN, Setoguchi K, Wang G, Do A, Jung HJ, J McCaffery M, Kurland I, Reue K, Lee WNP, Koehler CM, Teitell MA(2011) "UCP2 regulates energy metabolism and differentiation potential of human pluripotent stem cells." EMBO J., 30(24): 4851-5022.
  • Scaduto RC, Grotyohann LW (1999) "Measurement of mitochondrial membrane potential using fluorescent rhodamine derivatives." Biophys J. 76(1 Pt 1):469-477.
  • Chazotte B, (2011) "Labeling Mitochondria with ™RM or ™RE." Cold Spring Harb Protocols, Cold Spring Harbor Laboratory Press, doi:10.1101/pdb.prot5641.
  • O'Reilly CM, Fogarty KE, Drummond RM, Tuft RA, Walsh JV (2003) "Quantitative Analysis of Spontaneous Mitochondrial Depolarizations." Biophys. J. 85(5): 3350–3357.
  • Collins TJ, Berridge MJ, Lipp P, Bootman MD (2002) "Mitochondria are morphologically and functionally heterogeneous within cells." EMBO J. 21(7): 1616-1627.
  • Duchen MR, Leyssens A, Crompton M (1998) "Transient mitochondrial depolarizations reflect focal sarcoplasmic reticular calcium release in single rat cardiomyocytes." J. Cell Biol. 142(4): 975-988.
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